Gordonia rhizosphera NBRC 16068

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia rhizosphera

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6X1L0|K6X1L0_9ACTN Putative oxidoreductase OS=Gordonia rhizosphera NBRC 16068 OX=1108045 GN=GORHZ_186_00600 PE=4 SV=1
MM1 pKa = 7.19LTRR4 pKa = 11.84RR5 pKa = 11.84SVGYY9 pKa = 10.5DD10 pKa = 3.35SEE12 pKa = 4.85SDD14 pKa = 3.46NAPTRR19 pKa = 11.84LAAYY23 pKa = 10.42LSDD26 pKa = 3.75EE27 pKa = 4.16PVAAGVDD34 pKa = 3.33IGVSDD39 pKa = 3.66QTFEE43 pKa = 4.02AWLQAASVYY52 pKa = 11.18DD53 pKa = 3.61NVVPQLASAVCADD66 pKa = 3.75DD67 pKa = 5.02HH68 pKa = 7.18LAGLLANDD76 pKa = 4.06TQQ78 pKa = 3.74

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6WNA0|K6WNA0_9ACTN Putative esterase OS=Gordonia rhizosphera NBRC 16068 OX=1108045 GN=GORHZ_233_00320 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNGRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.92GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5930

0

5930

1941780

19

4653

327.5

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.661 ± 0.041

0.798 ± 0.01

6.729 ± 0.027

5.317 ± 0.028

3.019 ± 0.019

8.877 ± 0.026

2.285 ± 0.017

4.594 ± 0.017

2.014 ± 0.022

9.503 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.014

2.095 ± 0.014

5.584 ± 0.022

2.69 ± 0.017

7.404 ± 0.032

5.563 ± 0.018

6.387 ± 0.025

8.828 ± 0.033

1.47 ± 0.013

2.077 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski