Shewanella sp. phage 3/49

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088C4F7|A0A088C4F7_9CAUD Uncharacterized protein OS=Shewanella sp. phage 3/49 OX=1458863 GN=S349_56 PE=4 SV=1
MM1 pKa = 7.61TIDD4 pKa = 4.17IDD6 pKa = 4.14YY7 pKa = 11.04QDD9 pKa = 3.92VEE11 pKa = 5.18AIAAMRR17 pKa = 11.84YY18 pKa = 9.88GSLDD22 pKa = 3.34DD23 pKa = 5.33AAFADD28 pKa = 3.5AWLVMSHH35 pKa = 6.9NIYY38 pKa = 11.08ASMIIAADD46 pKa = 3.73VVDD49 pKa = 5.12DD50 pKa = 3.94FLNRR54 pKa = 11.84TVV56 pKa = 3.3

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088C543|A0A088C543_9CAUD Uncharacterized protein OS=Shewanella sp. phage 3/49 OX=1458863 GN=S349_17 PE=4 SV=1
MM1 pKa = 7.21YY2 pKa = 10.29CNNQAKK8 pKa = 9.47KK9 pKa = 9.95QAAFYY14 pKa = 10.77HH15 pKa = 5.34NQQLDD20 pKa = 3.52YY21 pKa = 11.39DD22 pKa = 4.07RR23 pKa = 11.84WLMDD27 pKa = 2.99NTRR30 pKa = 11.84NQQRR34 pKa = 11.84QRR36 pKa = 11.84WIAPAKK42 pKa = 8.24TRR44 pKa = 11.84KK45 pKa = 9.73ALATILAMSAGFAQIYY61 pKa = 9.27

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12964

47

848

185.2

20.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.002 ± 0.549

1.496 ± 0.174

6.464 ± 0.209

6.109 ± 0.281

3.973 ± 0.179

6.757 ± 0.407

1.635 ± 0.173

6.503 ± 0.23

6.773 ± 0.428

7.505 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.8 ± 0.177

5.546 ± 0.225

3.132 ± 0.193

3.664 ± 0.212

4.196 ± 0.198

7.266 ± 0.34

5.978 ± 0.257

6.549 ± 0.288

1.257 ± 0.138

3.394 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski