Bat coronavirus HKU9-3

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Nobecovirus; Rousettus bat coronavirus HKU9

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3EXI5|A3EXI5_BCHK9 Membrane protein OS=Bat coronavirus HKU9-3 OX=424369 GN=M PE=3 SV=1
MM1 pKa = 7.32YY2 pKa = 9.76EE3 pKa = 4.44LVSADD8 pKa = 3.47TSVVIANVLVVIILCLVVVIVGCALLLILQFILGTCGCLFSIICKK53 pKa = 8.67PTILVYY59 pKa = 11.02NKK61 pKa = 9.42FRR63 pKa = 11.84NEE65 pKa = 3.76SLLNEE70 pKa = 4.09QEE72 pKa = 4.04EE73 pKa = 4.36LLII76 pKa = 5.21

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3EXI7|A3EXI7_BCHK9 Uncharacterized protein NS7a OS=Bat coronavirus HKU9-3 OX=424369 GN=NS7a PE=4 SV=1
MM1 pKa = 7.62SGRR4 pKa = 11.84NKK6 pKa = 9.69PRR8 pKa = 11.84PTSQPKK14 pKa = 8.48VTFKK18 pKa = 10.97QEE20 pKa = 3.89SDD22 pKa = 3.52GSDD25 pKa = 3.37SEE27 pKa = 4.52SEE29 pKa = 3.89RR30 pKa = 11.84RR31 pKa = 11.84NGNRR35 pKa = 11.84SGARR39 pKa = 11.84SKK41 pKa = 11.36NNNGRR46 pKa = 11.84GGAPKK51 pKa = 10.08PEE53 pKa = 4.27KK54 pKa = 10.13PKK56 pKa = 10.56AAPPQNVSWFAPLVQTGKK74 pKa = 10.3QDD76 pKa = 3.27LRR78 pKa = 11.84FPRR81 pKa = 11.84GQGVPISQGVDD92 pKa = 3.34PIYY95 pKa = 11.18NHH97 pKa = 6.99GYY99 pKa = 7.74WVRR102 pKa = 11.84TQRR105 pKa = 11.84TFQKK109 pKa = 10.39GGKK112 pKa = 8.03PVSANPRR119 pKa = 11.84WYY121 pKa = 10.1FYY123 pKa = 9.78YY124 pKa = 10.26TGTGRR129 pKa = 11.84YY130 pKa = 9.52GDD132 pKa = 3.52MRR134 pKa = 11.84YY135 pKa = 8.01GTKK138 pKa = 10.57NPDD141 pKa = 3.26IYY143 pKa = 10.66WVGEE147 pKa = 3.54EE148 pKa = 4.13GANVNRR154 pKa = 11.84VGDD157 pKa = 3.64MGTRR161 pKa = 11.84NPNNDD166 pKa = 3.06AAISVQLADD175 pKa = 5.5GIPKK179 pKa = 10.23GFYY182 pKa = 10.47AEE184 pKa = 4.29GRR186 pKa = 11.84NSRR189 pKa = 11.84GNSRR193 pKa = 11.84NSSRR197 pKa = 11.84NSSRR201 pKa = 11.84ASSRR205 pKa = 11.84GGSRR209 pKa = 11.84PGSRR213 pKa = 11.84GASPGRR219 pKa = 11.84ATPSGSGAEE228 pKa = 3.75PWMAYY233 pKa = 9.69LVSKK237 pKa = 11.29LEE239 pKa = 3.98TLEE242 pKa = 4.54AKK244 pKa = 10.82VNGTKK249 pKa = 10.06SEE251 pKa = 4.19TKK253 pKa = 10.7APVQVTKK260 pKa = 10.85SAAAEE265 pKa = 3.82NAKK268 pKa = 10.28KK269 pKa = 10.1LRR271 pKa = 11.84HH272 pKa = 5.99KK273 pKa = 8.81RR274 pKa = 11.84TPHH277 pKa = 6.14KK278 pKa = 10.85GSGVTMNYY286 pKa = 9.19GRR288 pKa = 11.84RR289 pKa = 11.84GPGDD293 pKa = 3.4LEE295 pKa = 5.04GNFGDD300 pKa = 3.87QTMLKK305 pKa = 10.3LGVDD309 pKa = 4.07DD310 pKa = 4.51PRR312 pKa = 11.84FPAVAQMAPNVASFIFMSHH331 pKa = 6.6LSTRR335 pKa = 11.84EE336 pKa = 3.75EE337 pKa = 4.41NDD339 pKa = 3.98ALWLQYY345 pKa = 10.58KK346 pKa = 10.07GAIKK350 pKa = 10.38LPKK353 pKa = 9.74DD354 pKa = 3.61DD355 pKa = 5.0PNYY358 pKa = 9.59EE359 pKa = 3.76QWTKK363 pKa = 11.25LLAEE367 pKa = 4.58NLNAYY372 pKa = 9.72KK373 pKa = 10.37DD374 pKa = 4.17FPPPEE379 pKa = 4.14PKK381 pKa = 9.44KK382 pKa = 10.51DD383 pKa = 3.35KK384 pKa = 10.53KK385 pKa = 10.65KK386 pKa = 10.72KK387 pKa = 10.23EE388 pKa = 4.24EE389 pKa = 3.97ISSDD393 pKa = 3.52TVVFEE398 pKa = 4.65DD399 pKa = 4.62ASTGTDD405 pKa = 3.19QAVVKK410 pKa = 10.26VWVKK414 pKa = 11.36DD415 pKa = 3.64EE416 pKa = 4.17GAQTDD421 pKa = 4.1DD422 pKa = 3.32EE423 pKa = 4.62WLGGDD428 pKa = 3.25DD429 pKa = 3.35TVYY432 pKa = 10.84EE433 pKa = 4.38EE434 pKa = 4.62EE435 pKa = 4.59DD436 pKa = 4.11DD437 pKa = 3.96RR438 pKa = 11.84PKK440 pKa = 9.38TQRR443 pKa = 11.84RR444 pKa = 11.84HH445 pKa = 5.15KK446 pKa = 10.06KK447 pKa = 9.76RR448 pKa = 11.84NSTASRR454 pKa = 11.84VTIADD459 pKa = 3.43PMNATSEE466 pKa = 4.26RR467 pKa = 11.84SS468 pKa = 3.23

Molecular weight: 51.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

9532

76

6923

1191.5

132.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.753 ± 0.209

3.063 ± 0.413

5.277 ± 0.47

3.714 ± 0.564

4.144 ± 0.274

6.295 ± 0.544

1.836 ± 0.168

4.091 ± 0.589

4.522 ± 0.725

9.851 ± 0.754

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.205

4.994 ± 0.792

4.658 ± 0.51

3.294 ± 0.333

4.438 ± 0.696

6.756 ± 0.576

6.504 ± 0.328

9.987 ± 0.709

1.311 ± 0.168

5.046 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski