Microviridae Bog5275_51

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UMC9|A0A0G2UMC9_9VIRU DNA pilot protein VP2 OS=Microviridae Bog5275_51 OX=1655648 PE=4 SV=1
MM1 pKa = 7.42SKK3 pKa = 10.21QRR5 pKa = 11.84IINTGQLNLTEE16 pKa = 5.06RR17 pKa = 11.84PTPNYY22 pKa = 8.79PHH24 pKa = 6.89NSFNLSHH31 pKa = 6.02SVKK34 pKa = 10.69LSFNYY39 pKa = 10.18GNLVPVMVMDD49 pKa = 3.81VVPGDD54 pKa = 3.63RR55 pKa = 11.84VHH57 pKa = 7.38IDD59 pKa = 3.23HH60 pKa = 7.16EE61 pKa = 4.57CLLRR65 pKa = 11.84YY66 pKa = 9.65QPMIAPPMQRR76 pKa = 11.84VKK78 pKa = 10.75VRR80 pKa = 11.84SEE82 pKa = 4.18SFFVPYY88 pKa = 10.3RR89 pKa = 11.84LLWPNFEE96 pKa = 4.4SAMGQRR102 pKa = 11.84DD103 pKa = 3.69VSDD106 pKa = 4.15PAPAFPTISVDD117 pKa = 3.49PEE119 pKa = 3.93VGISKK124 pKa = 10.57SSLANYY130 pKa = 10.12LGMPVLPVQPGGSTYY145 pKa = 10.76VYY147 pKa = 8.61PTMNAFRR154 pKa = 11.84FAAYY158 pKa = 10.11QKK160 pKa = 10.55IFADD164 pKa = 4.37WYY166 pKa = 10.67ADD168 pKa = 3.43EE169 pKa = 5.72DD170 pKa = 4.02LQIDD174 pKa = 4.22SVFVPLSDD182 pKa = 3.87GDD184 pKa = 3.73NGSIEE189 pKa = 4.02TSGYY193 pKa = 10.57GFLNQRR199 pKa = 11.84LYY201 pKa = 11.32DD202 pKa = 3.85LDD204 pKa = 4.14YY205 pKa = 9.97FTVAKK210 pKa = 9.58PWPQKK215 pKa = 10.05GSEE218 pKa = 4.24VVIPALNTQVPVSVIDD234 pKa = 3.38STAQQRR240 pKa = 11.84LYY242 pKa = 9.52QTVNGTYY249 pKa = 10.66ADD251 pKa = 3.55AGAPNIEE258 pKa = 4.65HH259 pKa = 7.77DD260 pKa = 4.33SMPIQAAFQDD270 pKa = 3.77GGGFLINLDD279 pKa = 3.89PKK281 pKa = 10.96GSLGINPADD290 pKa = 3.43WAGAAGTIEE299 pKa = 3.97QLRR302 pKa = 11.84VAEE305 pKa = 4.06AMQRR309 pKa = 11.84FLEE312 pKa = 4.47ADD314 pKa = 3.13SRR316 pKa = 11.84GGTRR320 pKa = 11.84YY321 pKa = 10.57GEE323 pKa = 4.43LIYY326 pKa = 11.08AHH328 pKa = 6.78FGVMNPDD335 pKa = 3.22ARR337 pKa = 11.84LQYY340 pKa = 10.43SEE342 pKa = 4.63YY343 pKa = 10.74LGSTSQQISFGEE355 pKa = 4.14VLNTTGTSTAPQGAMAGHH373 pKa = 6.76GVSAHH378 pKa = 5.9KK379 pKa = 9.69TNAPYY384 pKa = 10.12HH385 pKa = 6.3CNVTEE390 pKa = 4.24HH391 pKa = 6.46GVILTLMSVLPDD403 pKa = 2.98TGYY406 pKa = 10.12YY407 pKa = 9.68QGVPKK412 pKa = 10.53EE413 pKa = 3.91FFKK416 pKa = 11.1NDD418 pKa = 3.18RR419 pKa = 11.84LDD421 pKa = 3.98YY422 pKa = 10.6LWPEE426 pKa = 3.8YY427 pKa = 11.43AMLGEE432 pKa = 3.94QPINKK437 pKa = 9.7GEE439 pKa = 4.3VYY441 pKa = 10.19WDD443 pKa = 3.44VDD445 pKa = 3.55PSEE448 pKa = 5.92GSDD451 pKa = 3.53QNTQEE456 pKa = 3.7FGYY459 pKa = 10.35VGRR462 pKa = 11.84FNEE465 pKa = 4.56YY466 pKa = 7.51RR467 pKa = 11.84TQYY470 pKa = 11.05NRR472 pKa = 11.84VCGDD476 pKa = 3.33MAVSLDD482 pKa = 3.52FWQQDD487 pKa = 3.08RR488 pKa = 11.84KK489 pKa = 10.23FAGLPPLDD497 pKa = 3.86GADD500 pKa = 4.16FLTCVPDD507 pKa = 3.98DD508 pKa = 3.7ALNRR512 pKa = 11.84IFAVIDD518 pKa = 3.38ADD520 pKa = 4.04ADD522 pKa = 3.57HH523 pKa = 7.09LLAHH527 pKa = 7.06WWHH530 pKa = 6.09GVTVEE535 pKa = 3.99RR536 pKa = 11.84CLPRR540 pKa = 11.84LAMPSLL546 pKa = 3.86

Molecular weight: 60.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UK08|A0A0G2UK08_9VIRU Major capsid protein VP1 OS=Microviridae Bog5275_51 OX=1655648 PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.16AIFAAPVPLIRR14 pKa = 11.84NHH16 pKa = 6.14FNYY19 pKa = 10.42DD20 pKa = 3.23YY21 pKa = 11.31AEE23 pKa = 4.13TFGRR27 pKa = 11.84VSPNKK32 pKa = 10.54SMTVPGAVKK41 pKa = 10.3SLAWMIDD48 pKa = 2.83RR49 pKa = 11.84FTRR52 pKa = 11.84NLAMPPAPAAFNTEE66 pKa = 4.18LDD68 pKa = 3.69LSPFDD73 pKa = 3.77RR74 pKa = 11.84LNVFEE79 pKa = 5.94KK80 pKa = 10.27IDD82 pKa = 3.48LSRR85 pKa = 11.84AYY87 pKa = 9.57KK88 pKa = 10.19RR89 pKa = 11.84RR90 pKa = 11.84ALSLRR95 pKa = 11.84KK96 pKa = 9.45QLDD99 pKa = 3.54DD100 pKa = 5.0KK101 pKa = 11.35RR102 pKa = 11.84QADD105 pKa = 3.52ANAEE109 pKa = 3.81QEE111 pKa = 4.14RR112 pKa = 11.84FIEE115 pKa = 3.95EE116 pKa = 3.9RR117 pKa = 11.84AALRR121 pKa = 11.84AIEE124 pKa = 4.02LSKK127 pKa = 10.6LAKK130 pKa = 9.64DD131 pKa = 4.09AKK133 pKa = 10.65DD134 pKa = 3.62GQAA137 pKa = 3.86

Molecular weight: 15.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1480

137

546

296.0

32.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.757 ± 2.588

0.811 ± 0.366

6.149 ± 0.491

3.784 ± 0.541

4.122 ± 0.381

6.959 ± 0.754

2.095 ± 0.47

3.986 ± 0.635

3.986 ± 0.809

7.973 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.149

5.608 ± 1.102

6.081 ± 0.597

5.405 ± 0.654

5.473 ± 1.039

6.081 ± 0.383

5.405 ± 0.804

6.486 ± 0.725

1.351 ± 0.36

3.581 ± 0.853
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski