Lake Sarah-associated circular virus-41

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA81|A0A126GA81_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-41 OX=1685770 PE=4 SV=1
MM1 pKa = 7.69ANGKK5 pKa = 7.14STRR8 pKa = 11.84RR9 pKa = 11.84QGIFWLCTIPSPNEE23 pKa = 3.27VCAQLEE29 pKa = 4.56LGTLPVGLVWCKK41 pKa = 9.04GQKK44 pKa = 9.33EE45 pKa = 4.23RR46 pKa = 11.84GSGTGYY52 pKa = 10.07EE53 pKa = 4.47HH54 pKa = 6.47YY55 pKa = 10.8QLVAAFAKK63 pKa = 10.08KK64 pKa = 10.04VSLPGVVGMFGRR76 pKa = 11.84GTHH79 pKa = 6.3GEE81 pKa = 4.04LSRR84 pKa = 11.84SEE86 pKa = 4.07AANEE90 pKa = 3.88YY91 pKa = 9.79VGKK94 pKa = 10.34EE95 pKa = 3.75EE96 pKa = 4.11TRR98 pKa = 11.84IGTTFEE104 pKa = 4.97LGAKK108 pKa = 9.57PIRR111 pKa = 11.84RR112 pKa = 11.84NSKK115 pKa = 8.97TDD117 pKa = 3.12WEE119 pKa = 4.97SVWTAAKK126 pKa = 10.54SGDD129 pKa = 3.9LSAVPANVRR138 pKa = 11.84VVNYY142 pKa = 9.29HH143 pKa = 6.17ALRR146 pKa = 11.84AIRR149 pKa = 11.84SDD151 pKa = 3.1HH152 pKa = 5.96AVPVGVEE159 pKa = 3.97RR160 pKa = 11.84TVHH163 pKa = 5.15VFWGSTGTGKK173 pKa = 10.02SRR175 pKa = 11.84RR176 pKa = 11.84AWEE179 pKa = 3.79EE180 pKa = 3.55AGIDD184 pKa = 4.16AYY186 pKa = 8.93TKK188 pKa = 10.48CPRR191 pKa = 11.84SKK193 pKa = 10.48FWDD196 pKa = 4.98GYY198 pKa = 10.19QDD200 pKa = 3.52QQHH203 pKa = 5.16VVVDD207 pKa = 4.2EE208 pKa = 4.15FRR210 pKa = 11.84GGIDD214 pKa = 3.25VAHH217 pKa = 7.59LLRR220 pKa = 11.84WFDD223 pKa = 3.83RR224 pKa = 11.84YY225 pKa = 9.38PVRR228 pKa = 11.84VEE230 pKa = 3.72IKK232 pKa = 9.65GSSRR236 pKa = 11.84PLVANTIWITSNKK249 pKa = 10.05DD250 pKa = 3.12PVEE253 pKa = 4.22WYY255 pKa = 9.79PEE257 pKa = 3.88LDD259 pKa = 3.67LATLEE264 pKa = 4.13ALLRR268 pKa = 11.84RR269 pKa = 11.84LTIVHH274 pKa = 6.7FPP276 pKa = 3.71

Molecular weight: 30.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA81|A0A126GA81_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-41 OX=1685770 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.42RR3 pKa = 11.84KK4 pKa = 9.22GHH6 pKa = 6.11YY7 pKa = 7.25PTPDD11 pKa = 2.88SSRR14 pKa = 11.84SRR16 pKa = 11.84DD17 pKa = 3.17RR18 pKa = 11.84KK19 pKa = 8.81RR20 pKa = 11.84AKK22 pKa = 9.93HH23 pKa = 5.61SRR25 pKa = 11.84PTIPSRR31 pKa = 11.84PLTHH35 pKa = 6.83RR36 pKa = 11.84VVQAHH41 pKa = 5.52TNYY44 pKa = 9.93INAIASRR51 pKa = 11.84YY52 pKa = 7.94NRR54 pKa = 11.84AVVGDD59 pKa = 3.8FLGGVPGAIIGAATSNVPFTIKK81 pKa = 9.35KK82 pKa = 8.5TIMGRR87 pKa = 11.84GNPAKK92 pKa = 10.63KK93 pKa = 9.94LVQRR97 pKa = 11.84SAMTSKK103 pKa = 10.77GKK105 pKa = 10.31NKK107 pKa = 10.22LKK109 pKa = 10.0TYY111 pKa = 10.45KK112 pKa = 10.02PYY114 pKa = 10.73KK115 pKa = 10.39LSDD118 pKa = 3.45KK119 pKa = 10.25FVKK122 pKa = 10.21SVKK125 pKa = 9.85QVLQTTCSTGTYY137 pKa = 7.21VTVRR141 pKa = 11.84QGLVGTLMNTAASGSMIGNDD161 pKa = 3.98LGQTGINQIWYY172 pKa = 8.28PGSSNPAGSRR182 pKa = 11.84TLFNALATLSDD193 pKa = 3.65AAATDD198 pKa = 3.88STVVAQTGLNFFTPAKK214 pKa = 9.81IIDD217 pKa = 3.87AASVLFNNKK226 pKa = 9.22GLGDD230 pKa = 4.25PYY232 pKa = 11.36SSAGNLATEE241 pKa = 5.01FTTADD246 pKa = 3.28GSIPTSNTAAGALKK260 pKa = 10.48INVLKK265 pKa = 10.84SYY267 pKa = 11.59VDD269 pKa = 4.48FEE271 pKa = 4.59MKK273 pKa = 10.49NLSNRR278 pKa = 11.84VAYY281 pKa = 10.36VDD283 pKa = 3.03IWEE286 pKa = 4.86CSPTLKK292 pKa = 10.41FQNINPLQVLQQTAAVWDD310 pKa = 3.87STSTINRR317 pKa = 11.84NITYY321 pKa = 7.99ATANANPNEE330 pKa = 4.12IFLDD334 pKa = 3.82STVDD338 pKa = 3.36GMGVLKK344 pKa = 10.42KK345 pKa = 10.72YY346 pKa = 10.69AGLPMSWKK354 pKa = 9.32KK355 pKa = 10.86RR356 pKa = 11.84SMVLAPDD363 pKa = 3.97EE364 pKa = 4.74TCLHH368 pKa = 6.15TVQGPKK374 pKa = 10.66GILDD378 pKa = 3.39WSKK381 pKa = 10.96LVSTQPSVAPATGQVNINLNCLLKK405 pKa = 10.51GFSVGCVISVRR416 pKa = 11.84TDD418 pKa = 2.99QVFQSGIPMYY428 pKa = 10.3GAKK431 pKa = 8.52KK432 pKa = 8.85TYY434 pKa = 10.71NDD436 pKa = 3.91GANLRR441 pKa = 11.84MAVPVAIEE449 pKa = 3.93MKK451 pKa = 10.0EE452 pKa = 4.15SYY454 pKa = 9.95KK455 pKa = 10.77VAVPEE460 pKa = 3.92VAGFVSRR467 pKa = 11.84AVVGGSAQPLNLRR480 pKa = 11.84KK481 pKa = 9.41PRR483 pKa = 11.84FVLWNQVNANGTTNVNKK500 pKa = 10.51LYY502 pKa = 10.74LSNEE506 pKa = 4.1LNPVYY511 pKa = 9.9DD512 pKa = 3.69TTQAQGG518 pKa = 3.0

Molecular weight: 55.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

794

276

518

397.0

43.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.816 ± 0.511

1.134 ± 0.191

3.904 ± 0.049

3.526 ± 2.03

3.023 ± 0.144

8.312 ± 0.67

1.637 ± 0.763

4.408 ± 0.256

6.297 ± 0.741

7.431 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.628

5.919 ± 1.827

5.164 ± 0.274

3.526 ± 0.599

5.542 ± 1.469

7.431 ± 0.769

7.935 ± 0.854

9.068 ± 0.432

2.015 ± 0.972

3.149 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski