Pseudonocardia thermophila

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5850 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6TQH6|A0A1M6TQH6_PSETH TIGR00288 family protein OS=Pseudonocardia thermophila OX=1848 GN=SAMN05443637_108184 PE=4 SV=1
MM1 pKa = 7.62AVQLDD6 pKa = 4.19DD7 pKa = 3.78QLMDD11 pKa = 4.22ILACPADD18 pKa = 3.97HH19 pKa = 7.15APLRR23 pKa = 11.84VGTAADD29 pKa = 3.6PAADD33 pKa = 3.57VLTCTACGLRR43 pKa = 11.84YY44 pKa = 9.03PVVDD48 pKa = 5.37GIPVLLADD56 pKa = 3.91EE57 pKa = 4.9ALPAEE62 pKa = 4.53AAADD66 pKa = 3.88GAGAA70 pKa = 4.01

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6VI91|A0A1M6VI91_PSETH NUDIX domain-containing protein OS=Pseudonocardia thermophila OX=1848 GN=SAMN05443637_112143 PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.65HH19 pKa = 4.47GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIIANRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 11.14GRR42 pKa = 11.84VRR44 pKa = 11.84LSAA47 pKa = 3.96

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5850

0

5850

1837469

27

7715

314.1

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.388 ± 0.059

0.798 ± 0.01

5.965 ± 0.028

5.761 ± 0.029

2.647 ± 0.016

9.2 ± 0.032

2.188 ± 0.015

3.54 ± 0.024

1.614 ± 0.024

10.318 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.013

1.538 ± 0.015

6.412 ± 0.028

2.571 ± 0.018

8.342 ± 0.03

4.435 ± 0.022

5.868 ± 0.022

9.481 ± 0.031

1.425 ± 0.012

1.824 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski