Chimpanzee faeces associated microphage 1

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A186YBN9|A0A186YBN9_9VIRU Minor CP 2 OS=Chimpanzee faeces associated microphage 1 OX=1676181 PE=4 SV=1
MM1 pKa = 7.79KK2 pKa = 10.5FFTLFDD8 pKa = 3.73KK9 pKa = 10.74PSPVGTACNTPSLTVQSEE27 pKa = 4.12KK28 pKa = 10.92AQCDD32 pKa = 3.02INAIIARR39 pKa = 11.84YY40 pKa = 9.21KK41 pKa = 8.51KK42 pKa = 9.09TGVVDD47 pKa = 4.59HH48 pKa = 6.74IQRR51 pKa = 11.84GEE53 pKa = 3.88PLYY56 pKa = 10.91ADD58 pKa = 4.42CEE60 pKa = 4.11QAITDD65 pKa = 4.07LAKK68 pKa = 10.83ARR70 pKa = 11.84ILVEE74 pKa = 4.09DD75 pKa = 4.31TEE77 pKa = 4.65DD78 pKa = 4.25AFWTLPSSVRR88 pKa = 11.84DD89 pKa = 3.92VIGEE93 pKa = 3.98PSNLPSWASANRR105 pKa = 11.84SEE107 pKa = 4.05AEE109 pKa = 4.03KK110 pKa = 11.15YY111 pKa = 10.94GFLAPQATPPADD123 pKa = 3.42SVTPPPTDD131 pKa = 3.11SAANGG136 pKa = 3.47

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A186YBP1|A0A186YBP1_9VIRU Major CP OS=Chimpanzee faeces associated microphage 1 OX=1676181 PE=3 SV=1
MM1 pKa = 7.78AFGIDD6 pKa = 4.05DD7 pKa = 4.28ALMAAVAGGTQGAMNLGGAVLGDD30 pKa = 3.49YY31 pKa = 10.52YY32 pKa = 11.37NRR34 pKa = 11.84KK35 pKa = 8.98AASKK39 pKa = 8.81QNKK42 pKa = 8.73VSWEE46 pKa = 3.8MMLANQQWLEE56 pKa = 4.03RR57 pKa = 11.84MSNTAHH63 pKa = 5.48QRR65 pKa = 11.84EE66 pKa = 4.52VADD69 pKa = 4.56LRR71 pKa = 11.84AAGLNPILSATGGSGASSPSYY92 pKa = 10.28AASAPDD98 pKa = 4.02LSALGKK104 pKa = 10.27SGEE107 pKa = 4.23GFSRR111 pKa = 11.84VGNGVMDD118 pKa = 4.01KK119 pKa = 10.9ALRR122 pKa = 11.84AASLRR127 pKa = 11.84SEE129 pKa = 4.43LKK131 pKa = 10.94LMEE134 pKa = 4.85SNANNAEE141 pKa = 3.94AQAANSWEE149 pKa = 3.87NVLYY153 pKa = 10.44TKK155 pKa = 11.16ANTAKK160 pKa = 9.96VLAEE164 pKa = 3.95AGVFQSQLDD173 pKa = 3.88RR174 pKa = 11.84IGWLKK179 pKa = 10.81KK180 pKa = 10.12NSPNTWYY187 pKa = 10.63LWGDD191 pKa = 3.34KK192 pKa = 10.7DD193 pKa = 3.72PSVWQLLPGFSRR205 pKa = 11.84GSEE208 pKa = 3.94FGAQSVGNGVKK219 pKa = 9.88SVYY222 pKa = 10.77DD223 pKa = 3.61FVKK226 pKa = 10.73DD227 pKa = 4.53PIASAVKK234 pKa = 9.95KK235 pKa = 10.48VYY237 pKa = 10.6NAVKK241 pKa = 10.73DD242 pKa = 4.81FGANNASSSSNQSIGYY258 pKa = 9.26SSYY261 pKa = 11.84DD262 pKa = 3.23KK263 pKa = 11.21GSSSDD268 pKa = 3.42SGKK271 pKa = 10.32VPLTTTRR278 pKa = 11.84WRR280 pKa = 11.84KK281 pKa = 8.19GQPLRR286 pKa = 11.84SGKK289 pKa = 9.74FDD291 pKa = 3.5YY292 pKa = 11.27QEE294 pKa = 3.79VRR296 pKa = 11.84RR297 pKa = 11.84KK298 pKa = 10.06RR299 pKa = 11.84LEE301 pKa = 3.92NLKK304 pKa = 10.57KK305 pKa = 10.71

Molecular weight: 32.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1253

136

537

313.3

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.46 ± 1.653

1.676 ± 0.749

6.385 ± 0.493

4.389 ± 0.805

4.549 ± 0.679

7.502 ± 0.836

1.756 ± 0.676

3.033 ± 0.65

4.709 ± 0.987

8.22 ± 0.602

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.472

4.868 ± 0.834

5.108 ± 0.951

3.352 ± 0.537

6.065 ± 0.947

9.417 ± 1.088

5.666 ± 1.19

6.145 ± 0.349

2.314 ± 0.171

3.911 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski