Cucurbit aphid-borne yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65970|Q65970_9LUTE Coat protein OS=Cucurbit aphid-borne yellows virus OX=91753 PE=4 SV=1
MM1 pKa = 7.65APTYY5 pKa = 10.11FAFFLLVCLCSSVSSYY21 pKa = 10.8QGTMFIPLEE30 pKa = 4.16PANASYY36 pKa = 10.32WLDD39 pKa = 3.5STDD42 pKa = 3.09IAVPPSHH49 pKa = 6.92PQVQLIYY56 pKa = 10.38DD57 pKa = 4.44CPPQKK62 pKa = 9.89MLRR65 pKa = 11.84DD66 pKa = 4.32FSSRR70 pKa = 11.84DD71 pKa = 2.89ITLEE75 pKa = 3.56LWEE78 pKa = 4.46RR79 pKa = 11.84GYY81 pKa = 11.46NEE83 pKa = 3.54TRR85 pKa = 11.84QAFSEE90 pKa = 4.3AMQNLQNLLMSGVRR104 pKa = 11.84QFHH107 pKa = 6.35TGLEE111 pKa = 4.29SLLHH115 pKa = 6.44VILQTAAYY123 pKa = 9.07LWTSLIWATACATWYY138 pKa = 10.6LLSKK142 pKa = 9.09YY143 pKa = 8.12TIEE146 pKa = 4.12MLTLASLYY154 pKa = 10.09ISTVYY159 pKa = 8.23MVKK162 pKa = 9.17MAAWIFGDD170 pKa = 4.92LPISLLKK177 pKa = 10.8AGLSMVRR184 pKa = 11.84GVSRR188 pKa = 11.84ALWYY192 pKa = 10.11KK193 pKa = 10.38RR194 pKa = 11.84SYY196 pKa = 10.09NAEE199 pKa = 4.01KK200 pKa = 10.58SVEE203 pKa = 4.2GYY205 pKa = 11.01LSFKK209 pKa = 10.49IPQNPPKK216 pKa = 10.74NSVLQVQYY224 pKa = 11.22KK225 pKa = 9.94DD226 pKa = 3.8GSHH229 pKa = 6.69AGYY232 pKa = 8.07ATCVTLYY239 pKa = 10.85NGTNGLLTAYY249 pKa = 9.58HH250 pKa = 5.73VAVPGSKK257 pKa = 10.08VVSTRR262 pKa = 11.84NGNKK266 pKa = 9.2VPLSEE271 pKa = 4.51FRR273 pKa = 11.84SIMEE277 pKa = 3.91SEE279 pKa = 4.06KK280 pKa = 10.67RR281 pKa = 11.84DD282 pKa = 3.77LVLLAGPPNWEE293 pKa = 3.62GTLACKK299 pKa = 10.06AVQFQSAQNLCKK311 pKa = 10.58SKK313 pKa = 11.09ASFYY317 pKa = 10.84AYY319 pKa = 10.15DD320 pKa = 3.74GEE322 pKa = 4.67GWISSNAEE330 pKa = 3.36IVGIAEE336 pKa = 4.61GKK338 pKa = 7.08THH340 pKa = 7.5ASVLSNTDD348 pKa = 3.14AGHH351 pKa = 6.74SGTPYY356 pKa = 9.83FNGRR360 pKa = 11.84TVLGVHH366 pKa = 6.02VGGAKK371 pKa = 10.31DD372 pKa = 3.51EE373 pKa = 4.43NFNYY377 pKa = 7.68MAPIPPVYY385 pKa = 10.58GLTSPSYY392 pKa = 10.18EE393 pKa = 4.28FEE395 pKa = 3.95TTAPQGRR402 pKa = 11.84LFTQEE407 pKa = 4.23EE408 pKa = 4.28IEE410 pKa = 4.08EE411 pKa = 4.74LIEE414 pKa = 4.0EE415 pKa = 4.57FSFSEE420 pKa = 3.77ITSIMGHH427 pKa = 5.39RR428 pKa = 11.84RR429 pKa = 11.84FHH431 pKa = 6.85QMHH434 pKa = 7.39DD435 pKa = 4.0SQRR438 pKa = 11.84HH439 pKa = 3.72QADD442 pKa = 3.47YY443 pKa = 10.41EE444 pKa = 4.34YY445 pKa = 10.89EE446 pKa = 4.05SGKK449 pKa = 10.33RR450 pKa = 11.84AGGGDD455 pKa = 2.9RR456 pKa = 11.84RR457 pKa = 11.84NNRR460 pKa = 11.84TRR462 pKa = 11.84CHH464 pKa = 5.61PRR466 pKa = 11.84GWAHH470 pKa = 6.14KK471 pKa = 7.71WRR473 pKa = 11.84RR474 pKa = 11.84TVSCCSLFPEE484 pKa = 5.11GQHH487 pKa = 5.2VHH489 pKa = 5.55TSEE492 pKa = 4.13QTGKK496 pKa = 8.44PHH498 pKa = 6.84CFCSIPHH505 pKa = 5.68HH506 pKa = 5.36QCFYY510 pKa = 10.56RR511 pKa = 11.84HH512 pKa = 5.61FVGNQTGNNGQNRR525 pKa = 11.84CPFNRR530 pKa = 11.84EE531 pKa = 3.25ASGPSLGGQSHH542 pKa = 6.87EE543 pKa = 4.34EE544 pKa = 4.12TEE546 pKa = 4.6VAQTRR551 pKa = 11.84QEE553 pKa = 4.11KK554 pKa = 10.41FEE556 pKa = 4.07EE557 pKa = 4.35LATDD561 pKa = 4.34FSSFFDD567 pKa = 3.83AQYY570 pKa = 9.27TWEE573 pKa = 4.22QGGEE577 pKa = 3.98EE578 pKa = 4.35APGFDD583 pKa = 4.49KK584 pKa = 11.31VGSLPQFYY592 pKa = 9.77HH593 pKa = 7.1AKK595 pKa = 9.43QKK597 pKa = 10.8KK598 pKa = 8.26SSNWGDD604 pKa = 4.07KK605 pKa = 9.79ICKK608 pKa = 7.79QHH610 pKa = 7.24PEE612 pKa = 4.09MGDD615 pKa = 3.31LTKK618 pKa = 11.17GFGWPQFGAKK628 pKa = 10.1AEE630 pKa = 4.19LKK632 pKa = 10.47SLRR635 pKa = 11.84LQAARR640 pKa = 11.84WLEE643 pKa = 3.8RR644 pKa = 11.84AQSVKK649 pKa = 10.18IPSSEE654 pKa = 3.78EE655 pKa = 3.63RR656 pKa = 11.84EE657 pKa = 4.21HH658 pKa = 8.71VIEE661 pKa = 4.38RR662 pKa = 11.84CCRR665 pKa = 11.84AYY667 pKa = 10.26QAAKK671 pKa = 9.66TNGPMATRR679 pKa = 11.84GDD681 pKa = 3.71RR682 pKa = 11.84LSWDD686 pKa = 3.35NFLQDD691 pKa = 3.64FKK693 pKa = 11.4QAVLSLEE700 pKa = 3.82MDD702 pKa = 3.6AGIGVPYY709 pKa = 9.92IAYY712 pKa = 8.45GKK714 pKa = 6.46PTHH717 pKa = 6.59RR718 pKa = 11.84GWVEE722 pKa = 3.91DD723 pKa = 3.97KK724 pKa = 11.04KK725 pKa = 11.11LLPILARR732 pKa = 11.84LTFNRR737 pKa = 11.84LQKK740 pKa = 9.25MLEE743 pKa = 3.77VRR745 pKa = 11.84YY746 pKa = 10.3VDD748 pKa = 4.19LSPAEE753 pKa = 3.89LVRR756 pKa = 11.84RR757 pKa = 11.84GLCDD761 pKa = 4.7PIRR764 pKa = 11.84VFVKK768 pKa = 10.82GEE770 pKa = 3.63PHH772 pKa = 6.0KK773 pKa = 10.35QAKK776 pKa = 9.74LDD778 pKa = 3.49EE779 pKa = 4.35GRR781 pKa = 11.84YY782 pKa = 8.74RR783 pKa = 11.84LIMSVSLVDD792 pKa = 3.45QLVARR797 pKa = 11.84VLFQNQNKK805 pKa = 9.66RR806 pKa = 11.84EE807 pKa = 3.81IALWRR812 pKa = 11.84VVPSKK817 pKa = 10.72PGFGLSTDD825 pKa = 3.58EE826 pKa = 4.04QVAEE830 pKa = 4.3FMQILSAQVGLTPSDD845 pKa = 5.62LITEE849 pKa = 4.69WRR851 pKa = 11.84AHH853 pKa = 6.44MIATDD858 pKa = 3.65CSGFDD863 pKa = 3.32WSVSDD868 pKa = 4.58WLLEE872 pKa = 4.07DD873 pKa = 4.28DD874 pKa = 3.82MEE876 pKa = 4.4VRR878 pKa = 11.84NRR880 pKa = 11.84LTLDD884 pKa = 3.27LNEE887 pKa = 3.95TTRR890 pKa = 11.84RR891 pKa = 11.84LRR893 pKa = 11.84SWLYY897 pKa = 10.5CISNSVLCLSDD908 pKa = 3.31GTLLAQRR915 pKa = 11.84VPGVQKK921 pKa = 10.45SGSYY925 pKa = 8.25NTSSSNSRR933 pKa = 11.84IRR935 pKa = 11.84VMAAYY940 pKa = 9.93HH941 pKa = 6.65CGAEE945 pKa = 3.89WAMAMGDD952 pKa = 3.92DD953 pKa = 4.41ALEE956 pKa = 4.35SACSNLEE963 pKa = 3.95RR964 pKa = 11.84YY965 pKa = 9.28KK966 pKa = 10.97SLGFKK971 pKa = 10.48VEE973 pKa = 4.14EE974 pKa = 4.08SSKK977 pKa = 11.47LEE979 pKa = 3.98FCSHH983 pKa = 6.1IFEE986 pKa = 5.2KK987 pKa = 10.64EE988 pKa = 3.74DD989 pKa = 3.82LAIPVNKK996 pKa = 10.52AKK998 pKa = 9.87MLYY1001 pKa = 10.13KK1002 pKa = 10.31LIHH1005 pKa = 6.42GYY1007 pKa = 9.43EE1008 pKa = 4.18PEE1010 pKa = 4.59CGNVEE1015 pKa = 4.12VLINYY1020 pKa = 7.74LAACFSILNEE1030 pKa = 3.97LRR1032 pKa = 11.84SDD1034 pKa = 3.67PSLVEE1039 pKa = 4.43TLHH1042 pKa = 5.54QWLVAPVQPQKK1053 pKa = 10.84II1054 pKa = 3.87

Molecular weight: 118.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65968|Q65968_9LUTE Serine protease OS=Cucurbit aphid-borne yellows virus OX=91753 PE=4 SV=1
MM1 pKa = 7.29QIEE4 pKa = 4.56SVKK7 pKa = 10.07QQLIFRR13 pKa = 11.84PTRR16 pKa = 11.84RR17 pKa = 11.84TSINDD22 pKa = 3.15RR23 pKa = 11.84KK24 pKa = 10.88LNVANFLINHH34 pKa = 5.98SFFLAINGTNLLRR47 pKa = 11.84LFLARR52 pKa = 11.84LPLLISEE59 pKa = 4.56QLSGDD64 pKa = 3.65YY65 pKa = 10.69VYY67 pKa = 10.95TPGASKK73 pKa = 10.7RR74 pKa = 11.84IILARR79 pKa = 11.84FHH81 pKa = 6.21RR82 pKa = 11.84HH83 pKa = 5.68CGAPLPSSSAVDD95 pKa = 3.7LRR97 pKa = 11.84LPASKK102 pKa = 10.55DD103 pKa = 3.19VARR106 pKa = 11.84FFLARR111 pKa = 11.84HH112 pKa = 5.27YY113 pKa = 11.19SRR115 pKa = 11.84ALGEE119 pKa = 4.17RR120 pKa = 11.84IQRR123 pKa = 11.84NQTSLFRR130 pKa = 11.84GYY132 pKa = 11.46AEE134 pKa = 3.67FAKK137 pKa = 10.27FINVWCSSISHH148 pKa = 6.93RR149 pKa = 11.84LGEE152 pKa = 3.99FTPRR156 pKa = 11.84NFTNGSIFVDD166 pKa = 4.63LSNLGHH172 pKa = 5.94SLCDD176 pKa = 3.28LVLAQQVHH184 pKa = 6.8DD185 pKa = 3.86RR186 pKa = 11.84DD187 pKa = 3.81AYY189 pKa = 9.56TRR191 pKa = 11.84LALHH195 pKa = 6.09IHH197 pKa = 7.1RR198 pKa = 11.84IYY200 pKa = 11.26GEE202 pKa = 4.34DD203 pKa = 3.29GGLDD207 pKa = 3.71FWRR210 pKa = 11.84LANFPSKK217 pKa = 10.14SWPFNGEE224 pKa = 3.59RR225 pKa = 11.84CLEE228 pKa = 4.07GSVVQKK234 pKa = 10.44EE235 pKa = 4.25LQRR238 pKa = 4.96

Molecular weight: 27.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2979

191

1054

496.5

55.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.486 ± 0.473

1.578 ± 0.367

4.23 ± 0.571

6.244 ± 0.657

3.693 ± 0.484

6.949 ± 0.418

2.216 ± 0.387

4.33 ± 0.37

4.901 ± 0.425

8.191 ± 0.902

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.048 ± 0.286

4.532 ± 0.373

5.74 ± 0.566

4.498 ± 0.253

6.949 ± 1.058

10.305 ± 0.799

5.505 ± 0.507

5.27 ± 0.367

1.981 ± 0.244

3.357 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski