Euphorbia leaf curl Guangxi virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1KQS9|A1KQS9_9GEMI AC4 protein OS=Euphorbia leaf curl Guangxi virus OX=412688 GN=AC4 PE=3 SV=1
MM1 pKa = 7.33PQPKK5 pKa = 9.9KK6 pKa = 10.8FLINAKK12 pKa = 10.13NYY14 pKa = 8.98FLTYY18 pKa = 7.85PHH20 pKa = 7.15CSLTKK25 pKa = 10.61EE26 pKa = 4.06EE27 pKa = 5.34ALSQLQNLEE36 pKa = 3.94TPTNKK41 pKa = 9.89KK42 pKa = 9.99FIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.34EE52 pKa = 5.86DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 5.24GKK67 pKa = 10.07FKK69 pKa = 10.55CQNNRR74 pKa = 11.84FFDD77 pKa = 3.66LTSPSRR83 pKa = 11.84SAHH86 pKa = 5.0FHH88 pKa = 6.45PNIQGAKK95 pKa = 9.86SSTDD99 pKa = 2.85VKK101 pKa = 11.28AYY103 pKa = 8.67MEE105 pKa = 4.5KK106 pKa = 10.82DD107 pKa = 3.27GDD109 pKa = 4.09VLDD112 pKa = 4.81HH113 pKa = 7.15GVFQIDD119 pKa = 3.19GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.09AAALNILKK151 pKa = 10.25EE152 pKa = 4.07KK153 pKa = 10.42APKK156 pKa = 10.58DD157 pKa = 3.54FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.05RR171 pKa = 11.84IFAPPLEE178 pKa = 4.42VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.96EE195 pKa = 4.22LEE197 pKa = 4.03DD198 pKa = 3.5WVSEE202 pKa = 4.19NVRR205 pKa = 11.84DD206 pKa = 3.6AAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.55SIVIEE220 pKa = 4.33GDD222 pKa = 3.1SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.05KK312 pKa = 10.49EE313 pKa = 3.9YY314 pKa = 11.13LNEE317 pKa = 4.19EE318 pKa = 4.25KK319 pKa = 10.94NSALKK324 pKa = 10.56NWASKK329 pKa = 10.47NATFITLEE337 pKa = 3.98EE338 pKa = 4.04PLYY341 pKa = 10.77SGSHH345 pKa = 5.41QSPTPNSQEE354 pKa = 3.68EE355 pKa = 4.27ANQEE359 pKa = 4.05EE360 pKa = 4.92EE361 pKa = 4.45GG362 pKa = 3.76

Molecular weight: 40.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1KQS6|A1KQS6_9GEMI Replication enhancer OS=Euphorbia leaf curl Guangxi virus OX=412688 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.39RR4 pKa = 11.84PADD7 pKa = 3.29IVTSTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 8.83TSRR30 pKa = 11.84AAAPTVLVTNKK41 pKa = 9.79RR42 pKa = 11.84KK43 pKa = 9.79SWVNRR48 pKa = 11.84PMYY51 pKa = 9.89RR52 pKa = 11.84KK53 pKa = 8.98PRR55 pKa = 11.84MYY57 pKa = 11.01RR58 pKa = 11.84MFRR61 pKa = 11.84SPDD64 pKa = 3.23VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 10.35VQSFDD79 pKa = 3.26AKK81 pKa = 11.06NDD83 pKa = 3.27IGHH86 pKa = 6.11MGKK89 pKa = 10.22VICLSDD95 pKa = 3.3VTRR98 pKa = 11.84GVGLTHH104 pKa = 7.14RR105 pKa = 11.84VGKK108 pKa = 9.78RR109 pKa = 11.84FCVKK113 pKa = 10.05SLYY116 pKa = 10.31FVGKK120 pKa = 9.14IWMDD124 pKa = 3.34EE125 pKa = 4.02NIKK128 pKa = 10.41VKK130 pKa = 10.64NHH132 pKa = 5.76TNTVMFWIVRR142 pKa = 11.84DD143 pKa = 3.87RR144 pKa = 11.84RR145 pKa = 11.84PSGTPNDD152 pKa = 3.78FQQVFNVYY160 pKa = 10.67DD161 pKa = 4.09NEE163 pKa = 4.37PSTATVKK170 pKa = 10.57NDD172 pKa = 2.71HH173 pKa = 7.04RR174 pKa = 11.84DD175 pKa = 3.12RR176 pKa = 11.84FQVLRR181 pKa = 11.84RR182 pKa = 11.84FQATVTGGQYY192 pKa = 10.18AAKK195 pKa = 8.87EE196 pKa = 3.58QAIIRR201 pKa = 11.84KK202 pKa = 8.72FYY204 pKa = 10.37RR205 pKa = 11.84VNNHH209 pKa = 4.56VVYY212 pKa = 10.62NHH214 pKa = 5.8QEE216 pKa = 3.47AGKK219 pKa = 10.41YY220 pKa = 8.39EE221 pKa = 4.09NHH223 pKa = 6.52TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.99ATLKK246 pKa = 8.67VRR248 pKa = 11.84SYY250 pKa = 10.98FYY252 pKa = 11.25DD253 pKa = 3.13SVTNN257 pKa = 3.98

Molecular weight: 29.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1101

97

362

183.5

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.268 ± 0.767

2.452 ± 0.315

4.995 ± 0.466

4.905 ± 0.929

4.632 ± 0.472

4.45 ± 0.377

4.178 ± 0.504

5.268 ± 0.862

6.54 ± 0.528

6.812 ± 0.904

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.499

5.995 ± 0.756

6.267 ± 0.852

5.086 ± 0.628

6.358 ± 1.197

7.539 ± 1.124

5.722 ± 0.887

6.176 ± 1.214

1.453 ± 0.266

3.633 ± 0.481
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski