Parabacteroides sp. AF48-14

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; unclassified Parabacteroides

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3786 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A415ZP48|A0A415ZP48_9BACT Uncharacterized protein OS=Parabacteroides sp. AF48-14 OX=2292052 GN=DW083_19970 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 5.61AVTLSEE8 pKa = 3.54ARR10 pKa = 11.84VYY12 pKa = 10.88VGTYY16 pKa = 9.56NKK18 pKa = 10.08YY19 pKa = 10.6NNGSLFGKK27 pKa = 9.26WLDD30 pKa = 3.72LSDD33 pKa = 5.06YY34 pKa = 10.69SDD36 pKa = 3.45KK37 pKa = 11.77DD38 pKa = 3.52EE39 pKa = 4.48FLEE42 pKa = 4.84ACRR45 pKa = 11.84EE46 pKa = 4.08LHH48 pKa = 7.34KK49 pKa = 10.91DD50 pKa = 3.54DD51 pKa = 4.58QDD53 pKa = 3.96PEE55 pKa = 5.08FMFQDD60 pKa = 3.9YY61 pKa = 11.28EE62 pKa = 4.42NIPEE66 pKa = 4.13ALISEE71 pKa = 4.48SWLSEE76 pKa = 3.76KK77 pKa = 10.49FFEE80 pKa = 5.55LRR82 pKa = 11.84DD83 pKa = 4.29AIEE86 pKa = 4.48KK87 pKa = 10.62LSEE90 pKa = 4.15TEE92 pKa = 3.79QEE94 pKa = 5.56AFFVWCDD101 pKa = 3.32HH102 pKa = 6.55HH103 pKa = 8.25NSDD106 pKa = 5.34ISEE109 pKa = 4.05EE110 pKa = 4.1DD111 pKa = 3.42ADD113 pKa = 4.39DD114 pKa = 5.21LISSFEE120 pKa = 4.1DD121 pKa = 4.17EE122 pKa = 4.47YY123 pKa = 11.31QGEE126 pKa = 4.44YY127 pKa = 10.22KK128 pKa = 10.73DD129 pKa = 4.14EE130 pKa = 3.79EE131 pKa = 5.05DD132 pKa = 3.37YY133 pKa = 11.08AYY135 pKa = 10.08EE136 pKa = 4.47IVEE139 pKa = 3.94QCYY142 pKa = 10.53DD143 pKa = 3.45LPEE146 pKa = 4.03FAKK149 pKa = 10.18TYY151 pKa = 10.45FDD153 pKa = 3.79YY154 pKa = 11.39SAFARR159 pKa = 11.84DD160 pKa = 5.17LFITDD165 pKa = 2.97YY166 pKa = 11.18WMDD169 pKa = 3.15NGFVFRR175 pKa = 11.84CAA177 pKa = 3.58

Molecular weight: 21.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A416A6L9|A0A416A6L9_9BACT Beta-galactosidase OS=Parabacteroides sp. AF48-14 OX=2292052 GN=DW083_10180 PE=3 SV=1
MM1 pKa = 7.6FEE3 pKa = 5.7LGPLWGFEE11 pKa = 3.81KK12 pKa = 10.59HH13 pKa = 5.71VLYY16 pKa = 11.1NKK18 pKa = 9.68LRR20 pKa = 11.84QIFLQLTNNPIPLFVVYY37 pKa = 10.4SFYY40 pKa = 10.75INKK43 pKa = 8.46KK44 pKa = 7.99TMTTNRR50 pKa = 11.84TKK52 pKa = 10.55RR53 pKa = 11.84RR54 pKa = 11.84PSEE57 pKa = 4.03VYY59 pKa = 10.3LFRR62 pKa = 11.84TIDD65 pKa = 3.19TLIFRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84SAGRR76 pKa = 11.84DD77 pKa = 3.19ATADD81 pKa = 4.0LYY83 pKa = 10.97RR84 pKa = 11.84AAGNWFRR91 pKa = 11.84KK92 pKa = 8.7FRR94 pKa = 11.84NGRR97 pKa = 11.84DD98 pKa = 3.28LCLLGMTPSLVDD110 pKa = 4.14ALSLSCRR117 pKa = 11.84PGKK120 pKa = 10.32SS121 pKa = 2.84

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3786

0

3786

1319893

22

2158

348.6

39.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.22 ± 0.048

1.15 ± 0.015

5.677 ± 0.028

6.544 ± 0.041

4.589 ± 0.026

6.901 ± 0.037

1.731 ± 0.017

6.791 ± 0.035

6.617 ± 0.032

9.173 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.019

5.063 ± 0.032

3.805 ± 0.024

3.402 ± 0.024

4.623 ± 0.037

6.139 ± 0.026

5.703 ± 0.042

6.53 ± 0.036

1.256 ± 0.016

4.457 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski