Escherichia phage 186 (Bacteriophage 186)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Eganvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O80308|O80308_BP186 Holin OS=Escherichia phage 186 OX=29252 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.07VRR4 pKa = 11.84SMQGDD9 pKa = 3.73TLDD12 pKa = 4.33TICARR17 pKa = 11.84YY18 pKa = 9.13YY19 pKa = 10.72GRR21 pKa = 11.84TEE23 pKa = 4.12GVVEE27 pKa = 4.42TVLQANPGLSEE38 pKa = 4.97LGVILPNGTEE48 pKa = 3.6IDD50 pKa = 4.05LPDD53 pKa = 3.65VASSPVTKK61 pKa = 10.16TINLWEE67 pKa = 4.07

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O80314|O80314_BP186 Baseplate protein OS=Escherichia phage 186 OX=29252 PE=4 SV=1
MM1 pKa = 7.63SEE3 pKa = 3.88LTALQEE9 pKa = 4.15RR10 pKa = 11.84LAGLIASLSPAARR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 8.47MAAEE29 pKa = 3.7IAKK32 pKa = 10.15KK33 pKa = 10.4LRR35 pKa = 11.84TSQQQRR41 pKa = 11.84IKK43 pKa = 10.49RR44 pKa = 11.84QQAPDD49 pKa = 3.64GTPYY53 pKa = 10.8AARR56 pKa = 11.84KK57 pKa = 7.48RR58 pKa = 11.84QPVRR62 pKa = 11.84SKK64 pKa = 10.12KK65 pKa = 10.41GRR67 pKa = 11.84IKK69 pKa = 10.81RR70 pKa = 11.84EE71 pKa = 3.62MFAKK75 pKa = 10.36LRR77 pKa = 11.84TSRR80 pKa = 11.84FMKK83 pKa = 10.72AKK85 pKa = 9.99GSDD88 pKa = 3.12SAAVVEE94 pKa = 4.96FTGKK98 pKa = 6.61VQRR101 pKa = 11.84MARR104 pKa = 11.84VHH106 pKa = 6.18QYY108 pKa = 10.55GLKK111 pKa = 10.37DD112 pKa = 3.34RR113 pKa = 11.84PNRR116 pKa = 11.84NSRR119 pKa = 11.84DD120 pKa = 3.47VQYY123 pKa = 10.64EE124 pKa = 3.73ARR126 pKa = 11.84PLLGFTRR133 pKa = 11.84DD134 pKa = 3.81DD135 pKa = 3.46EE136 pKa = 4.68QMIEE140 pKa = 4.14DD141 pKa = 4.57VIISHH146 pKa = 7.22LGKK149 pKa = 10.52

Molecular weight: 17.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

5

46

9877

58

812

214.7

23.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.912 ± 0.467

1.124 ± 0.191

6.156 ± 0.262

6.449 ± 0.277

3.483 ± 0.265

7.037 ± 0.329

1.863 ± 0.21

5.487 ± 0.234

5.629 ± 0.312

8.697 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.169

3.807 ± 0.262

4.141 ± 0.238

3.766 ± 0.248

6.54 ± 0.405

6.166 ± 0.274

6.267 ± 0.345

6.682 ± 0.348

1.529 ± 0.131

2.622 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski