Aminobacterium colombiense (strain DSM 12261 / ALA-1)

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Aminobacterium; Aminobacterium colombiense

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5EFB4|D5EFB4_AMICL Uncharacterized protein OS=Aminobacterium colombiense (strain DSM 12261 / ALA-1) OX=572547 GN=Amico_1122 PE=4 SV=1
MM1 pKa = 7.26SAKK4 pKa = 10.24EE5 pKa = 3.73KK6 pKa = 10.28IAYY9 pKa = 9.7LKK11 pKa = 11.03GLLAGLNMKK20 pKa = 10.74DD21 pKa = 3.04EE22 pKa = 4.47TAAKK26 pKa = 9.81AFSSVTEE33 pKa = 4.01ALEE36 pKa = 3.99ALADD40 pKa = 4.53EE41 pKa = 4.9IEE43 pKa = 4.16EE44 pKa = 3.94HH45 pKa = 6.4AALIEE50 pKa = 4.13EE51 pKa = 4.22QQDD54 pKa = 3.82LYY56 pKa = 11.61EE57 pKa = 4.21EE58 pKa = 4.95LEE60 pKa = 4.56DD61 pKa = 5.67DD62 pKa = 5.71FSLLDD67 pKa = 3.85EE68 pKa = 5.59DD69 pKa = 5.99LDD71 pKa = 3.85TLEE74 pKa = 4.76RR75 pKa = 11.84SFAEE79 pKa = 4.53FMGEE83 pKa = 4.06DD84 pKa = 3.64FEE86 pKa = 4.68EE87 pKa = 4.35TDD89 pKa = 3.44EE90 pKa = 4.53EE91 pKa = 4.33EE92 pKa = 4.57NEE94 pKa = 3.63FDD96 pKa = 3.39EE97 pKa = 5.92AYY99 pKa = 10.46EE100 pKa = 4.24SVACPKK106 pKa = 9.92CGHH109 pKa = 5.43VFYY112 pKa = 9.97YY113 pKa = 9.44QPEE116 pKa = 4.23MYY118 pKa = 10.24EE119 pKa = 3.85EE120 pKa = 5.48GEE122 pKa = 4.08ALQCPDD128 pKa = 3.99CGEE131 pKa = 4.41TFAQPAEE138 pKa = 4.24EE139 pKa = 4.01

Molecular weight: 15.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5EG22|D5EG22_AMICL Epoxyqueuosine reductase QueH OS=Aminobacterium colombiense (strain DSM 12261 / ALA-1) OX=572547 GN=queH PE=3 SV=1
MM1 pKa = 7.03TAPGRR6 pKa = 11.84MPALFSLAGICLALLVLQQGIPLFIFPLVAMSGTLALVLLFTTQEE51 pKa = 3.93KK52 pKa = 8.8PGYY55 pKa = 7.9WKK57 pKa = 10.81GVTLVVLVAGVGSAYY72 pKa = 9.71ISSRR76 pKa = 11.84LSTDD80 pKa = 3.57FLHH83 pKa = 7.18PGEE86 pKa = 4.38TFQGQGYY93 pKa = 9.65ILLEE97 pKa = 4.33RR98 pKa = 11.84PWGRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84ALLIRR109 pKa = 11.84TKK111 pKa = 10.63KK112 pKa = 9.5GTFLAKK118 pKa = 9.99IPSQHH123 pKa = 5.82GFIAGDD129 pKa = 3.7TVEE132 pKa = 4.29VAGTMLSLPSSKK144 pKa = 10.19KK145 pKa = 9.79SRR147 pKa = 11.84SFHH150 pKa = 6.26EE151 pKa = 3.8DD152 pKa = 3.43RR153 pKa = 11.84YY154 pKa = 8.72WKK156 pKa = 10.67ARR158 pKa = 11.84GAMAEE163 pKa = 3.92IVIGKK168 pKa = 7.88VEE170 pKa = 4.47KK171 pKa = 10.5IEE173 pKa = 4.22SCRR176 pKa = 11.84QFSLSGWRR184 pKa = 11.84NMLVKK189 pKa = 10.47RR190 pKa = 11.84ILLTQPPRR198 pKa = 11.84TRR200 pKa = 11.84GYY202 pKa = 10.52LAAAWTGMKK211 pKa = 10.25SPLLSSQHH219 pKa = 5.4EE220 pKa = 4.27RR221 pKa = 11.84WGTSHH226 pKa = 7.42LLAVSGFHH234 pKa = 6.57VSLFVSGLLFLLRR247 pKa = 11.84SCLGRR252 pKa = 11.84GFIISWAMWGYY263 pKa = 10.89VLIAGAAPSALRR275 pKa = 11.84AALMMQIALIGRR287 pKa = 11.84CRR289 pKa = 11.84GRR291 pKa = 11.84PSQAVNTVSLAGMALLFWRR310 pKa = 11.84PWWFWDD316 pKa = 4.85LGWQLSMVAALIIAAALEE334 pKa = 4.58RR335 pKa = 11.84GLSPVWWLSVGPAIWVTTLPLAAPIFGDD363 pKa = 3.68VPLAGIVTNLSAGPVFGVLYY383 pKa = 9.92PLASFLALPSLLALPWGNWASQGAEE408 pKa = 3.7GLFILWEE415 pKa = 4.12TWADD419 pKa = 3.77SISRR423 pKa = 11.84LIPVYY428 pKa = 10.73VPWTPFWTAFAAGLLPLLIARR449 pKa = 11.84SLRR452 pKa = 11.84LSWRR456 pKa = 11.84HH457 pKa = 5.47SLAASLLLQCVMLWIVMM474 pKa = 4.14

Molecular weight: 51.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1872

0

1872

596225

31

1759

318.5

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.204 ± 0.051

1.227 ± 0.021

4.763 ± 0.041

7.142 ± 0.063

4.3 ± 0.04

7.837 ± 0.042

1.933 ± 0.026

7.109 ± 0.047

5.722 ± 0.048

10.409 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.024

3.321 ± 0.028

4.307 ± 0.034

2.94 ± 0.027

5.325 ± 0.046

6.249 ± 0.039

4.799 ± 0.031

7.447 ± 0.056

1.278 ± 0.023

2.918 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski