Gordonia phage Lozinak

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Smoothievirus; unclassified Smoothievirus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GGA1|A0A2D1GGA1_9CAUD Uncharacterized protein OS=Gordonia phage Lozinak OX=2041513 GN=SEA_LOZINAK_171 PE=4 SV=1
MM1 pKa = 6.93STIDD5 pKa = 3.04ITTQVLAEE13 pKa = 4.16MWTEE17 pKa = 3.46NTGRR21 pKa = 11.84HH22 pKa = 5.21FLDD25 pKa = 3.38SGGAYY30 pKa = 8.98GRR32 pKa = 11.84SWEE35 pKa = 4.14RR36 pKa = 11.84NQAATAGVDD45 pKa = 2.9AGEE48 pKa = 4.53YY49 pKa = 10.13FLSLPQVTLDD59 pKa = 3.2ASYY62 pKa = 10.98NYY64 pKa = 10.46VDD66 pKa = 3.23ITVSAFHH73 pKa = 7.05WLNEE77 pKa = 3.91ILDD80 pKa = 3.82YY81 pKa = 11.08APRR84 pKa = 11.84LNRR87 pKa = 11.84MFEE90 pKa = 4.05MFVALEE96 pKa = 4.25DD97 pKa = 3.76EE98 pKa = 4.84RR99 pKa = 11.84DD100 pKa = 3.98RR101 pKa = 11.84PWMAEE106 pKa = 3.35AEE108 pKa = 4.45AFVEE112 pKa = 4.03WLEE115 pKa = 4.05QHH117 pKa = 6.12GRR119 pKa = 11.84ASDD122 pKa = 3.62VQTVNTYY129 pKa = 10.71NGEE132 pKa = 4.05TWLDD136 pKa = 3.25STLQYY141 pKa = 11.59VVFTYY146 pKa = 9.98TDD148 pKa = 3.34RR149 pKa = 11.84DD150 pKa = 3.81GFDD153 pKa = 3.34TDD155 pKa = 3.79MVMLQYY161 pKa = 10.73HH162 pKa = 6.75GGCDD166 pKa = 3.31VRR168 pKa = 11.84AGYY171 pKa = 7.45TKK173 pKa = 10.35PRR175 pKa = 11.84AFSIGGYY182 pKa = 7.03EE183 pKa = 4.14GRR185 pKa = 11.84YY186 pKa = 8.37EE187 pKa = 4.74LYY189 pKa = 10.22TEE191 pKa = 4.28GHH193 pKa = 5.94VSLSCTEE200 pKa = 4.27NYY202 pKa = 9.56GQEE205 pKa = 3.94IGVGLDD211 pKa = 2.84GDD213 pKa = 4.03TVYY216 pKa = 11.14AGTHH220 pKa = 5.29AWDD223 pKa = 4.11SNSSGVDD230 pKa = 2.73WVEE233 pKa = 3.45YY234 pKa = 10.26GGAYY238 pKa = 7.82DD239 pKa = 3.86TPDD242 pKa = 3.35FEE244 pKa = 5.08TIEE247 pKa = 4.62PEE249 pKa = 4.52DD250 pKa = 4.37DD251 pKa = 3.28GTPYY255 pKa = 10.07VACPTCKK262 pKa = 10.39APLQVSVHH270 pKa = 6.5FGHH273 pKa = 7.73

Molecular weight: 30.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GFV2|A0A2D1GFV2_9CAUD Uncharacterized protein OS=Gordonia phage Lozinak OX=2041513 GN=SEA_LOZINAK_105 PE=4 SV=1
MM1 pKa = 7.39PRR3 pKa = 11.84SRR5 pKa = 11.84SWSDD9 pKa = 3.05DD10 pKa = 3.38DD11 pKa = 4.9LRR13 pKa = 11.84AAVRR17 pKa = 11.84SSQTYY22 pKa = 10.19ADD24 pKa = 3.69VARR27 pKa = 11.84ALSMHH32 pKa = 6.94PGSSTVGRR40 pKa = 11.84LRR42 pKa = 11.84DD43 pKa = 3.46RR44 pKa = 11.84TRR46 pKa = 11.84ALGLDD51 pKa = 3.31TRR53 pKa = 11.84HH54 pKa = 5.64FTRR57 pKa = 11.84ARR59 pKa = 11.84PRR61 pKa = 11.84EE62 pKa = 3.81DD63 pKa = 2.83MFRR66 pKa = 11.84SGVRR70 pKa = 11.84YY71 pKa = 7.38HH72 pKa = 6.2TGMRR76 pKa = 11.84DD77 pKa = 2.94RR78 pKa = 11.84YY79 pKa = 8.64LQLMPYY85 pKa = 8.75EE86 pKa = 4.24CQICGVSKK94 pKa = 10.15WCGQPLTLQLDD105 pKa = 4.43HH106 pKa = 7.59IDD108 pKa = 3.63GDD110 pKa = 4.0RR111 pKa = 11.84MNNTLQNLRR120 pKa = 11.84LLCPNCHH127 pKa = 6.03SQTATWSRR135 pKa = 11.84KK136 pKa = 8.97KK137 pKa = 10.77SNPVV141 pKa = 2.8

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

179

0

179

29354

27

2931

164.0

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.576 ± 0.33

1.036 ± 0.126

7.137 ± 0.182

6.752 ± 0.206

3.158 ± 0.111

7.856 ± 0.241

2.34 ± 0.146

4.735 ± 0.155

4.085 ± 0.169

7.624 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.099

3.655 ± 0.149

4.889 ± 0.173

3.655 ± 0.217

6.984 ± 0.209

5.73 ± 0.146

5.86 ± 0.188

7.375 ± 0.269

2.146 ± 0.095

2.906 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski