Lactobacillus phage phiAQ113

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0P7E1|L0P7E1_9CAUD Putative transcriptional regulator OS=Lactobacillus phage phiAQ113 OX=1206110 GN=BN107_000054 PE=4 SV=1
MM1 pKa = 8.22IEE3 pKa = 5.18FDD5 pKa = 5.56DD6 pKa = 4.98KK7 pKa = 11.46DD8 pKa = 3.91LRR10 pKa = 11.84DD11 pKa = 3.75LQEE14 pKa = 3.48VDD16 pKa = 5.04GIVLQDD22 pKa = 3.07VHH24 pKa = 6.34GKK26 pKa = 9.51RR27 pKa = 11.84VAIGKK32 pKa = 9.78GFDD35 pKa = 3.5YY36 pKa = 11.52GNVFQFMTDD45 pKa = 3.14YY46 pKa = 10.55FDD48 pKa = 4.35EE49 pKa = 4.7YY50 pKa = 10.95GAEE53 pKa = 4.12DD54 pKa = 3.72FAKK57 pKa = 10.0QVGYY61 pKa = 10.76EE62 pKa = 4.12DD63 pKa = 3.55VVEE66 pKa = 4.63MFKK69 pKa = 10.75EE70 pKa = 4.15WFSGILFNEE79 pKa = 4.35SDD81 pKa = 3.68LMDD84 pKa = 3.9WVCEE88 pKa = 4.12SFAGIDD94 pKa = 3.57ADD96 pKa = 4.64SLQDD100 pKa = 3.26QYY102 pKa = 12.1DD103 pKa = 4.04YY104 pKa = 11.79EE105 pKa = 5.2NEE107 pKa = 4.38DD108 pKa = 3.37YY109 pKa = 11.51LEE111 pKa = 5.78AEE113 pKa = 4.27DD114 pKa = 5.45HH115 pKa = 7.23KK116 pKa = 11.31LDD118 pKa = 4.13QEE120 pKa = 4.21RR121 pKa = 11.84GKK123 pKa = 10.85

Molecular weight: 14.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0P6F6|L0P6F6_9CAUD ERF Recombinase OS=Lactobacillus phage phiAQ113 OX=1206110 GN=BN107_000051 PE=4 SV=1
MM1 pKa = 7.46NNNTEE6 pKa = 3.77VWKK9 pKa = 10.54DD10 pKa = 3.42VQGYY14 pKa = 8.7EE15 pKa = 4.54GLYY18 pKa = 9.74QVSNLGRR25 pKa = 11.84VRR27 pKa = 11.84SLDD30 pKa = 3.19RR31 pKa = 11.84TIIDD35 pKa = 3.49SIGRR39 pKa = 11.84KK40 pKa = 8.94RR41 pKa = 11.84LYY43 pKa = 10.01KK44 pKa = 10.69GKK46 pKa = 9.91LLKK49 pKa = 10.22PINDD53 pKa = 3.03SHH55 pKa = 7.99GYY57 pKa = 9.91FEE59 pKa = 5.08VRR61 pKa = 11.84LCKK64 pKa = 9.5GTNRR68 pKa = 11.84KK69 pKa = 8.43PPHH72 pKa = 5.19IHH74 pKa = 6.77RR75 pKa = 11.84LVAQAFLPNPKK86 pKa = 9.8KK87 pKa = 10.66LPCINHH93 pKa = 6.13IDD95 pKa = 3.76EE96 pKa = 4.78NKK98 pKa = 10.08QNNAVSNLEE107 pKa = 3.93WCSYY111 pKa = 10.32KK112 pKa = 10.95YY113 pKa = 10.9NNNYY117 pKa = 7.28GTRR120 pKa = 11.84TDD122 pKa = 3.45RR123 pKa = 11.84AIQTRR128 pKa = 11.84SRR130 pKa = 11.84NPEE133 pKa = 3.43WYY135 pKa = 9.99KK136 pKa = 10.64KK137 pKa = 10.05IKK139 pKa = 9.8EE140 pKa = 4.14HH141 pKa = 5.91TGKK144 pKa = 10.12IGKK147 pKa = 9.77KK148 pKa = 8.38YY149 pKa = 10.88GRR151 pKa = 11.84INGAKK156 pKa = 8.49MSKK159 pKa = 10.0PVLQFDD165 pKa = 4.53LDD167 pKa = 3.66GDD169 pKa = 5.03FIRR172 pKa = 11.84EE173 pKa = 4.12WPSMRR178 pKa = 11.84EE179 pKa = 3.71VKK181 pKa = 10.55RR182 pKa = 11.84NLKK185 pKa = 9.67IDD187 pKa = 3.42NSSIARR193 pKa = 11.84CCKK196 pKa = 10.1GKK198 pKa = 10.42QKK200 pKa = 9.44TAGKK204 pKa = 9.62YY205 pKa = 7.3IWKK208 pKa = 10.05FKK210 pKa = 10.89

Molecular weight: 24.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10802

47

748

192.9

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.175 ± 0.572

0.676 ± 0.125

6.693 ± 0.413

5.999 ± 0.477

3.536 ± 0.205

5.795 ± 0.413

1.574 ± 0.168

6.304 ± 0.227

9.322 ± 0.419

7.897 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.197

6.693 ± 0.276

3.259 ± 0.191

4.268 ± 0.277

3.722 ± 0.287

6.425 ± 0.366

5.999 ± 0.406

6.619 ± 0.319

1.157 ± 0.139

4.221 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski