Lactococcus phage AM4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Audreyjarvisvirus; Lactococcus virus AM4

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JKN0|A0A1W6JKN0_9CAUD Uncharacterized protein OS=Lactococcus phage AM4 OX=1965472 GN=AM4_123 PE=4 SV=1
MM1 pKa = 7.4YY2 pKa = 8.8NTIWLDD8 pKa = 3.45SEE10 pKa = 3.84QDD12 pKa = 3.15DD13 pKa = 4.51WIVEE17 pKa = 4.2EE18 pKa = 4.13LAGMLGVHH26 pKa = 6.33CHH28 pKa = 7.16DD29 pKa = 4.31IDD31 pKa = 4.46ASIDD35 pKa = 3.05KK36 pKa = 10.78HH37 pKa = 6.59EE38 pKa = 4.04YY39 pKa = 10.14LKK41 pKa = 10.93AISEE45 pKa = 4.84LYY47 pKa = 10.7CEE49 pKa = 4.05MLEE52 pKa = 4.35YY53 pKa = 10.68EE54 pKa = 4.66LDD56 pKa = 3.59EE57 pKa = 4.81EE58 pKa = 5.17GTFKK62 pKa = 10.84VDD64 pKa = 2.78SLYY67 pKa = 11.11SPRR70 pKa = 11.84EE71 pKa = 3.89YY72 pKa = 11.27NFDD75 pKa = 3.39TDD77 pKa = 4.71HH78 pKa = 7.96IVITWDD84 pKa = 3.0SDD86 pKa = 3.72TFTVEE91 pKa = 3.99EE92 pKa = 4.13MQEE95 pKa = 3.95EE96 pKa = 4.39LKK98 pKa = 11.04EE99 pKa = 4.41LISTNDD105 pKa = 2.99NKK107 pKa = 10.26MDD109 pKa = 3.64MDD111 pKa = 4.74MDD113 pKa = 3.83TEE115 pKa = 4.07MWIDD119 pKa = 3.43RR120 pKa = 11.84GYY122 pKa = 11.18EE123 pKa = 3.88LYY125 pKa = 11.47SNMTAYY131 pKa = 9.68KK132 pKa = 10.69YY133 pKa = 10.25KK134 pKa = 10.68GQEE137 pKa = 3.39LWFDD141 pKa = 3.46MDD143 pKa = 4.65ADD145 pKa = 5.26DD146 pKa = 3.95IAKK149 pKa = 10.53VKK151 pKa = 10.53GG152 pKa = 3.41

Molecular weight: 18.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JKR3|A0A1W6JKR3_9CAUD Structural protein OS=Lactococcus phage AM4 OX=1965472 GN=AM4_182 PE=4 SV=1
MM1 pKa = 7.08FKK3 pKa = 10.67KK4 pKa = 10.45RR5 pKa = 11.84ITRR8 pKa = 11.84LIILIVSYY16 pKa = 10.71LFLVGVSWIFPTIHH30 pKa = 5.22MTNNIFKK37 pKa = 10.42NIAILLVGQPILNMFIAGCIAMVSIFLALLAYY69 pKa = 10.03AIIYY73 pKa = 10.12AVVKK77 pKa = 9.83WVMKK81 pKa = 10.32GDD83 pKa = 3.59KK84 pKa = 10.4

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

193

0

193

38868

29

3280

201.4

23.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.958 ± 0.304

0.715 ± 0.093

6.836 ± 0.181

7.929 ± 0.242

4.469 ± 0.2

5.717 ± 0.234

1.464 ± 0.108

7.67 ± 0.189

9.293 ± 0.181

8.323 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.108

6.442 ± 0.177

2.215 ± 0.113

3.296 ± 0.243

3.46 ± 0.13

6.602 ± 0.261

6.136 ± 0.205

6.242 ± 0.164

1.081 ± 0.083

4.608 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski