Bat coronavirus HKU9-4

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Nobecovirus; Rousettus bat coronavirus HKU9

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3EXJ3|A3EXJ3_BCHK9 Membrane protein OS=Bat coronavirus HKU9-4 OX=424370 GN=M PE=3 SV=1
MM1 pKa = 7.32YY2 pKa = 9.76EE3 pKa = 4.44LVSADD8 pKa = 3.47TSVVIANVLVIIILCLFVVIVGCALLLILQFILGTCGCLFSVICKK53 pKa = 8.12PTILVYY59 pKa = 11.09NKK61 pKa = 9.81FKK63 pKa = 11.29NEE65 pKa = 3.87SLLNEE70 pKa = 4.09QEE72 pKa = 4.15EE73 pKa = 4.57LLVV76 pKa = 3.8

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3EXJ5|A3EXJ5_BCHK9 Uncharacterized protein NS7a OS=Bat coronavirus HKU9-4 OX=424370 GN=NS7a PE=4 SV=1
MM1 pKa = 7.62SGRR4 pKa = 11.84NKK6 pKa = 9.51PRR8 pKa = 11.84PGSQSPKK15 pKa = 8.6VTFKK19 pKa = 11.01QEE21 pKa = 3.89SDD23 pKa = 3.52GSDD26 pKa = 3.37SEE28 pKa = 4.52SEE30 pKa = 3.89RR31 pKa = 11.84RR32 pKa = 11.84NGNRR36 pKa = 11.84SGARR40 pKa = 11.84PKK42 pKa = 10.88NNNNRR47 pKa = 11.84GGNSKK52 pKa = 10.38PEE54 pKa = 4.09KK55 pKa = 9.82PKK57 pKa = 10.53AAPPQNVSWFAPLVQTGKK75 pKa = 10.3QDD77 pKa = 3.28LRR79 pKa = 11.84FPRR82 pKa = 11.84GEE84 pKa = 4.24GVPISQGVDD93 pKa = 3.01PVYY96 pKa = 11.0LHH98 pKa = 6.8GYY100 pKa = 6.03WVRR103 pKa = 11.84TQRR106 pKa = 11.84NFQKK110 pKa = 10.75GGKK113 pKa = 6.6TVQANPRR120 pKa = 11.84WYY122 pKa = 10.03FYY124 pKa = 9.31YY125 pKa = 10.37TGTGRR130 pKa = 11.84HH131 pKa = 6.14ADD133 pKa = 3.75LRR135 pKa = 11.84WGQKK139 pKa = 10.17NPDD142 pKa = 4.47LIWVGEE148 pKa = 4.16DD149 pKa = 3.05GANINRR155 pKa = 11.84IGDD158 pKa = 3.47MGTRR162 pKa = 11.84NPNNDD167 pKa = 2.78SAIPVQFGSGIPKK180 pKa = 10.05GFYY183 pKa = 10.47AEE185 pKa = 4.29GRR187 pKa = 11.84NSRR190 pKa = 11.84GNSRR194 pKa = 11.84NSSRR198 pKa = 11.84NSSRR202 pKa = 11.84ASTRR206 pKa = 11.84GNSRR210 pKa = 11.84ANSRR214 pKa = 11.84GASPGRR220 pKa = 11.84NTPSGGSNSEE230 pKa = 3.14PWMAYY235 pKa = 9.67LVQKK239 pKa = 11.06LEE241 pKa = 4.02ALEE244 pKa = 4.26TKK246 pKa = 10.77VNGNKK251 pKa = 9.83SEE253 pKa = 4.25TKK255 pKa = 10.71APVQVTKK262 pKa = 10.97NAAAEE267 pKa = 3.96NAKK270 pKa = 10.26KK271 pKa = 10.1LRR273 pKa = 11.84HH274 pKa = 5.99KK275 pKa = 8.81RR276 pKa = 11.84TPHH279 pKa = 6.14KK280 pKa = 10.82GSGVTANFGRR290 pKa = 11.84RR291 pKa = 11.84GPGEE295 pKa = 3.93LEE297 pKa = 4.13GNFGDD302 pKa = 5.97LEE304 pKa = 4.15MLKK307 pKa = 10.67LGADD311 pKa = 3.89DD312 pKa = 4.27PRR314 pKa = 11.84FPAVAQMAPNVAAFMFMSHH333 pKa = 6.92FSTRR337 pKa = 11.84DD338 pKa = 3.19EE339 pKa = 5.27EE340 pKa = 4.27DD341 pKa = 3.94ALWLNYY347 pKa = 9.88RR348 pKa = 11.84GAIKK352 pKa = 10.43LPKK355 pKa = 9.56SDD357 pKa = 4.52PNFEE361 pKa = 3.93QWTKK365 pKa = 11.05LLEE368 pKa = 4.49EE369 pKa = 4.59NLNAYY374 pKa = 7.5KK375 pKa = 10.13TFPPPAPKK383 pKa = 9.39KK384 pKa = 9.89DD385 pKa = 3.35KK386 pKa = 10.55KK387 pKa = 10.65KK388 pKa = 10.77KK389 pKa = 9.84EE390 pKa = 3.91EE391 pKa = 4.04ASQEE395 pKa = 3.67IAIFEE400 pKa = 4.41DD401 pKa = 4.01ASTGTDD407 pKa = 3.36QQIVKK412 pKa = 9.86VWVKK416 pKa = 11.32DD417 pKa = 3.54EE418 pKa = 4.17GAQTDD423 pKa = 4.21EE424 pKa = 4.23EE425 pKa = 4.73WLGGDD430 pKa = 3.14DD431 pKa = 3.39TVYY434 pKa = 10.84EE435 pKa = 4.38EE436 pKa = 4.62EE437 pKa = 4.59DD438 pKa = 4.11DD439 pKa = 3.96RR440 pKa = 11.84PKK442 pKa = 9.38TQRR445 pKa = 11.84RR446 pKa = 11.84HH447 pKa = 5.15KK448 pKa = 10.06KK449 pKa = 9.76RR450 pKa = 11.84NSTASRR456 pKa = 11.84VTIADD461 pKa = 3.43PMNATSEE468 pKa = 4.26RR469 pKa = 11.84SS470 pKa = 3.23

Molecular weight: 52.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

9540

76

6925

1192.5

132.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.505 ± 0.313

3.092 ± 0.342

5.273 ± 0.781

3.899 ± 0.343

4.486 ± 0.389

6.31 ± 0.402

1.824 ± 0.281

4.329 ± 0.655

4.801 ± 0.661

9.549 ± 0.724

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.364

5.042 ± 0.848

4.675 ± 0.334

3.365 ± 0.388

4.308 ± 0.511

6.813 ± 0.52

6.52 ± 0.342

9.696 ± 0.797

1.31 ± 0.28

4.79 ± 0.553
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski