Escherichia phage J8-65

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bonnellvirus; Escherichia virus J8-65

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088F6I1|A0A088F6I1_9CAUD Tail protein B OS=Escherichia phage J8-65 OX=1536597 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 9.11LTVVLLNNLPASAFGEE19 pKa = 4.74GVKK22 pKa = 10.86LEE24 pKa = 4.38DD25 pKa = 3.85VQSKK29 pKa = 10.0QYY31 pKa = 11.13VAITDD36 pKa = 3.75GQGYY40 pKa = 9.81LIAGINKK47 pKa = 10.28DD48 pKa = 3.41GDD50 pKa = 3.88SKK52 pKa = 11.76EE53 pKa = 4.07EE54 pKa = 3.82IEE56 pKa = 4.36AHH58 pKa = 5.99FRR60 pKa = 11.84EE61 pKa = 4.36QGLDD65 pKa = 2.92ADD67 pKa = 4.45MPLALIAMLNGVPFVDD83 pKa = 3.71AADD86 pKa = 3.71VVGADD91 pKa = 2.97IDD93 pKa = 3.84EE94 pKa = 4.68VYY96 pKa = 10.8SVEE99 pKa = 4.86SDD101 pKa = 4.02DD102 pKa = 4.22EE103 pKa = 4.27

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088F6I0|A0A088F6I0_9CAUD Uncharacterized protein OS=Escherichia phage J8-65 OX=1536597 PE=4 SV=1
MM1 pKa = 7.38GSVGKK6 pKa = 9.72VFKK9 pKa = 11.07GIGSVFGLGSQTPKK23 pKa = 9.87YY24 pKa = 8.75TIKK27 pKa = 9.98TDD29 pKa = 3.8PNQVRR34 pKa = 11.84AEE36 pKa = 4.13TMNANLQADD45 pKa = 4.83LSNDD49 pKa = 3.34NTPTIQSGGQVSSASDD65 pKa = 3.31TLSGRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84TGTGVASNLGIGSS86 pKa = 3.69

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12732

60

1033

270.9

29.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.328 ± 0.571

0.966 ± 0.179

5.993 ± 0.284

6.221 ± 0.461

3.424 ± 0.192

7.736 ± 0.295

1.854 ± 0.224

4.705 ± 0.234

4.658 ± 0.384

8.027 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.249

4.398 ± 0.387

4.249 ± 0.372

4.995 ± 0.59

5.584 ± 0.255

5.686 ± 0.374

6.15 ± 0.464

7.061 ± 0.305

1.422 ± 0.16

3.676 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski