bacterium HR26

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5YQ05|A0A2H5YQ05_9BACT Decaprenyl-phosphate phosphoribosyltransferase OS=bacterium HR26 OX=2035421 GN=HRbin26_00417 PE=4 SV=1
MM1 pKa = 7.64DD2 pKa = 4.11QYY4 pKa = 10.65TGPGGSRR11 pKa = 11.84DD12 pKa = 3.78AEE14 pKa = 4.39PEE16 pKa = 4.05DD17 pKa = 4.69EE18 pKa = 4.48YY19 pKa = 11.67QPVPPFASRR28 pKa = 11.84PPGSSPFGSEE38 pKa = 4.1RR39 pKa = 11.84QFTYY43 pKa = 9.33QTDD46 pKa = 3.04EE47 pKa = 4.96RR48 pKa = 11.84YY49 pKa = 5.96WTDD52 pKa = 3.47YY53 pKa = 10.82IRR55 pKa = 11.84IAAPVLGVILMLGLAWFWISNLLGDD80 pKa = 4.21RR81 pKa = 11.84AEE83 pKa = 4.3SQPTPEE89 pKa = 5.3AGSLPNVITGPTATPEE105 pKa = 4.24TTPSGPIIITTVSPASTPQSPPPSGTIGPGATVVVANTDD144 pKa = 3.23GAGVNLRR151 pKa = 11.84AAPSTSAEE159 pKa = 4.02IVQTLPEE166 pKa = 4.41GTEE169 pKa = 3.77LTIVGEE175 pKa = 4.48SVSAEE180 pKa = 4.14GYY182 pKa = 8.33VWWPVEE188 pKa = 4.17SGDD191 pKa = 3.72LSGYY195 pKa = 9.53VVADD199 pKa = 3.45YY200 pKa = 9.72LQPATPTPP208 pKa = 3.74

Molecular weight: 21.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5YTS8|A0A2H5YTS8_9BACT Uncharacterized protein OS=bacterium HR26 OX=2035421 GN=HRbin26_01739 PE=4 SV=1
MM1 pKa = 7.48AKK3 pKa = 9.95KK4 pKa = 10.46GKK6 pKa = 9.75ADD8 pKa = 4.52RR9 pKa = 11.84IIITLEE15 pKa = 3.69CTEE18 pKa = 4.05CRR20 pKa = 11.84EE21 pKa = 4.75RR22 pKa = 11.84NYY24 pKa = 8.02TTQKK28 pKa = 10.25NRR30 pKa = 11.84RR31 pKa = 11.84NDD33 pKa = 3.6PGRR36 pKa = 11.84LEE38 pKa = 4.08LKK40 pKa = 10.0KK41 pKa = 10.54YY42 pKa = 10.21CPRR45 pKa = 11.84CRR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 5.68RR50 pKa = 11.84LHH52 pKa = 7.44RR53 pKa = 11.84EE54 pKa = 3.49TRR56 pKa = 3.37

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2404

0

2404

729898

30

1474

303.6

33.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.913 ± 0.061

0.784 ± 0.016

4.885 ± 0.032

6.848 ± 0.054

3.073 ± 0.03

8.447 ± 0.042

2.062 ± 0.023

4.79 ± 0.041

1.617 ± 0.027

11.639 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.019

1.786 ± 0.022

6.164 ± 0.038

3.557 ± 0.034

8.814 ± 0.057

4.581 ± 0.031

4.899 ± 0.032

8.34 ± 0.041

1.539 ± 0.024

2.445 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski