Mycobacterium phage Jolie2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EGQ4|W8EGQ4_9CAUD Uncharacterized protein OS=Mycobacterium phage Jolie2 OX=1458831 GN=Jolie2_1 PE=4 SV=1
MM1 pKa = 7.12STTDD5 pKa = 3.16AEE7 pKa = 4.28RR8 pKa = 11.84NAWATLDD15 pKa = 3.85AAIEE19 pKa = 4.15ACFAIPDD26 pKa = 3.96PEE28 pKa = 4.48RR29 pKa = 11.84PAGAVPVDD37 pKa = 3.08ACLIVGVQHH46 pKa = 6.28VEE48 pKa = 3.59NDD50 pKa = 3.65GARR53 pKa = 11.84IGYY56 pKa = 9.36VEE58 pKa = 4.67LFPRR62 pKa = 11.84AGGQPSYY69 pKa = 9.05VTRR72 pKa = 11.84GLLVDD77 pKa = 4.55ADD79 pKa = 4.03HH80 pKa = 7.16LLTRR84 pKa = 11.84IEE86 pKa = 4.5DD87 pKa = 3.8QAEE90 pKa = 4.21HH91 pKa = 7.68DD92 pKa = 4.85DD93 pKa = 3.96DD94 pKa = 4.41TPP96 pKa = 4.39

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8ED18|W8ED18_9CAUD RecE OS=Mycobacterium phage Jolie2 OX=1458831 GN=Jolie2_42 PE=4 SV=1
MM1 pKa = 7.52MYY3 pKa = 11.11GNGKK7 pKa = 9.82RR8 pKa = 11.84KK9 pKa = 9.09GARR12 pKa = 11.84LANPRR17 pKa = 11.84DD18 pKa = 3.56PMVRR22 pKa = 11.84EE23 pKa = 3.78ALTRR27 pKa = 11.84KK28 pKa = 10.23CPDD31 pKa = 3.29CAAEE35 pKa = 4.03PEE37 pKa = 4.62FWCVGIAEE45 pKa = 4.21NSRR48 pKa = 11.84TKK50 pKa = 10.59GRR52 pKa = 11.84RR53 pKa = 11.84LTRR56 pKa = 11.84LHH58 pKa = 6.69FARR61 pKa = 11.84ARR63 pKa = 11.84FIDD66 pKa = 3.31SDD68 pKa = 4.27APVKK72 pKa = 10.75AGARR76 pKa = 3.65

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

14380

47

1340

231.9

24.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.185 ± 0.589

0.89 ± 0.191

7.204 ± 0.366

5.647 ± 0.483

2.942 ± 0.17

9.332 ± 0.728

2.058 ± 0.201

4.402 ± 0.219

3.574 ± 0.241

7.587 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.093 ± 0.103

2.872 ± 0.158

6.349 ± 0.331

3.122 ± 0.127

6.808 ± 0.508

4.701 ± 0.282

6.259 ± 0.317

6.933 ± 0.215

1.961 ± 0.135

2.079 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski