Actinomadura sp. RB29

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5700 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P4UD21|A0A2P4UD21_9ACTN Ferrous-iron efflux pump FieF OS=Actinomadura sp. RB29 OX=1926885 GN=fieF_2 PE=4 SV=1
MM1 pKa = 7.37SPSTILRR8 pKa = 11.84QAAADD13 pKa = 3.52AEE15 pKa = 4.47FRR17 pKa = 11.84AEE19 pKa = 4.33LLDD22 pKa = 3.79NPEE25 pKa = 4.14VFGVAAGTVPEE36 pKa = 4.27SVEE39 pKa = 4.17RR40 pKa = 11.84QDD42 pKa = 4.36EE43 pKa = 4.08ASLNYY48 pKa = 7.48WTEE51 pKa = 3.59GVAAVDD57 pKa = 5.03AYY59 pKa = 10.66ACSSTCTSGPFTFACDD75 pKa = 3.54GTTKK79 pKa = 10.8GG80 pKa = 3.96

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P4UEC8|A0A2P4UEC8_9ACTN Alpha/beta hydrolase family protein OS=Actinomadura sp. RB29 OX=1926885 GN=BTM25_45700 PE=4 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84RR3 pKa = 11.84ARR5 pKa = 11.84HH6 pKa = 5.58LRR8 pKa = 11.84RR9 pKa = 11.84ITDD12 pKa = 3.71TARR15 pKa = 11.84TACPRR20 pKa = 11.84RR21 pKa = 11.84AARR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84AVRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84TRR35 pKa = 11.84GPRR38 pKa = 11.84SIPGLRR44 pKa = 11.84GTPSRR49 pKa = 11.84RR50 pKa = 11.84SKK52 pKa = 10.44LRR54 pKa = 11.84PRR56 pKa = 11.84RR57 pKa = 11.84VPRR60 pKa = 11.84LLRR63 pKa = 11.84TPRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84AAGRR72 pKa = 11.84LRR74 pKa = 11.84RR75 pKa = 11.84AVGVCPRR82 pKa = 11.84ARR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84PLVLCLTLSLSPRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84QAPRR105 pKa = 11.84PRR107 pKa = 11.84RR108 pKa = 11.84VRR110 pKa = 11.84RR111 pKa = 11.84LPRR114 pKa = 11.84TRR116 pKa = 11.84LPRR119 pKa = 11.84TRR121 pKa = 11.84RR122 pKa = 11.84TGAVGRR128 pKa = 11.84PRR130 pKa = 11.84RR131 pKa = 11.84VARR134 pKa = 11.84ARR136 pKa = 11.84RR137 pKa = 11.84VLRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84LLVVRR148 pKa = 11.84LTLVLRR154 pKa = 11.84ARR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84PSGAARR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84PSPRR171 pKa = 11.84TRR173 pKa = 11.84PARR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84MPHH181 pKa = 6.07PAGLRR186 pKa = 11.84ALRR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84TTRR195 pKa = 11.84TCRR198 pKa = 11.84RR199 pKa = 11.84AGLCRR204 pKa = 11.84TRR206 pKa = 11.84RR207 pKa = 11.84VRR209 pKa = 11.84RR210 pKa = 11.84TVSRR214 pKa = 11.84RR215 pKa = 11.84LRR217 pKa = 11.84MRR219 pKa = 11.84AGARR223 pKa = 11.84GTGSIAGRR231 pKa = 11.84NGGGLAAGGSAPGRR245 pKa = 11.84RR246 pKa = 11.84CGARR250 pKa = 11.84PRR252 pKa = 11.84LRR254 pKa = 11.84PRR256 pKa = 11.84PRR258 pKa = 11.84RR259 pKa = 11.84RR260 pKa = 11.84VGLRR264 pKa = 11.84PRR266 pKa = 11.84LRR268 pKa = 11.84LRR270 pKa = 11.84VDD272 pKa = 3.14LRR274 pKa = 11.84LRR276 pKa = 11.84LGACPP281 pKa = 3.84

Molecular weight: 32.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5700

0

5700

1924993

29

7640

337.7

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.652 ± 0.055

0.73 ± 0.01

6.322 ± 0.027

5.227 ± 0.033

2.791 ± 0.017

9.489 ± 0.036

2.161 ± 0.016

3.094 ± 0.021

1.829 ± 0.025

10.517 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.608 ± 0.015

1.637 ± 0.016

6.47 ± 0.036

2.293 ± 0.018

8.819 ± 0.044

4.448 ± 0.024

5.79 ± 0.03

8.772 ± 0.033

1.424 ± 0.013

1.927 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski