Muribaculaceae bacterium Isolate-114 (HZI)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2793 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2NS17|A0A3N2NS17_9BACT AsmA_2 domain-containing protein OS=Muribaculaceae bacterium Isolate-114 (HZI) OX=2486475 GN=EEL53_01400 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.18KK3 pKa = 10.16YY4 pKa = 10.37RR5 pKa = 11.84CEE7 pKa = 4.01VCDD10 pKa = 3.6WVYY13 pKa = 11.49DD14 pKa = 4.05PAVGDD19 pKa = 3.99PDD21 pKa = 4.71GGIAPGTAFEE31 pKa = 5.98DD32 pKa = 3.81IPEE35 pKa = 4.24DD36 pKa = 3.64WVCPVCGVGKK46 pKa = 10.35DD47 pKa = 3.47EE48 pKa = 5.05FVVEE52 pKa = 4.37EE53 pKa = 4.21

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2NLK8|A0A3N2NLK8_9BACT IS4 family transposase OS=Muribaculaceae bacterium Isolate-114 (HZI) OX=2486475 GN=EEL53_04150 PE=4 SV=1
MM1 pKa = 7.46NKK3 pKa = 9.11GRR5 pKa = 11.84KK6 pKa = 8.5GKK8 pKa = 10.14GVPSLGNIPSGLPVIVTGASGSIGRR33 pKa = 11.84EE34 pKa = 3.31ICRR37 pKa = 11.84ALARR41 pKa = 11.84LGVPVIMACRR51 pKa = 11.84SEE53 pKa = 4.21ARR55 pKa = 11.84YY56 pKa = 8.98AQTYY60 pKa = 10.42GEE62 pKa = 4.62LLRR65 pKa = 11.84EE66 pKa = 4.6FPAFDD71 pKa = 4.47AVFLPLDD78 pKa = 4.09LNMSSSVTEE87 pKa = 3.59AVNRR91 pKa = 11.84LKK93 pKa = 10.69GVRR96 pKa = 11.84LAGIINNAGTMEE108 pKa = 4.13RR109 pKa = 11.84HH110 pKa = 5.99FSLSADD116 pKa = 3.65GPEE119 pKa = 3.7TTMNVNYY126 pKa = 10.54HH127 pKa = 4.87NTRR130 pKa = 11.84LLNQLLLPQIAPGGSVVFTTSLTRR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84WGTRR160 pKa = 11.84HH161 pKa = 6.34LPATVTEE168 pKa = 4.39EE169 pKa = 4.09SFGQLRR175 pKa = 11.84SYY177 pKa = 10.93SLSKK181 pKa = 10.75AWITRR186 pKa = 11.84FAAGFASEE194 pKa = 4.22GKK196 pKa = 9.66KK197 pKa = 10.56CGVRR201 pKa = 11.84VNCADD206 pKa = 3.94PGVVDD211 pKa = 3.75SSMIRR216 pKa = 11.84MDD218 pKa = 3.0RR219 pKa = 11.84WFDD222 pKa = 4.32PIADD226 pKa = 3.21IVFRR230 pKa = 11.84PLIRR234 pKa = 11.84TPRR237 pKa = 11.84NGAVPALRR245 pKa = 11.84ALLSDD250 pKa = 3.52EE251 pKa = 4.02TSRR254 pKa = 11.84IYY256 pKa = 9.64TLRR259 pKa = 11.84ASLPLNKK266 pKa = 10.16

Molecular weight: 28.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2793

0

2793

1002792

38

2602

359.0

40.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.611 ± 0.043

1.232 ± 0.017

6.069 ± 0.034

6.462 ± 0.047

4.41 ± 0.033

7.119 ± 0.04

1.792 ± 0.019

6.8 ± 0.042

6.1 ± 0.039

8.528 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.019

4.998 ± 0.044

3.981 ± 0.027

2.736 ± 0.025

5.287 ± 0.04

6.645 ± 0.039

5.498 ± 0.031

6.544 ± 0.038

1.296 ± 0.021

4.08 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski