Garciella nitratireducens DSM 15102

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Garciella; Garciella nitratireducens

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4P6R0|A0A1T4P6R0_9FIRM Altronate dehydratase large subunit OS=Garciella nitratireducens DSM 15102 OX=1121911 GN=SAMN02745973_01940 PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.22KK3 pKa = 10.16YY4 pKa = 10.18VCTVCGYY11 pKa = 10.44VYY13 pKa = 10.5NPEE16 pKa = 4.05VGDD19 pKa = 3.96PDD21 pKa = 4.6GGIAPGTAFEE31 pKa = 5.97DD32 pKa = 4.28IPDD35 pKa = 3.65DD36 pKa = 4.04WVCPEE41 pKa = 4.51CGVGKK46 pKa = 10.48DD47 pKa = 3.17MFEE50 pKa = 4.13PQEE53 pKa = 3.89

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4PI05|A0A1T4PI05_9FIRM Uncharacterized protein OS=Garciella nitratireducens DSM 15102 OX=1121911 GN=SAMN02745973_02099 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.09GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTLSGRR28 pKa = 11.84NVLKK32 pKa = 10.58RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.37KK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2103

0

2103

627865

39

2886

298.6

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.708 ± 0.054

0.997 ± 0.021

4.851 ± 0.034

7.671 ± 0.058

4.337 ± 0.047

6.693 ± 0.05

1.708 ± 0.023

10.487 ± 0.062

9.124 ± 0.061

9.274 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.023

5.119 ± 0.038

3.243 ± 0.032

3.663 ± 0.039

3.605 ± 0.04

5.589 ± 0.035

4.679 ± 0.036

6.033 ± 0.044

0.682 ± 0.02

3.853 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski