Ruminococcus albus SY3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; Ruminococcus albus

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011VVA6|A0A011VVA6_RUMAL Diguanylate cyclase OS=Ruminococcus albus SY3 OX=1341156 GN=RASY3_04210 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.13PIIKK6 pKa = 10.03RR7 pKa = 11.84LLSGVLSSTMTISAIPIVSAHH28 pKa = 6.57ADD30 pKa = 3.62EE31 pKa = 5.01SAEE34 pKa = 4.16SYY36 pKa = 10.07PYY38 pKa = 11.37TMFAASNDD46 pKa = 3.71DD47 pKa = 3.57GAITINADD55 pKa = 3.38NFCVNGNVATNGTIVSSGNMNINGTKK81 pKa = 9.61TEE83 pKa = 4.19SADD86 pKa = 3.14EE87 pKa = 4.08SMIFIFDD94 pKa = 4.58KK95 pKa = 10.77IDD97 pKa = 3.1TKK99 pKa = 11.24YY100 pKa = 10.75FSVPNVEE107 pKa = 4.45EE108 pKa = 3.89HH109 pKa = 6.21TEE111 pKa = 4.08DD112 pKa = 3.61YY113 pKa = 10.95TIDD116 pKa = 3.63EE117 pKa = 4.69LNININVPTEE127 pKa = 4.14VQGEE131 pKa = 4.14ATLTGNININNALKK145 pKa = 10.82ALDD148 pKa = 4.02DD149 pKa = 3.96VNLYY153 pKa = 11.32GEE155 pKa = 4.55VKK157 pKa = 9.23NTNDD161 pKa = 3.57SIIFSKK167 pKa = 11.17YY168 pKa = 8.81GDD170 pKa = 3.39IVIDD174 pKa = 3.8SQNVNLNGLIYY185 pKa = 10.62APFGSVTINAQNLNLNNVVIIAEE208 pKa = 4.62SIVLTCPNVNANNGSNASSFVGTSSEE234 pKa = 4.18TFIAPIEE241 pKa = 3.93EE242 pKa = 3.95WQYY245 pKa = 11.85LNDD248 pKa = 3.78NDD250 pKa = 4.35EE251 pKa = 5.11DD252 pKa = 4.78GIPDD256 pKa = 3.72LVEE259 pKa = 3.86NAIGTDD265 pKa = 3.41NDD267 pKa = 4.04SQDD270 pKa = 3.4SDD272 pKa = 3.64GDD274 pKa = 4.06NIPDD278 pKa = 3.37YY279 pKa = 11.1TEE281 pKa = 4.24YY282 pKa = 11.29VVLNTDD288 pKa = 3.19PTSPNDD294 pKa = 3.37VMPFIQAAYY303 pKa = 10.03DD304 pKa = 3.52NLEE307 pKa = 3.99IEE309 pKa = 4.48LAIGDD314 pKa = 4.05TYY316 pKa = 11.97DD317 pKa = 3.3NVNNDD322 pKa = 2.47VVLPLKK328 pKa = 9.67STYY331 pKa = 10.51DD332 pKa = 3.4AVVEE336 pKa = 4.22WEE338 pKa = 4.08VSNDD342 pKa = 2.91EE343 pKa = 4.31VININGHH350 pKa = 5.57ISDD353 pKa = 4.16NLEE356 pKa = 3.95SSSEE360 pKa = 3.96VTYY363 pKa = 9.48TATIFLFGEE372 pKa = 4.07QLTKK376 pKa = 10.85KK377 pKa = 10.33FDD379 pKa = 3.38LHH381 pKa = 6.56VNPKK385 pKa = 10.07ASHH388 pKa = 6.62IDD390 pKa = 2.96IDD392 pKa = 4.41SIKK395 pKa = 10.87DD396 pKa = 3.46LTVDD400 pKa = 5.31DD401 pKa = 4.41IEE403 pKa = 4.83EE404 pKa = 4.19MNSDD408 pKa = 4.08DD409 pKa = 4.31EE410 pKa = 4.76DD411 pKa = 4.61FEE413 pKa = 5.86IEE415 pKa = 4.11INDD418 pKa = 3.36YY419 pKa = 11.26GYY421 pKa = 10.27IEE423 pKa = 5.08EE424 pKa = 4.68IFGKK428 pKa = 10.91YY429 pKa = 9.65SDD431 pKa = 4.61NSIKK435 pKa = 10.85SPNDD439 pKa = 3.27ALYY442 pKa = 10.9SLYY445 pKa = 10.63SIKK448 pKa = 9.79TALGISDD455 pKa = 4.06PFEE458 pKa = 3.89EE459 pKa = 5.25LKK461 pKa = 11.23LFDD464 pKa = 4.05IDD466 pKa = 3.93KK467 pKa = 10.96DD468 pKa = 3.87EE469 pKa = 4.52TGTIYY474 pKa = 11.1KK475 pKa = 7.99FTQLVNGVPCFDD487 pKa = 3.85NNIIVSCDD495 pKa = 3.2EE496 pKa = 4.49NGTTDD501 pKa = 3.77YY502 pKa = 11.07LRR504 pKa = 11.84SSYY507 pKa = 10.67FPVNTTISTSPNISYY522 pKa = 11.02EE523 pKa = 4.34DD524 pKa = 3.56INDD527 pKa = 5.23DD528 pKa = 3.93ILKK531 pKa = 9.33EE532 pKa = 4.05YY533 pKa = 9.76PDD535 pKa = 3.86ASIIDD540 pKa = 3.53VDD542 pKa = 3.51EE543 pKa = 4.81DD544 pKa = 3.38NRR546 pKa = 11.84LFILNYY552 pKa = 9.22YY553 pKa = 9.45GKK555 pKa = 10.9LYY557 pKa = 10.58LVWNKK562 pKa = 10.6YY563 pKa = 10.05IGVNNYY569 pKa = 8.05TYY571 pKa = 10.7QILVNANDD579 pKa = 3.88GKK581 pKa = 10.61ILYY584 pKa = 10.3KK585 pKa = 10.65NISDD589 pKa = 3.78INFDD593 pKa = 3.72EE594 pKa = 5.43DD595 pKa = 3.96LTITQDD601 pKa = 4.06DD602 pKa = 4.32LLKK605 pKa = 10.34QSRR608 pKa = 11.84TISILKK614 pKa = 9.53QNNLFVDD621 pKa = 4.17DD622 pKa = 4.53YY623 pKa = 11.52YY624 pKa = 11.84YY625 pKa = 11.36LEE627 pKa = 5.11DD628 pKa = 3.47SDD630 pKa = 5.72RR631 pKa = 11.84NIIVYY636 pKa = 9.49DD637 pKa = 3.97AQGKK641 pKa = 10.35AYY643 pKa = 10.22GSSDD647 pKa = 2.74KK648 pKa = 11.36KK649 pKa = 10.82NIKK652 pKa = 9.52KK653 pKa = 9.9HH654 pKa = 5.65LFLFIIILVHH664 pKa = 6.67HH665 pKa = 6.87GLLKK669 pKa = 10.49KK670 pKa = 10.64YY671 pKa = 10.18LLWQTWKK678 pKa = 10.07IYY680 pKa = 9.86MIFTITNSVDD690 pKa = 3.9FLLMMLGLKK699 pKa = 7.52QQEE702 pKa = 4.54MII704 pKa = 4.28

Molecular weight: 78.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011UF00|A0A011UF00_RUMAL 50S ribosomal protein L16 OS=Ruminococcus albus SY3 OX=1341156 GN=rplP PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.65QPKK8 pKa = 9.08KK9 pKa = 8.13LQRR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.7VHH16 pKa = 5.82GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.31

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3322

0

3322

1072678

37

3489

322.9

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.562 ± 0.044

1.693 ± 0.02

6.697 ± 0.032

7.222 ± 0.052

4.245 ± 0.028

7.04 ± 0.045

1.6 ± 0.019

7.26 ± 0.038

7.037 ± 0.038

8.089 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.897 ± 0.025

4.749 ± 0.037

3.201 ± 0.025

2.451 ± 0.022

4.173 ± 0.038

6.421 ± 0.049

5.822 ± 0.054

6.664 ± 0.035

0.892 ± 0.016

4.286 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski