Synechococcus phage S-RIM8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Neptunevirus; Synechococcus virus SRIM8

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7SAI4|A0A1D7SAI4_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIM8 OX=756278 GN=RW010115_044 PE=4 SV=1
MM1 pKa = 7.54ARR3 pKa = 11.84QGLLAQSKK11 pKa = 9.38PLAATDD17 pKa = 3.58TLLYY21 pKa = 9.81SAPVDD26 pKa = 3.79TSASAVLKK34 pKa = 10.2IANDD38 pKa = 4.23GTGSAYY44 pKa = 10.53SVALRR49 pKa = 11.84DD50 pKa = 3.63YY51 pKa = 11.23DD52 pKa = 3.43QDD54 pKa = 3.93LVLDD58 pKa = 3.83SSSYY62 pKa = 10.93LLHH65 pKa = 7.13KK66 pKa = 10.61GDD68 pKa = 3.53IVTSYY73 pKa = 10.54RR74 pKa = 11.84VSIDD78 pKa = 3.17TAVSVGSFTPGAQFVSSSGEE98 pKa = 3.77STMKK102 pKa = 10.59FEE104 pKa = 4.59SFYY107 pKa = 11.21VPPLTTIYY115 pKa = 11.13VKK117 pKa = 8.26TTSIRR122 pKa = 11.84VISLEE127 pKa = 4.21LVDD130 pKa = 3.83TATGEE135 pKa = 4.26FNVGDD140 pKa = 4.03TVSKK144 pKa = 10.86GVAPDD149 pKa = 3.27NTDD152 pKa = 2.93AVIYY156 pKa = 9.93DD157 pKa = 3.63VNGDD161 pKa = 4.81FITIGPSTINGLGTEE176 pKa = 4.66FVDD179 pKa = 4.28GDD181 pKa = 4.89AITSSSTASGTIAAGGISATEE202 pKa = 3.65NKK204 pKa = 10.21FIFSEE209 pKa = 4.53TTVGGTYY216 pKa = 10.39SRR218 pKa = 11.84TVSDD222 pKa = 4.18LFSDD226 pKa = 3.9RR227 pKa = 11.84VYY229 pKa = 11.0RR230 pKa = 11.84FDD232 pKa = 4.6VSDD235 pKa = 3.28TSMTGLLFALSEE247 pKa = 4.45TEE249 pKa = 3.94NGEE252 pKa = 3.97WGPDD256 pKa = 3.07GLAPTDD262 pKa = 4.39PNDD265 pKa = 3.95ALDD268 pKa = 4.1GGIEE272 pKa = 4.0YY273 pKa = 9.25TIGKK277 pKa = 5.34TTNGTAGSGGAYY289 pKa = 8.99VQYY292 pKa = 11.58DD293 pKa = 3.56MAANTSLPTSLFWYY307 pKa = 7.33EE308 pKa = 3.77TDD310 pKa = 3.37LTTAANASYY319 pKa = 11.07GGGQDD324 pKa = 3.28SFSTSSEE331 pKa = 3.75VTYY334 pKa = 10.79TEE336 pKa = 5.39FYY338 pKa = 10.69AYY340 pKa = 10.37DD341 pKa = 4.68LDD343 pKa = 4.38GTWTNNVDD351 pKa = 3.45SFEE354 pKa = 5.75DD355 pKa = 3.44NFITYY360 pKa = 7.85TVEE363 pKa = 4.1GQTSGPYY370 pKa = 10.25GVVSDD375 pKa = 4.03YY376 pKa = 11.31TGTSLKK382 pKa = 10.3IILGVGSAEE391 pKa = 3.94FAGSNTFFDD400 pKa = 4.02VPKK403 pKa = 11.04SNTADD408 pKa = 3.87RR409 pKa = 11.84NTVTVSSVTTAKK421 pKa = 9.57STIAADD427 pKa = 3.59SYY429 pKa = 11.69LVDD432 pKa = 4.44GNTNSANNTDD442 pKa = 3.79TVTSLVVGPGQRR454 pKa = 11.84VHH456 pKa = 6.54VNSVTANNVFSLIGFEE472 pKa = 4.94DD473 pKa = 4.66GSSEE477 pKa = 4.1YY478 pKa = 7.39TTRR481 pKa = 11.84VYY483 pKa = 11.13GQTT486 pKa = 3.3

Molecular weight: 50.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7SCL2|A0A1D7SCL2_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIM8 OX=756278 GN=RW010115_102 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84ALTKK6 pKa = 9.73TRR8 pKa = 11.84SAKK11 pKa = 10.25FYY13 pKa = 9.61SNQVKK18 pKa = 9.8FLLLIVIAVLFWNSNDD34 pKa = 2.83ARR36 pKa = 11.84RR37 pKa = 11.84FTSDD41 pKa = 3.17RR42 pKa = 11.84LNDD45 pKa = 3.33AAEE48 pKa = 4.35FVRR51 pKa = 11.84PANNEE56 pKa = 3.21IRR58 pKa = 11.84ISFF61 pKa = 3.8

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

222

0

222

54758

37

6395

246.7

27.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.042 ± 0.2

0.882 ± 0.093

6.616 ± 0.148

6.331 ± 0.28

4.304 ± 0.122

7.639 ± 0.304

1.443 ± 0.132

6.538 ± 0.214

5.711 ± 0.391

7.277 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.219

5.785 ± 0.205

3.861 ± 0.13

3.875 ± 0.084

4.124 ± 0.168

6.967 ± 0.224

7.349 ± 0.46

6.846 ± 0.201

1.123 ± 0.098

4.299 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski