Elusimicrobium sp. An273

Taxonomy: cellular organisms; Bacteria; Elusimicrobia; Elusimicrobia; Elusimicrobiales; Elusimicrobiaceae; Elusimicrobium; unclassified Elusimicrobium

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1395 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4DFR0|A0A1Y4DFR0_9BACT Uncharacterized protein OS=Elusimicrobium sp. An273 OX=1965617 GN=B5F75_04820 PE=4 SV=1
MM1 pKa = 7.18VKK3 pKa = 9.97YY4 pKa = 10.13VCEE7 pKa = 4.3VCGYY11 pKa = 9.03TYY13 pKa = 10.98DD14 pKa = 4.11PAVGDD19 pKa = 4.17PDD21 pKa = 3.73NGVPAGTKK29 pKa = 9.33WEE31 pKa = 4.16DD32 pKa = 3.39VKK34 pKa = 11.27EE35 pKa = 4.13DD36 pKa = 3.72WVCPVCGVGKK46 pKa = 10.01DD47 pKa = 3.4QFKK50 pKa = 10.96PEE52 pKa = 3.84NN53 pKa = 3.58

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4DES5|A0A1Y4DES5_9BACT Uncharacterized protein OS=Elusimicrobium sp. An273 OX=1965617 GN=B5F75_02545 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 9.08KK3 pKa = 8.18TLKK6 pKa = 9.71TLWRR10 pKa = 11.84LLLHH14 pKa = 6.44FFFPRR19 pKa = 11.84TCFACGCDD27 pKa = 3.73LPWRR31 pKa = 11.84RR32 pKa = 11.84EE33 pKa = 3.94EE34 pKa = 4.21PLCAACAQGLKK45 pKa = 9.58TPGPHH50 pKa = 5.34ICRR53 pKa = 11.84RR54 pKa = 11.84CGAVLTEE61 pKa = 5.09GGAHH65 pKa = 7.15CYY67 pKa = 10.03ACRR70 pKa = 11.84GSKK73 pKa = 9.3EE74 pKa = 3.57RR75 pKa = 11.84AYY77 pKa = 9.31KK78 pKa = 10.5CRR80 pKa = 11.84VIRR83 pKa = 11.84SAFVFNTSSRR93 pKa = 11.84ALVHH97 pKa = 5.74ALKK100 pKa = 10.9YY101 pKa = 10.77AGADD105 pKa = 3.24YY106 pKa = 10.46LARR109 pKa = 11.84YY110 pKa = 8.1MGGLMAKK117 pKa = 10.0RR118 pKa = 11.84FARR121 pKa = 11.84YY122 pKa = 8.87PEE124 pKa = 3.93LAAAEE129 pKa = 4.29VVMPVPLFPRR139 pKa = 11.84RR140 pKa = 11.84GRR142 pKa = 11.84KK143 pKa = 8.94RR144 pKa = 11.84GYY146 pKa = 9.28NQSEE150 pKa = 3.76LLARR154 pKa = 11.84FFAPQVGLPVQTKK167 pKa = 9.61ALVRR171 pKa = 11.84VRR173 pKa = 11.84NTVSQTKK180 pKa = 10.13LGRR183 pKa = 11.84AGRR186 pKa = 11.84LQNMAGAFACRR197 pKa = 11.84RR198 pKa = 11.84PEE200 pKa = 3.83WVKK203 pKa = 11.0GKK205 pKa = 8.03TVLLIDD211 pKa = 4.8DD212 pKa = 4.2VATTGATLEE221 pKa = 4.37GCAAALKK228 pKa = 10.27AAGARR233 pKa = 11.84RR234 pKa = 11.84VLAYY238 pKa = 9.2TFARR242 pKa = 11.84EE243 pKa = 3.86

Molecular weight: 26.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1395

0

1395

459124

26

3027

329.1

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.044 ± 0.087

1.251 ± 0.029

4.839 ± 0.042

6.102 ± 0.055

4.544 ± 0.051

6.913 ± 0.061

1.769 ± 0.029

5.57 ± 0.059

6.804 ± 0.071

10.075 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.029

3.889 ± 0.041

4.544 ± 0.05

4.108 ± 0.055

4.839 ± 0.049

5.526 ± 0.048

5.39 ± 0.049

6.895 ± 0.053

1.093 ± 0.027

3.39 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski