Salmovirus WFRC1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6EUV6|A0A1W6EUV6_9VIRU Protease OS=Salmovirus WFRC1 OX=1981916 PE=4 SV=1
MM1 pKa = 7.07FAWSRR6 pKa = 11.84PNSGLASAEE15 pKa = 3.56ILKK18 pKa = 9.88FRR20 pKa = 11.84KK21 pKa = 9.45PGFILSCEE29 pKa = 3.75QTARR33 pKa = 11.84LEE35 pKa = 4.12KK36 pKa = 10.64AKK38 pKa = 10.75EE39 pKa = 3.84KK40 pKa = 10.62VAEE43 pKa = 4.2ILCGATGCCRR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 10.23RR56 pKa = 11.84LMDD59 pKa = 3.67LQVDD63 pKa = 4.41LSRR66 pKa = 11.84SPGYY70 pKa = 9.11PYY72 pKa = 10.66KK73 pKa = 10.58YY74 pKa = 10.11VYY76 pKa = 10.06RR77 pKa = 11.84SKK79 pKa = 10.94KK80 pKa = 10.26DD81 pKa = 3.37SLEE84 pKa = 4.09DD85 pKa = 4.16PIIYY89 pKa = 10.16DD90 pKa = 3.21RR91 pKa = 11.84VMTGNCWRR99 pKa = 11.84DD100 pKa = 3.32PAPLWDD106 pKa = 3.69AFGKK110 pKa = 10.58FEE112 pKa = 4.42ILPVEE117 pKa = 4.34KK118 pKa = 10.44ALKK121 pKa = 9.14RR122 pKa = 11.84CRR124 pKa = 11.84LITSPPVDD132 pKa = 3.2VALCVSMWYY141 pKa = 9.92EE142 pKa = 4.01DD143 pKa = 3.48QNEE146 pKa = 4.18RR147 pKa = 11.84LMSLCEE153 pKa = 3.84SSPYY157 pKa = 10.66GVGISKK163 pKa = 8.22FTGSWNRR170 pKa = 11.84LMSRR174 pKa = 11.84MPNVLWQCDD183 pKa = 3.56VSQFDD188 pKa = 3.34ACIRR192 pKa = 11.84PLLMSAVYY200 pKa = 9.93DD201 pKa = 3.6VRR203 pKa = 11.84EE204 pKa = 4.39LLFEE208 pKa = 5.3DD209 pKa = 4.16IPRR212 pKa = 11.84EE213 pKa = 3.96YY214 pKa = 11.4YY215 pKa = 10.16EE216 pKa = 6.62FRR218 pKa = 11.84DD219 pKa = 3.27QWLDD223 pKa = 3.31FLIDD227 pKa = 3.76GSFVDD232 pKa = 3.84HH233 pKa = 6.64HH234 pKa = 6.71TGDD237 pKa = 3.91LYY239 pKa = 11.38SVHH242 pKa = 6.85GGNKK246 pKa = 9.52SGSPNTSSDD255 pKa = 3.1NTIANMIVLCYY266 pKa = 11.14SMMSVGVNPMEE277 pKa = 4.44TPFVCYY283 pKa = 10.42GDD285 pKa = 5.51DD286 pKa = 4.15LLLCGSVPEE295 pKa = 4.92DD296 pKa = 2.78VWAAYY301 pKa = 9.22TSMGMIIKK309 pKa = 10.09PGACKK314 pKa = 9.72RR315 pKa = 11.84VMKK318 pKa = 10.41TEE320 pKa = 3.83VDD322 pKa = 4.15FLSCKK327 pKa = 8.34SLKK330 pKa = 10.36RR331 pKa = 11.84YY332 pKa = 7.81GQYY335 pKa = 10.22VPVWNSYY342 pKa = 8.47KK343 pKa = 10.31AAYY346 pKa = 10.77SMMVTDD352 pKa = 5.55SKK354 pKa = 11.83GEE356 pKa = 3.9LLRR359 pKa = 11.84GSRR362 pKa = 11.84FKK364 pKa = 11.19SLLLEE369 pKa = 4.16SLFVAEE375 pKa = 5.4FGVLSEE381 pKa = 4.24FARR384 pKa = 11.84LNGFFYY390 pKa = 10.86SRR392 pKa = 11.84SLLEE396 pKa = 4.62CIFFGMEE403 pKa = 3.83VEE405 pKa = 5.13DD406 pKa = 4.05KK407 pKa = 11.42KK408 pKa = 11.46EE409 pKa = 3.88MPGKK413 pKa = 10.15KK414 pKa = 9.74RR415 pKa = 11.84NTKK418 pKa = 8.57TKK420 pKa = 10.52QSLKK424 pKa = 10.35VPASRR429 pKa = 11.84PQKK432 pKa = 9.09QKK434 pKa = 9.23RR435 pKa = 11.84PRR437 pKa = 11.84RR438 pKa = 11.84RR439 pKa = 11.84RR440 pKa = 11.84QAGSRR445 pKa = 11.84TGQTDD450 pKa = 3.18DD451 pKa = 4.08SQWFMIHH458 pKa = 6.67RR459 pKa = 11.84EE460 pKa = 4.25AISTSTQSGVLFSSILHH477 pKa = 6.45PSLFPATPYY486 pKa = 9.02YY487 pKa = 9.72TRR489 pKa = 11.84CSNFTHH495 pKa = 7.13RR496 pKa = 11.84IEE498 pKa = 4.47RR499 pKa = 11.84SWEE502 pKa = 3.48FRR504 pKa = 11.84IMITTASFTGSRR516 pKa = 11.84VAVIPVNDD524 pKa = 4.5PSPDD528 pKa = 3.14AVMSEE533 pKa = 4.23TVAFQQVANGRR544 pKa = 11.84AAIATSTGASDD555 pKa = 3.19QRR557 pKa = 11.84SFVKK561 pKa = 10.05MYY563 pKa = 10.82GATTTLSNANPSVQSSLVGFSVGTFFVYY591 pKa = 10.91LLDD594 pKa = 4.17PPIGLAQGAALLVTVLARR612 pKa = 11.84VALTVVGPFTGFLTFEE628 pKa = 4.93ANPTDD633 pKa = 3.69HH634 pKa = 6.98GAPPVEE640 pKa = 4.48LKK642 pKa = 10.69ISDD645 pKa = 3.72VTKK648 pKa = 10.07DD649 pKa = 3.0RR650 pKa = 11.84SFITHH655 pKa = 6.39SKK657 pKa = 10.46SSYY660 pKa = 10.51LDD662 pKa = 3.21FGRR665 pKa = 11.84YY666 pKa = 8.2LAIEE670 pKa = 4.83GPSVTLDD677 pKa = 3.23SAEE680 pKa = 4.03WAVKK684 pKa = 10.34PEE686 pKa = 3.98FSRR689 pKa = 11.84IYY691 pKa = 10.96ASLSPPADD699 pKa = 2.57SWYY702 pKa = 10.81NNQDD706 pKa = 3.3EE707 pKa = 4.92KK708 pKa = 11.29IMPEE712 pKa = 4.77FYY714 pKa = 10.58TEE716 pKa = 3.93GDD718 pKa = 3.19LGGGVHH724 pKa = 7.0VMIGWPTIQDD734 pKa = 2.85ARR736 pKa = 11.84TFTVSPSSVVNGRR749 pKa = 11.84ALGFGSTDD757 pKa = 3.02SKK759 pKa = 11.64AKK761 pKa = 10.45KK762 pKa = 9.31KK763 pKa = 9.64WSEE766 pKa = 3.81TWSGGSEE773 pKa = 3.77SGQRR777 pKa = 11.84SIFFVAIGAQTVDD790 pKa = 4.42AMCCDD795 pKa = 3.33SFLGQRR801 pKa = 11.84LGALSLNSDD810 pKa = 3.76PSLLEE815 pKa = 3.81PP816 pKa = 5.14

Molecular weight: 90.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6EUV6|A0A1W6EUV6_9VIRU Protease OS=Salmovirus WFRC1 OX=1981916 PE=4 SV=1
MM1 pKa = 7.35SPKK4 pKa = 9.53RR5 pKa = 11.84DD6 pKa = 3.56QPVSVMTIAPYY17 pKa = 10.73VLLGVVGGFAYY28 pKa = 10.41AAMSSACSDD37 pKa = 4.54CEE39 pKa = 4.19HH40 pKa = 6.75TCQGTFTDD48 pKa = 5.74GMCLMCQKK56 pKa = 11.13GEE58 pKa = 4.11LQCEE62 pKa = 4.39SCEE65 pKa = 4.04QSAAEE70 pKa = 4.42TPTKK74 pKa = 8.51TRR76 pKa = 11.84KK77 pKa = 8.18RR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.02YY81 pKa = 10.01KK82 pKa = 10.37KK83 pKa = 9.99EE84 pKa = 4.23CIASLWGEE92 pKa = 4.06VRR94 pKa = 11.84EE95 pKa = 5.25GFALWSPLAAVFGLHH110 pKa = 7.08HH111 pKa = 6.56YY112 pKa = 9.71RR113 pKa = 11.84DD114 pKa = 3.59AMLASKK120 pKa = 10.44ALEE123 pKa = 4.15GLEE126 pKa = 4.09GLVKK130 pKa = 10.4KK131 pKa = 10.29VWHH134 pKa = 6.75LGDD137 pKa = 3.37KK138 pKa = 9.62PKK140 pKa = 10.86PEE142 pKa = 4.52FSISDD147 pKa = 4.09CINRR151 pKa = 11.84VNKK154 pKa = 10.36RR155 pKa = 11.84ILSMGDD161 pKa = 2.96GYY163 pKa = 11.27EE164 pKa = 4.17ADD166 pKa = 4.44DD167 pKa = 4.98EE168 pKa = 4.71IMGYY172 pKa = 7.49STEE175 pKa = 4.23GSASRR180 pKa = 11.84ARR182 pKa = 11.84EE183 pKa = 3.56RR184 pKa = 11.84YY185 pKa = 7.92EE186 pKa = 3.77NSKK189 pKa = 10.27NRR191 pKa = 11.84YY192 pKa = 8.37DD193 pKa = 3.94RR194 pKa = 11.84EE195 pKa = 4.32GEE197 pKa = 3.91RR198 pKa = 11.84KK199 pKa = 9.32FIEE202 pKa = 4.1RR203 pKa = 11.84AKK205 pKa = 10.77LGFFGGCIDD214 pKa = 4.32KK215 pKa = 11.02VSNVCTNGLGFGLAAALFGCFIYY238 pKa = 10.11RR239 pKa = 11.84QRR241 pKa = 11.84VRR243 pKa = 11.84RR244 pKa = 11.84SLIYY248 pKa = 9.51GTDD251 pKa = 3.6AVKK254 pKa = 9.14KK255 pKa = 9.04TCDD258 pKa = 2.49KK259 pKa = 10.6WMKK262 pKa = 10.44KK263 pKa = 8.39DD264 pKa = 3.34TEE266 pKa = 4.22SRR268 pKa = 11.84ISGSHH273 pKa = 5.78TFTGCDD279 pKa = 3.41KK280 pKa = 10.43ICRR283 pKa = 11.84AVAQISTEE291 pKa = 4.07SGSVGTAFKK300 pKa = 10.57CGKK303 pKa = 8.51YY304 pKa = 9.44WITAGHH310 pKa = 5.08VVKK313 pKa = 10.93SGTAYY318 pKa = 11.01LHH320 pKa = 5.81TPSGKK325 pKa = 9.84EE326 pKa = 3.36ATEE329 pKa = 4.13VIKK332 pKa = 9.68FFPTANDD339 pKa = 5.28GITVMASKK347 pKa = 10.2YY348 pKa = 10.56SPMGLRR354 pKa = 11.84SVPVSVLTSALPGFVVGCQNGDD376 pKa = 3.26PHH378 pKa = 8.12LSTGLVEE385 pKa = 4.62PDD387 pKa = 2.97GTHH390 pKa = 5.52YY391 pKa = 11.04CSTTFGHH398 pKa = 6.2SGAPVVAHH406 pKa = 6.23NGVVVGVHH414 pKa = 5.64ILGDD418 pKa = 3.71EE419 pKa = 4.24DD420 pKa = 4.41VNGYY424 pKa = 10.75LPMTRR429 pKa = 11.84DD430 pKa = 3.07IISFLRR436 pKa = 11.84EE437 pKa = 4.0GPSAPVKK444 pKa = 10.34KK445 pKa = 10.22KK446 pKa = 10.63AVVPQDD452 pKa = 3.86VVSMAPITDD461 pKa = 4.31LLSQGMQQASVSNTLEE477 pKa = 4.01KK478 pKa = 10.46PGPCSHH484 pKa = 6.19GQGLIVDD491 pKa = 5.0LPLQRR496 pKa = 11.84SSSLEE501 pKa = 3.93SLDD504 pKa = 3.37SSSRR508 pKa = 11.84VSKK511 pKa = 10.53QHH513 pKa = 5.82DD514 pKa = 3.47LKK516 pKa = 10.91RR517 pKa = 11.84RR518 pKa = 11.84KK519 pKa = 9.95KK520 pKa = 9.28KK521 pKa = 7.19WQKK524 pKa = 10.75SSAVQPGVAAASVV537 pKa = 3.15

Molecular weight: 58.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1353

537

816

676.5

74.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.095 ± 0.335

2.809 ± 0.454

5.174 ± 0.206

5.1 ± 0.071

4.582 ± 0.765

8.056 ± 1.012

1.7 ± 0.564

4.065 ± 0.096

5.913 ± 0.839

7.761 ± 0.541

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.956 ± 0.217

2.513 ± 0.289

5.174 ± 0.438

3.03 ± 0.084

5.691 ± 0.296

10.421 ± 0.343

5.617 ± 0.019

7.539 ± 0.407

1.626 ± 0.316

3.178 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski