Hylemonella gracilis ATCC 19624

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hylemonella; Hylemonella gracilis

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3342 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3KU97|F3KU97_9BURK Major facilitator superfamily MFS_1 OS=Hylemonella gracilis ATCC 19624 OX=887062 GN=HGR_10145 PE=4 SV=1
MM1 pKa = 7.18SAVAEE6 pKa = 4.44SVQTDD11 pKa = 3.51AVSEE15 pKa = 4.33MPAPILFTDD24 pKa = 3.95SAAAKK29 pKa = 10.02VADD32 pKa = 5.45LIAEE36 pKa = 4.31EE37 pKa = 4.82GNPDD41 pKa = 2.99LKK43 pKa = 11.13LRR45 pKa = 11.84VFVQGGGCSGFQYY58 pKa = 10.9GFTFDD63 pKa = 3.69EE64 pKa = 4.74VANEE68 pKa = 4.9DD69 pKa = 4.01DD70 pKa = 3.44TTMTKK75 pKa = 10.66NGVSLLIDD83 pKa = 3.54AMSYY87 pKa = 10.32QYY89 pKa = 11.5LVGAEE94 pKa = 3.79IDD96 pKa = 3.91YY97 pKa = 11.36KK98 pKa = 11.19EE99 pKa = 4.67DD100 pKa = 3.23LQGAQFVIKK109 pKa = 10.4NPNATTTCGCGSSFSAA125 pKa = 4.79

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3KR16|F3KR16_9BURK Methionine import ATP-binding protein MetN OS=Hylemonella gracilis ATCC 19624 OX=887062 GN=metN PE=3 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.67MKK5 pKa = 9.88TKK7 pKa = 10.49SAAKK11 pKa = 9.4KK12 pKa = 9.52RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.62RR24 pKa = 11.84GQAFKK29 pKa = 11.01RR30 pKa = 11.84HH31 pKa = 5.79ILTKK35 pKa = 9.78KK36 pKa = 3.94TTKK39 pKa = 10.22NKK41 pKa = 8.75RR42 pKa = 11.84HH43 pKa = 5.87LRR45 pKa = 11.84GAVNVHH51 pKa = 5.57ATNMGHH57 pKa = 6.24MAQMLPGQGLL67 pKa = 3.4

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3342

0

3342

1084002

20

1969

324.4

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.766 ± 0.062

0.818 ± 0.012

5.075 ± 0.03

5.358 ± 0.041

3.332 ± 0.025

8.279 ± 0.039

2.277 ± 0.021

4.233 ± 0.03

3.288 ± 0.036

11.302 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.019

2.541 ± 0.021

5.227 ± 0.034

4.362 ± 0.029

6.924 ± 0.045

5.388 ± 0.028

5.152 ± 0.027

7.554 ± 0.033

1.49 ± 0.021

2.242 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski