Paenibacillus phage PG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9TLQ8|R9TLQ8_9CAUD Uncharacterized protein OS=Paenibacillus phage PG1 OX=754053 GN=PANG_00049 PE=4 SV=1
MM1 pKa = 7.11NVKK4 pKa = 9.97EE5 pKa = 4.28LIEE8 pKa = 4.3RR9 pKa = 11.84LSGYY13 pKa = 10.51DD14 pKa = 4.13DD15 pKa = 4.55EE16 pKa = 6.65IEE18 pKa = 4.27VKK20 pKa = 10.54FGIWNPEE27 pKa = 3.51TCEE30 pKa = 4.09AEE32 pKa = 4.04EE33 pKa = 4.18TFPINDD39 pKa = 3.56VNPYY43 pKa = 10.8DD44 pKa = 3.81YY45 pKa = 10.87LDD47 pKa = 3.83PVLYY51 pKa = 9.86IWSEE55 pKa = 3.85

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TQ42|R9TQ42_9CAUD Uncharacterized protein OS=Paenibacillus phage PG1 OX=754053 GN=PANG_00063 PE=4 SV=1
MM1 pKa = 7.4IKK3 pKa = 10.42KK4 pKa = 9.24LICALIGHH12 pKa = 7.29RR13 pKa = 11.84WAQHH17 pKa = 5.31TYY19 pKa = 9.37VQGNTYY25 pKa = 8.75TGKK28 pKa = 9.84HH29 pKa = 5.14CWRR32 pKa = 11.84CKK34 pKa = 10.26KK35 pKa = 10.33GLPANAHH42 pKa = 6.38IYY44 pKa = 9.62MEE46 pKa = 3.73IRR48 pKa = 11.84RR49 pKa = 11.84NKK51 pKa = 10.4DD52 pKa = 3.2HH53 pKa = 7.29INLPGEE59 pKa = 4.21EE60 pKa = 4.24TTVV63 pKa = 3.01

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

10597

49

617

158.2

17.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.983 ± 0.387

0.934 ± 0.147

6.209 ± 0.235

8.314 ± 0.499

3.718 ± 0.249

6.936 ± 0.341

1.689 ± 0.208

7.002 ± 0.314

7.455 ± 0.368

7.757 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.182

5.294 ± 0.272

3.246 ± 0.192

3.907 ± 0.263

4.662 ± 0.278

5.643 ± 0.249

5.407 ± 0.342

6.568 ± 0.27

1.529 ± 0.164

3.945 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski