Serratia phage phiMAM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Miltonvirus; Serratia virus MAM1

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7YH08|K7YH08_9CAUD dUTP diphosphatase OS=Serratia phage phiMAM1 OX=1262513 GN=MAM_151 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84YY3 pKa = 9.54LPQNQVIEE11 pKa = 4.33NLVDD15 pKa = 3.62VYY17 pKa = 10.86IVNALISSNPHH28 pKa = 5.77LAPYY32 pKa = 10.01FYY34 pKa = 11.38YY35 pKa = 10.79NLNLTVHH42 pKa = 5.66SVEE45 pKa = 4.35VVDD48 pKa = 6.66DD49 pKa = 4.16EE50 pKa = 5.43LLVANIEE57 pKa = 4.47FYY59 pKa = 11.18LVDD62 pKa = 4.27DD63 pKa = 4.98NGTPVCKK70 pKa = 9.75PVFCKK75 pKa = 10.56VDD77 pKa = 3.34VEE79 pKa = 4.77NYY81 pKa = 9.87FPLADD86 pKa = 4.97FFNMDD91 pKa = 4.76DD92 pKa = 4.14GSMLDD97 pKa = 3.22RR98 pKa = 11.84DD99 pKa = 3.85EE100 pKa = 6.17FEE102 pKa = 5.2DD103 pKa = 3.25SDD105 pKa = 3.87YY106 pKa = 11.62RR107 pKa = 11.84GFF109 pKa = 3.9

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7YB51|K7YB51_9CAUD Uncharacterized protein OS=Serratia phage phiMAM1 OX=1262513 GN=MAM_099 PE=4 SV=1
MM1 pKa = 7.25NAVEE5 pKa = 4.12QNVLFSKK12 pKa = 10.51HH13 pKa = 5.22QGHH16 pKa = 7.04IKK18 pKa = 10.66AKK20 pKa = 8.99DD21 pKa = 3.49LKK23 pKa = 10.73VVYY26 pKa = 9.96LKK28 pKa = 10.13TGSILQRR35 pKa = 11.84RR36 pKa = 11.84DD37 pKa = 3.22VVIRR41 pKa = 11.84WSRR44 pKa = 11.84GYY46 pKa = 8.13WHH48 pKa = 7.05VYY50 pKa = 9.98DD51 pKa = 4.62VVDD54 pKa = 4.02KK55 pKa = 11.12VVSPSSRR62 pKa = 11.84SRR64 pKa = 11.84HH65 pKa = 5.52RR66 pKa = 11.84LLLDD70 pKa = 3.48ALKK73 pKa = 10.64SVHH76 pKa = 6.4

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

198

0

198

47997

37

1615

242.4

27.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.455 ± 0.203

1.135 ± 0.078

6.396 ± 0.119

6.438 ± 0.215

4.238 ± 0.1

6.973 ± 0.214

1.865 ± 0.092

6.005 ± 0.111

6.169 ± 0.176

7.638 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.973 ± 0.089

5.132 ± 0.138

3.875 ± 0.107

3.792 ± 0.116

5.09 ± 0.121

6.102 ± 0.161

5.932 ± 0.191

7.561 ± 0.164

1.396 ± 0.071

3.836 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski