Rhizobium gallicum bv. gallicum R602

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; Rhizobium gallicum; Rhizobium gallicum bv. gallicum

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4X786|A0A0B4X786_9RHIZ CbtA superfamily transporter protein OS=Rhizobium gallicum bv. gallicum R602 OX=1418105 GN=RGR602_CH03280 PE=4 SV=1
MM1 pKa = 7.38ATLTVNADD9 pKa = 3.18YY10 pKa = 11.16GLDD13 pKa = 3.28MRR15 pKa = 11.84GIDD18 pKa = 5.13FGWLPYY24 pKa = 10.39ADD26 pKa = 4.12SYY28 pKa = 11.56TYY30 pKa = 11.18GSTIFAAHH38 pKa = 6.63FSDD41 pKa = 3.68GTVEE45 pKa = 4.02EE46 pKa = 4.3FRR48 pKa = 11.84GRR50 pKa = 11.84GFSYY54 pKa = 10.87DD55 pKa = 3.28SDD57 pKa = 3.98GVPTGGTVRR66 pKa = 11.84SYY68 pKa = 12.04ALFDD72 pKa = 3.38NGNRR76 pKa = 11.84VSYY79 pKa = 11.23VDD81 pKa = 3.69GVSIAVTSIVDD92 pKa = 3.29AAEE95 pKa = 4.41TYY97 pKa = 7.57TTSDD101 pKa = 4.12DD102 pKa = 3.28IAVIGRR108 pKa = 11.84ALAGNDD114 pKa = 3.24VLRR117 pKa = 11.84GGGIFDD123 pKa = 4.06YY124 pKa = 11.24LRR126 pKa = 11.84GFAGNDD132 pKa = 3.3TLYY135 pKa = 11.42GNAGNDD141 pKa = 3.62TLVGDD146 pKa = 5.22DD147 pKa = 5.25GNDD150 pKa = 3.91TINGGAGKK158 pKa = 10.12DD159 pKa = 3.59RR160 pKa = 11.84IDD162 pKa = 3.92GGVGSDD168 pKa = 3.1TASYY172 pKa = 10.68AGASSGVVASLGNPGGNTQNAYY194 pKa = 9.97GDD196 pKa = 3.64IYY198 pKa = 10.87ISIEE202 pKa = 3.99SLAGSSHH209 pKa = 7.4ADD211 pKa = 3.14RR212 pKa = 11.84LYY214 pKa = 11.47GNAATNYY221 pKa = 10.14LSGDD225 pKa = 3.3AGNDD229 pKa = 3.37YY230 pKa = 11.15LSASGGNDD238 pKa = 2.88WLHH241 pKa = 6.5GGSGADD247 pKa = 3.3QLVGGSGADD256 pKa = 3.21RR257 pKa = 11.84FIFKK261 pKa = 10.37AWGDD265 pKa = 3.64HH266 pKa = 6.29SGDD269 pKa = 4.06SILDD273 pKa = 3.56FTATQSDD280 pKa = 4.49RR281 pKa = 11.84IDD283 pKa = 3.45MSKK286 pKa = 10.07IDD288 pKa = 4.41ANQSIAGDD296 pKa = 3.52QAFSFIGTAAFSGKK310 pKa = 10.12AGEE313 pKa = 4.09LRR315 pKa = 11.84FVKK318 pKa = 9.53TASDD322 pKa = 3.56TYY324 pKa = 10.87IYY326 pKa = 11.1GDD328 pKa = 3.63VNGDD332 pKa = 2.99ASTDD336 pKa = 3.72YY337 pKa = 10.73TIHH340 pKa = 7.42LDD342 pKa = 3.93DD343 pKa = 6.12AVTIWNSYY351 pKa = 10.17FYY353 pKa = 11.29LL354 pKa = 5.37

Molecular weight: 36.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4WWL8|A0A0B4WWL8_9RHIZ Phosphoenolpyruvate carboxykinase (ATP) OS=Rhizobium gallicum bv. gallicum R602 OX=1418105 GN=pckA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.1GGRR28 pKa = 11.84KK29 pKa = 9.65VIVARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7034

0

7034

2066382

29

3168

293.8

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.746 ± 0.037

0.881 ± 0.01

5.575 ± 0.022

5.789 ± 0.023

3.97 ± 0.02

8.191 ± 0.025

2.053 ± 0.015

5.779 ± 0.02

3.793 ± 0.026

9.921 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.014

2.891 ± 0.015

4.886 ± 0.023

3.121 ± 0.019

6.752 ± 0.028

5.922 ± 0.02

5.233 ± 0.02

7.306 ± 0.025

1.283 ± 0.012

2.336 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski