Bacillus phage Anath

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JUR5|A0A2P1JUR5_9CAUD Uncharacterized protein OS=Bacillus phage Anath OX=2108114 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.55KK3 pKa = 10.31LLLVGALALGVVGLVGCEE21 pKa = 3.55EE22 pKa = 3.58NSYY25 pKa = 10.81YY26 pKa = 10.65FEE28 pKa = 5.07GKK30 pKa = 8.88LQKK33 pKa = 10.11EE34 pKa = 4.25SRR36 pKa = 11.84IQEE39 pKa = 4.18IIEE42 pKa = 4.49DD43 pKa = 3.9RR44 pKa = 11.84LEE46 pKa = 4.27SQLGEE51 pKa = 4.22EE52 pKa = 4.65NDD54 pKa = 3.58MEE56 pKa = 5.86IDD58 pKa = 3.21VDD60 pKa = 4.21VNIMEE65 pKa = 4.4EE66 pKa = 4.24AGDD69 pKa = 3.75EE70 pKa = 4.16

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JUP5|A0A2P1JUP5_9CAUD Uncharacterized protein OS=Bacillus phage Anath OX=2108114 PE=4 SV=1
MM1 pKa = 7.74TYY3 pKa = 10.7FLINAIVIGLIGATAFATWKK23 pKa = 10.37LLLKK27 pKa = 10.71FGFRR31 pKa = 11.84CQRR34 pKa = 11.84DD35 pKa = 3.44KK36 pKa = 11.57KK37 pKa = 10.67FADD40 pKa = 3.26KK41 pKa = 10.28MRR43 pKa = 11.84LVLIFVLLACVVRR56 pKa = 11.84SLMM59 pKa = 4.54

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15790

29

1062

207.8

23.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.036 ± 0.356

0.918 ± 0.121

5.909 ± 0.223

7.612 ± 0.413

4.414 ± 0.167

6.732 ± 0.223

1.963 ± 0.169

6.137 ± 0.16

7.821 ± 0.39

8.075 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.053 ± 0.173

5.054 ± 0.288

2.869 ± 0.204

3.901 ± 0.142

4.56 ± 0.249

5.51 ± 0.385

5.997 ± 0.328

7.099 ± 0.275

1.393 ± 0.148

3.946 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski