Clostridium phage phiCDHM19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Lubbockvirus; Clostridioides virus phiCDHM19

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090EUD0|A0A090EUD0_9CAUD Uncharacterized protein OS=Clostridium phage phiCDHM19 OX=1522092 GN=phiCDHM19_gp17 PE=4 SV=1
MM1 pKa = 7.37TEE3 pKa = 3.98ADD5 pKa = 4.29ILALTYY11 pKa = 9.75FCKK14 pKa = 8.47MTIRR18 pKa = 11.84RR19 pKa = 11.84CVSIKK24 pKa = 11.01NEE26 pKa = 4.21DD27 pKa = 3.39TGVTYY32 pKa = 10.67FNEE35 pKa = 4.05NVVIAEE41 pKa = 4.53DD42 pKa = 4.48VPCGLNGNIPNVIDD56 pKa = 3.59TDD58 pKa = 3.57ITNSISVFEE67 pKa = 4.51LYY69 pKa = 10.24CRR71 pKa = 11.84PEE73 pKa = 4.29IDD75 pKa = 4.13LQVGDD80 pKa = 4.57ILDD83 pKa = 3.55ITLEE87 pKa = 4.16NGNVEE92 pKa = 4.16TFIASKK98 pKa = 9.57PFPYY102 pKa = 10.04SSHH105 pKa = 6.25LQVNLTLKK113 pKa = 10.13EE114 pKa = 4.08RR115 pKa = 11.84YY116 pKa = 9.07

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090D832|A0A090D832_9CAUD Isoform of A0A090DBR2 Putative tail tape measure protein (Partial) (Fragment) OS=Clostridium phage phiCDHM19 OX=1522092 GN=phiCDHM19_gp18 PE=4 SV=1
MM1 pKa = 7.34SAGSRR6 pKa = 11.84ALEE9 pKa = 3.61AVIRR13 pKa = 11.84MRR15 pKa = 11.84DD16 pKa = 3.3EE17 pKa = 4.01ASRR20 pKa = 11.84TLRR23 pKa = 11.84QVRR26 pKa = 11.84DD27 pKa = 3.55ATRR30 pKa = 11.84ALQNQTNSTSQAQEE44 pKa = 3.61RR45 pKa = 11.84LQEE48 pKa = 4.02QFRR51 pKa = 11.84KK52 pKa = 10.09VSNAAKK58 pKa = 9.93IAGAGIVTGIGAGLVSASKK77 pKa = 10.72AGAEE81 pKa = 4.6FEE83 pKa = 4.4TAMTKK88 pKa = 10.52

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

1

88

15648

34

799

177.8

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.601 ± 0.266

1.412 ± 0.137

5.796 ± 0.254

8.704 ± 0.454

4.167 ± 0.188

5.234 ± 0.491

1.106 ± 0.115

9.119 ± 0.32

10.647 ± 0.381

8.627 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.133

7.304 ± 0.203

1.911 ± 0.111

2.71 ± 0.15

3.662 ± 0.241

6.084 ± 0.194

5.707 ± 0.287

5.803 ± 0.239

0.959 ± 0.128

4.192 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski