Escherichia phage OSYSP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus; Escherichia virus OSYSP

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221J830|A0A221J830_9CAUD Ribonucleotide reductase subunit OS=Escherichia phage OSYSP OX=2020879 GN=OSY_001 PE=4 SV=1
MM1 pKa = 7.82SKK3 pKa = 10.23FQEE6 pKa = 4.17LKK8 pKa = 10.63QEE10 pKa = 4.31LEE12 pKa = 4.0EE13 pKa = 4.61CACPDD18 pKa = 3.63CLGVGEE24 pKa = 4.88SSDD27 pKa = 4.42AEE29 pKa = 4.5AGDD32 pKa = 3.97TFCNIWVCPTCKK44 pKa = 9.8GTGINPTCGISLDD57 pKa = 3.73IEE59 pKa = 4.3

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221J865|A0A221J865_9CAUD DNA ligase (NAD(+)) OS=Escherichia phage OSYSP OX=2020879 GN=OSY_063 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.18NAKK8 pKa = 7.53TQAATTVKK16 pKa = 9.78TFPQTEE22 pKa = 4.31ANRR25 pKa = 11.84KK26 pKa = 8.9ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.6PTDD39 pKa = 3.49AQAAQALNRR48 pKa = 11.84PAPIRR53 pKa = 11.84QKK55 pKa = 10.69PKK57 pKa = 10.23AKK59 pKa = 10.11NATRR63 pKa = 11.84SVARR67 pKa = 11.84LVTYY71 pKa = 9.3VQGYY75 pKa = 5.54GHH77 pKa = 7.1KK78 pKa = 9.69SVSVTLSFNAGAEE91 pKa = 4.17LFSRR95 pKa = 11.84NGMAMKK101 pKa = 10.22DD102 pKa = 3.46YY103 pKa = 10.91EE104 pKa = 4.19KK105 pKa = 10.97AVNQKK110 pKa = 9.97RR111 pKa = 11.84KK112 pKa = 8.13PTADD116 pKa = 3.13VLRR119 pKa = 11.84DD120 pKa = 3.4TRR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.85YY137 pKa = 11.02SKK139 pKa = 11.81DD140 pKa = 3.37NVRR143 pKa = 11.84ALCYY147 pKa = 10.2GVGIKK152 pKa = 8.51FTGDD156 pKa = 3.22SARR159 pKa = 11.84KK160 pKa = 7.85SAKK163 pKa = 9.24PARR166 pKa = 11.84KK167 pKa = 9.36RR168 pKa = 11.84KK169 pKa = 9.78AKK171 pKa = 10.37

Molecular weight: 18.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

30998

27

1227

186.7

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.755 ± 0.414

1.119 ± 0.094

6.149 ± 0.149

6.978 ± 0.199

4.145 ± 0.138

6.355 ± 0.193

1.884 ± 0.121

6.736 ± 0.187

7.278 ± 0.224

8.439 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.149

5.2 ± 0.193

3.391 ± 0.159

3.903 ± 0.241

4.436 ± 0.143

6.229 ± 0.207

5.765 ± 0.166

6.446 ± 0.175

1.219 ± 0.079

3.871 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski