Staphylococcus phage vB_SepS_SEP9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sextaecvirus; Staphylococcus virus SEP9

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5RVA7|W5RVA7_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SepS_SEP9 OX=1434319 GN=SEP9_109 PE=4 SV=1
MM1 pKa = 7.42QEE3 pKa = 4.03LSNVLKK9 pKa = 11.01NMEE12 pKa = 4.58LEE14 pKa = 4.22THH16 pKa = 5.76GVKK19 pKa = 10.38NDD21 pKa = 3.54IINYY25 pKa = 9.34LLNNSNDD32 pKa = 3.6SEE34 pKa = 4.84DD35 pKa = 3.61IQILLEE41 pKa = 4.85DD42 pKa = 3.76INNYY46 pKa = 9.33GCEE49 pKa = 4.02SGIVNHH55 pKa = 7.05LIYY58 pKa = 10.44YY59 pKa = 7.69TDD61 pKa = 3.19TVAYY65 pKa = 9.58FEE67 pKa = 4.17KK68 pKa = 10.83HH69 pKa = 5.12EE70 pKa = 4.85DD71 pKa = 3.88EE72 pKa = 4.18IQEE75 pKa = 4.41MINNTYY81 pKa = 10.73SIDD84 pKa = 3.88LYY86 pKa = 11.65SMGVEE91 pKa = 4.07EE92 pKa = 5.75FDD94 pKa = 4.45KK95 pKa = 11.35LMNILNLDD103 pKa = 3.69SFEE106 pKa = 5.37DD107 pKa = 3.47RR108 pKa = 11.84LNNIGTHH115 pKa = 5.3EE116 pKa = 4.06EE117 pKa = 3.73ALKK120 pKa = 10.67RR121 pKa = 11.84VAYY124 pKa = 10.11DD125 pKa = 3.35NNISIDD131 pKa = 3.53KK132 pKa = 10.65ADD134 pKa = 3.52TKK136 pKa = 10.97DD137 pKa = 3.56YY138 pKa = 10.52EE139 pKa = 4.38EE140 pKa = 4.25EE141 pKa = 4.45VFTEE145 pKa = 4.88RR146 pKa = 11.84YY147 pKa = 9.79YY148 pKa = 10.34IATEE152 pKa = 4.35LGIADD157 pKa = 4.21KK158 pKa = 11.2NYY160 pKa = 9.73LAWAIFEE167 pKa = 4.58YY168 pKa = 10.4NASVLYY174 pKa = 10.77DD175 pKa = 3.68EE176 pKa = 5.58LYY178 pKa = 11.19EE179 pKa = 4.07MSLVV183 pKa = 3.54

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5RV72|W5RV72_9CAUD Ribonuclease H OS=Staphylococcus phage vB_SepS_SEP9 OX=1434319 GN=SEP9_067 PE=3 SV=1
MM1 pKa = 7.59SNTPEE6 pKa = 3.76YY7 pKa = 10.52KK8 pKa = 10.09AWFEE12 pKa = 4.16MKK14 pKa = 10.1RR15 pKa = 11.84RR16 pKa = 11.84CYY18 pKa = 10.08NKK20 pKa = 9.5NRR22 pKa = 11.84KK23 pKa = 8.46GYY25 pKa = 9.83KK26 pKa = 9.26NYY28 pKa = 9.92GGRR31 pKa = 11.84GIKK34 pKa = 10.12VCDD37 pKa = 2.99RR38 pKa = 11.84WLEE41 pKa = 4.13SFEE44 pKa = 4.71NFYY47 pKa = 11.24EE48 pKa = 4.53DD49 pKa = 3.15MGDD52 pKa = 3.43RR53 pKa = 11.84PSPNHH58 pKa = 6.0SLDD61 pKa = 4.35RR62 pKa = 11.84IDD64 pKa = 4.56VNGNYY69 pKa = 9.53EE70 pKa = 3.89PSNCKK75 pKa = 9.66WSDD78 pKa = 3.27RR79 pKa = 11.84TEE81 pKa = 3.68QNYY84 pKa = 8.13NQRR87 pKa = 11.84FRR89 pKa = 11.84GGVTNQPNIKK99 pKa = 9.37TNGHH103 pKa = 4.72TFRR106 pKa = 11.84ARR108 pKa = 11.84VSRR111 pKa = 11.84YY112 pKa = 7.67KK113 pKa = 10.54VSRR116 pKa = 11.84ISYY119 pKa = 8.72SLKK122 pKa = 10.11KK123 pKa = 9.86HH124 pKa = 5.56

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

26946

28

2395

208.9

24.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.461 ± 0.435

0.79 ± 0.109

6.825 ± 0.269

7.838 ± 0.417

4.004 ± 0.173

5.897 ± 0.457

1.666 ± 0.105

7.979 ± 0.325

9.805 ± 0.278

8.031 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.135

7.151 ± 0.254

2.245 ± 0.116

3.251 ± 0.172

3.826 ± 0.158

6.175 ± 0.225

5.374 ± 0.17

5.96 ± 0.179

1.225 ± 0.167

4.902 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski