Microbacterium phage Eden

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Edenvirus; Microbacterium virus Eden

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KWD0|A0A345KWD0_9CAUD Uncharacterized protein OS=Microbacterium phage Eden OX=2250289 GN=37 PE=4 SV=1
MM1 pKa = 7.4YY2 pKa = 10.33SRR4 pKa = 11.84AAAGYY9 pKa = 8.13NRR11 pKa = 11.84SMNTIKK17 pKa = 10.67HH18 pKa = 6.13ADD20 pKa = 3.13ILDD23 pKa = 4.95FINSVEE29 pKa = 4.28HH30 pKa = 6.0VAHH33 pKa = 6.54NCTTEE38 pKa = 3.82EE39 pKa = 4.09FEE41 pKa = 4.68EE42 pKa = 4.69LDD44 pKa = 3.85STSLILDD51 pKa = 4.22VISVVMDD58 pKa = 4.01NADD61 pKa = 3.01EE62 pKa = 4.37WVSHH66 pKa = 6.86LEE68 pKa = 4.43LAITKK73 pKa = 10.0NGYY76 pKa = 6.69EE77 pKa = 4.14LRR79 pKa = 11.84YY80 pKa = 9.65EE81 pKa = 4.1PAEE84 pKa = 4.19SVIGMDD90 pKa = 3.36MDD92 pKa = 5.92HH93 pKa = 7.05IVLDD97 pKa = 4.58LINRR101 pKa = 11.84GLEE104 pKa = 3.98VLTEE108 pKa = 4.12DD109 pKa = 4.9LKK111 pKa = 11.54EE112 pKa = 4.17DD113 pKa = 3.4

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KWC1|A0A345KWC1_9CAUD Deoxyuridine triphosphatase OS=Microbacterium phage Eden OX=2250289 GN=28 PE=4 SV=1
MM1 pKa = 7.24NKK3 pKa = 9.56HH4 pKa = 5.28MKK6 pKa = 10.41ALIKK10 pKa = 10.41KK11 pKa = 8.33VEE13 pKa = 4.12KK14 pKa = 10.42QGGEE18 pKa = 3.73VRR20 pKa = 11.84ITRR23 pKa = 11.84RR24 pKa = 11.84GHH26 pKa = 4.33VQFKK30 pKa = 10.26KK31 pKa = 10.29DD32 pKa = 3.47GRR34 pKa = 11.84VVAVGAGTPSDD45 pKa = 3.56PRR47 pKa = 11.84SWANLSAQLRR57 pKa = 11.84RR58 pKa = 11.84AGFDD62 pKa = 3.1VV63 pKa = 3.49

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12679

35

820

183.8

20.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.255 ± 0.33

0.481 ± 0.092

6.436 ± 0.245

6.31 ± 0.292

3.415 ± 0.146

8.187 ± 0.346

1.893 ± 0.153

4.645 ± 0.286

4.48 ± 0.274

8.628 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.12

3.573 ± 0.253

4.803 ± 0.246

3.541 ± 0.219

6.089 ± 0.307

5.592 ± 0.304

6.704 ± 0.366

7.161 ± 0.316

2.193 ± 0.151

2.366 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski