Ruminococcaceae bacterium FB2012

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6D798|A0A1G6D798_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium FB2012 OX=1520817 GN=SAMN02910317_02053 PE=4 SV=1
MM1 pKa = 7.22TKK3 pKa = 10.06KK4 pKa = 10.2EE5 pKa = 3.86QLIQKK10 pKa = 10.09LNDD13 pKa = 3.03SYY15 pKa = 11.96AEE17 pKa = 3.98HH18 pKa = 6.92RR19 pKa = 11.84EE20 pKa = 4.1SWLHH24 pKa = 5.77MKK26 pKa = 10.63PEE28 pKa = 4.42DD29 pKa = 4.35LVLLASNIAASDD41 pKa = 3.86YY42 pKa = 11.29AVRR45 pKa = 11.84CVPDD49 pKa = 4.08VIQEE53 pKa = 4.17DD54 pKa = 3.82EE55 pKa = 4.64AEE57 pKa = 4.1YY58 pKa = 11.07LLQFKK63 pKa = 10.8DD64 pKa = 3.7PLNVLSDD71 pKa = 4.39MISEE75 pKa = 4.51SLDD78 pKa = 3.4EE79 pKa = 4.11QYY81 pKa = 11.46GYY83 pKa = 11.33YY84 pKa = 10.48GDD86 pKa = 4.26SLSDD90 pKa = 3.59LLYY93 pKa = 11.08EE94 pKa = 5.47LMDD97 pKa = 4.29KK98 pKa = 11.22GDD100 pKa = 3.63ALEE103 pKa = 4.67YY104 pKa = 11.01YY105 pKa = 10.24DD106 pKa = 5.91LEE108 pKa = 4.58PTAEE112 pKa = 4.03TAEE115 pKa = 4.25VDD117 pKa = 3.46MEE119 pKa = 4.3MM120 pKa = 5.13

Molecular weight: 13.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6AUF8|A0A1G6AUF8_9FIRM Phosphate:Na+ symporter OS=Ruminococcaceae bacterium FB2012 OX=1520817 GN=SAMN02910317_00545 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.65QPKK8 pKa = 9.08KK9 pKa = 8.13LQRR12 pKa = 11.84KK13 pKa = 7.95KK14 pKa = 9.48VHH16 pKa = 6.06GFMQRR21 pKa = 11.84MSTRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.43VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84ARR41 pKa = 11.84LSHH44 pKa = 6.55

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3207

0

3207

1098134

39

3595

342.4

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.044 ± 0.047

1.635 ± 0.019

6.309 ± 0.036

7.326 ± 0.044

4.293 ± 0.027

7.258 ± 0.045

1.541 ± 0.017

6.848 ± 0.035

6.703 ± 0.038

8.592 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.023

4.148 ± 0.037

3.442 ± 0.028

2.473 ± 0.019

4.738 ± 0.043

6.614 ± 0.043

5.716 ± 0.046

6.668 ± 0.034

0.861 ± 0.015

4.066 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski