Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Methylibium; Methylibium petroleiphilum

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4359 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2SPB6|A2SPB6_METPP Uncharacterized protein OS=Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1) OX=420662 GN=Mpe_B0641 PE=4 SV=1
MM1 pKa = 7.6IKK3 pKa = 10.26NFKK6 pKa = 9.52KK7 pKa = 8.68WQCVTCSFTYY17 pKa = 10.38DD18 pKa = 3.66EE19 pKa = 4.55EE20 pKa = 6.12LGMPSDD26 pKa = 5.42GIPPGTAWEE35 pKa = 4.61DD36 pKa = 3.76VPDD39 pKa = 4.65DD40 pKa = 4.06WTCPDD45 pKa = 3.75CSSPKK50 pKa = 10.08SDD52 pKa = 3.23FQMVEE57 pKa = 3.51II58 pKa = 5.02

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2SMM9|A2SMM9_METPP Uncharacterized protein OS=Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1) OX=420662 GN=Mpe_B0037 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.29TRR25 pKa = 11.84GGRR28 pKa = 11.84RR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.5GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LGLL44 pKa = 3.7

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4359

0

4359

1382589

29

2092

317.2

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.426 ± 0.059

0.979 ± 0.011

5.361 ± 0.026

5.4 ± 0.029

3.352 ± 0.024

8.44 ± 0.032

2.283 ± 0.018

3.918 ± 0.026

2.931 ± 0.034

10.95 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.018

2.244 ± 0.022

5.517 ± 0.031

3.722 ± 0.018

7.722 ± 0.036

5.243 ± 0.029

5.003 ± 0.026

7.685 ± 0.032

1.518 ± 0.016

2.108 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski