Sphingobacteriales bacterium UPWRP_1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; unclassified Sphingobacteriales

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7THF5|A0A2P7THF5_9SPHI Peptidase_M1 domain-containing protein OS=Sphingobacteriales bacterium UPWRP_1 OX=2116516 GN=C7N43_25535 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 9.36KK3 pKa = 9.06TIFLSTFLLITALYY17 pKa = 9.82DD18 pKa = 4.16LKK20 pKa = 11.02AQNWTQIGVDD30 pKa = 3.1IDD32 pKa = 5.34GEE34 pKa = 4.6TEE36 pKa = 4.38DD37 pKa = 3.55NWSGYY42 pKa = 8.7SVSLSANGNIVAIGEE57 pKa = 4.33PLTDD61 pKa = 3.28EE62 pKa = 4.36TGIDD66 pKa = 3.65DD67 pKa = 4.12GQVRR71 pKa = 11.84VYY73 pKa = 10.92QNNDD77 pKa = 3.34GNWTQIGSDD86 pKa = 3.19IVGEE90 pKa = 4.04AAGDD94 pKa = 3.69RR95 pKa = 11.84FGSAVSLSAGGDD107 pKa = 3.19IVAVSAPRR115 pKa = 11.84NDD117 pKa = 4.02GNGTDD122 pKa = 3.49AGHH125 pKa = 5.62VRR127 pKa = 11.84VYY129 pKa = 11.16QNVSGNWTQIGQDD142 pKa = 3.09IDD144 pKa = 3.6GQAADD149 pKa = 4.88DD150 pKa = 3.98RR151 pKa = 11.84SGDD154 pKa = 3.87AVSLSANGSILAIGSVRR171 pKa = 11.84NEE173 pKa = 3.2AWAGDD178 pKa = 3.3VRR180 pKa = 11.84VYY182 pKa = 11.24QNVSGNWTQIGSDD195 pKa = 2.82IVGEE199 pKa = 4.18NPSDD203 pKa = 3.46QSGYY207 pKa = 10.46SVSLNATGNILAIGAFANSDD227 pKa = 3.52NGNLAGGQVRR237 pKa = 11.84VYY239 pKa = 11.1QNVSGNWTQVGQDD252 pKa = 2.91INGYY256 pKa = 7.59FQEE259 pKa = 4.19NLLGYY264 pKa = 9.72SVSLNATGNILAIGAPGVNAAGFAQVFQNISGTWTQIGEE303 pKa = 4.45DD304 pKa = 3.38IYY306 pKa = 11.65GEE308 pKa = 4.03NDD310 pKa = 3.06FDD312 pKa = 4.56EE313 pKa = 5.23SGCSVSLNANGNIVAIGSRR332 pKa = 11.84GVEE335 pKa = 4.12GIGNIDD341 pKa = 3.31GSVRR345 pKa = 11.84VYY347 pKa = 11.26EE348 pKa = 4.21NVSGSWLQTGNTIAGEE364 pKa = 4.08PLNQFPGIAVSLNAGGNILAIGAPYY389 pKa = 10.8NNGNGEE395 pKa = 4.16EE396 pKa = 4.1AGHH399 pKa = 5.3VRR401 pKa = 11.84VYY403 pKa = 7.84QQCDD407 pKa = 3.04INTPPVPTIATLPDD421 pKa = 3.2VTAEE425 pKa = 4.16CSVTTLTPPTATDD438 pKa = 3.45GCGNTVFGTPSVTLPLTSQGTTTVIWIYY466 pKa = 11.2NSGNASSVQTQNVVIDD482 pKa = 4.44DD483 pKa = 3.93VTNPTITCVGNQTVDD498 pKa = 2.91ADD500 pKa = 3.51QSHH503 pKa = 7.38FYY505 pKa = 9.08TVNGTEE511 pKa = 4.39FDD513 pKa = 3.75PTLTSDD519 pKa = 3.14NCGIASVINLYY530 pKa = 8.87TVAFSLAGAQIPEE543 pKa = 4.34GNTTISWTITDD554 pKa = 3.58NAGNNQTCSFVVTVNTYY571 pKa = 10.71VGIEE575 pKa = 3.96TLQQKK580 pKa = 10.28GISIYY585 pKa = 10.2PNPANDD591 pKa = 3.45ILHH594 pKa = 7.2IDD596 pKa = 4.36FAQNNIQKK604 pKa = 10.44LAIKK608 pKa = 9.43DD609 pKa = 3.52IKK611 pKa = 10.68GSSIFEE617 pKa = 4.06KK618 pKa = 10.38TNPNQNEE625 pKa = 4.19TLDD628 pKa = 4.05LSDD631 pKa = 4.72FASGMYY637 pKa = 10.05IMSIQTDD644 pKa = 3.84KK645 pKa = 11.26EE646 pKa = 3.81ILITKK651 pKa = 9.63IVKK654 pKa = 9.03QQ655 pKa = 3.63

Molecular weight: 68.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P7TI51|A0A2P7TI51_9SPHI PKD domain-containing protein (Fragment) OS=Sphingobacteriales bacterium UPWRP_1 OX=2116516 GN=C7N43_24245 PE=4 SV=1
MM1 pKa = 7.4AHH3 pKa = 7.28PKK5 pKa = 9.68RR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 5.03SKK10 pKa = 6.87TRR12 pKa = 11.84RR13 pKa = 11.84DD14 pKa = 3.28KK15 pKa = 11.26RR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 5.36YY20 pKa = 8.65TASVPNVVNCSNCGAPVLSHH40 pKa = 6.49RR41 pKa = 11.84VCGEE45 pKa = 3.63CGFYY49 pKa = 10.38RR50 pKa = 11.84GQLAIAKK57 pKa = 6.44TTAA60 pKa = 2.97

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7781

0

7781

2977425

25

4588

382.7

42.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.435 ± 0.026

1.317 ± 0.018

4.668 ± 0.02

4.973 ± 0.036

4.739 ± 0.022

7.102 ± 0.035

1.847 ± 0.016

6.219 ± 0.023

5.003 ± 0.045

9.625 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.015

6.168 ± 0.031

4.777 ± 0.023

4.565 ± 0.023

3.599 ± 0.026

5.764 ± 0.032

7.209 ± 0.061

6.495 ± 0.023

1.239 ± 0.01

4.196 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski