Pseudidiomarina salinarum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Pseudidiomarina

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094ISP6|A0A094ISP6_9GAMM Uncharacterized protein OS=Pseudidiomarina salinarum OX=435908 GN=IDSA_11460 PE=4 SV=1
MM1 pKa = 7.9EE2 pKa = 5.15YY3 pKa = 8.87NTSEE7 pKa = 4.54LCDD10 pKa = 4.82LYY12 pKa = 10.59PDD14 pKa = 3.51MVDD17 pKa = 3.02VVEE20 pKa = 5.04PMFIHH25 pKa = 6.51YY26 pKa = 9.64GGQVSFGGQLVTVKK40 pKa = 10.53CFEE43 pKa = 4.64DD44 pKa = 3.11KK45 pKa = 11.35GLIEE49 pKa = 5.29EE50 pKa = 4.59VLQQPGVGKK59 pKa = 10.62VLLVDD64 pKa = 4.24GGGSTRR70 pKa = 11.84RR71 pKa = 11.84ALIDD75 pKa = 3.18IGIAEE80 pKa = 4.18QALDD84 pKa = 4.03NGWEE88 pKa = 4.51GIVCYY93 pKa = 10.66GSVRR97 pKa = 11.84DD98 pKa = 3.97VDD100 pKa = 4.89ALDD103 pKa = 3.7EE104 pKa = 4.31LEE106 pKa = 4.45IGIMALASIPVGASSEE122 pKa = 4.6GVGEE126 pKa = 3.85TDD128 pKa = 3.26VAVNFGGVTFLPEE141 pKa = 3.46DD142 pKa = 4.47HH143 pKa = 7.26IYY145 pKa = 11.47ADD147 pKa = 3.57ATGVILSPEE156 pKa = 4.15PLDD159 pKa = 4.12IEE161 pKa = 4.39

Molecular weight: 17.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094IUR5|A0A094IUR5_9GAMM Tol-Pal system protein TolQ OS=Pseudidiomarina salinarum OX=435908 GN=tolQ PE=3 SV=1
MM1 pKa = 7.89LITGWLWCLAGGMVAGLLAGMLGIGGGLVIVPLLIYY37 pKa = 9.55LLPILGVEE45 pKa = 4.26QEE47 pKa = 5.27LVMQMAIATSLATIVMTTLSSARR70 pKa = 11.84SHH72 pKa = 6.69LKK74 pKa = 10.08HH75 pKa = 6.42GQISWFWVRR84 pKa = 11.84RR85 pKa = 11.84LIPGLMVGAVLGAWLATLLKK105 pKa = 10.33PEE107 pKa = 3.74WLQRR111 pKa = 11.84IFAGVLMILAVRR123 pKa = 11.84MLIPQRR129 pKa = 11.84TGEE132 pKa = 4.18PLTDD136 pKa = 3.23VSKK139 pKa = 11.32RR140 pKa = 11.84LMALISACMGTLAALVGIGGGSLTVPLLQRR170 pKa = 11.84LRR172 pKa = 11.84VPIRR176 pKa = 11.84QAIAVSSVGSLSIGLTAALAFIVLGQRR203 pKa = 11.84LEE205 pKa = 4.33GSAGLLGYY213 pKa = 8.84VHH215 pKa = 6.89FEE217 pKa = 3.26AWLAISIASVVCAPFGATLTQKK239 pKa = 10.33IPVKK243 pKa = 10.28RR244 pKa = 11.84LQLGFALFLVFISVNLVIGG263 pKa = 3.75

Molecular weight: 27.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2100

0

2100

702921

51

1870

334.7

37.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.933 ± 0.056

0.826 ± 0.015

5.867 ± 0.041

6.325 ± 0.046

3.819 ± 0.035

7.255 ± 0.048

2.21 ± 0.027

5.715 ± 0.04

3.951 ± 0.052

10.74 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.456 ± 0.024

3.398 ± 0.034

4.367 ± 0.03

4.933 ± 0.053

5.895 ± 0.048

5.731 ± 0.039

5.19 ± 0.032

7.092 ± 0.044

1.369 ± 0.023

2.927 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski