Micrococcales bacterium KH10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; unclassified Micrococcales

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2348 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239GDC6|A0A239GDC6_9MICO Histidine kinase OS=Micrococcales bacterium KH10 OX=1945885 GN=SAMN06309944_1268 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84IKK5 pKa = 11.05NPFSVNRR12 pKa = 11.84VATASIATGLALVLTACGGGTDD34 pKa = 3.93SPTPNDD40 pKa = 2.96VATNTEE46 pKa = 4.32GQSEE50 pKa = 4.35EE51 pKa = 4.08LSGEE55 pKa = 4.27VTLIAHH61 pKa = 7.43DD62 pKa = 4.14SFTFPDD68 pKa = 3.54EE69 pKa = 4.8LIADD73 pKa = 4.49FEE75 pKa = 4.8EE76 pKa = 4.76KK77 pKa = 9.97TGLTLNVTTTGDD89 pKa = 3.37GGQLANQLVLTKK101 pKa = 10.38DD102 pKa = 3.29APLADD107 pKa = 3.58AFFGVDD113 pKa = 3.08NSFVTGLIEE122 pKa = 4.77AGAVDD127 pKa = 5.13PYY129 pKa = 10.62TPEE132 pKa = 3.98SLGEE136 pKa = 4.15SATEE140 pKa = 4.24FAADD144 pKa = 3.92DD145 pKa = 4.51AGSVTPVDD153 pKa = 3.11HH154 pKa = 7.59GYY156 pKa = 10.62VCFNADD162 pKa = 3.43DD163 pKa = 3.56AWFEE167 pKa = 4.46EE168 pKa = 4.4NGLAAPEE175 pKa = 4.16TLDD178 pKa = 4.83DD179 pKa = 4.51LTDD182 pKa = 3.25EE183 pKa = 4.97AYY185 pKa = 10.44RR186 pKa = 11.84GLTVVMDD193 pKa = 4.49PTASSPGAAMLFATVAKK210 pKa = 10.13FGEE213 pKa = 4.58DD214 pKa = 3.37GYY216 pKa = 11.37RR217 pKa = 11.84DD218 pKa = 3.74YY219 pKa = 9.1WTKK222 pKa = 11.21LLANDD227 pKa = 3.93TKK229 pKa = 10.99VVQGWSDD236 pKa = 3.06AYY238 pKa = 11.21YY239 pKa = 10.95VDD241 pKa = 4.67FSGGGEE247 pKa = 4.03DD248 pKa = 3.39GQYY251 pKa = 10.45PIVVSYY257 pKa = 8.33NTSPAYY263 pKa = 9.13TVAEE267 pKa = 4.56GSDD270 pKa = 3.35EE271 pKa = 4.32STTSVLLDD279 pKa = 3.17TCTRR283 pKa = 11.84QVEE286 pKa = 4.48YY287 pKa = 11.1AGVLAGAKK295 pKa = 9.78NPAGARR301 pKa = 11.84AVIDD305 pKa = 3.68YY306 pKa = 10.1LVSQDD311 pKa = 3.83FQDD314 pKa = 4.01TVAEE318 pKa = 4.13TMYY321 pKa = 9.27MYY323 pKa = 10.57PVLPSGAVPDD333 pKa = 4.43EE334 pKa = 4.18WQNWAPVPQHH344 pKa = 6.64SLDD347 pKa = 3.86LAPDD351 pKa = 3.56QITANRR357 pKa = 11.84AAWLDD362 pKa = 3.44EE363 pKa = 3.79WSQLAGG369 pKa = 3.45

Molecular weight: 39.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239E1X9|A0A239E1X9_9MICO Carbamoyl-phosphate synthase small chain OS=Micrococcales bacterium KH10 OX=1945885 GN=carA PE=3 SV=1
AA1 pKa = 7.79RR2 pKa = 11.84GLRR5 pKa = 11.84VVKK8 pKa = 10.46ARR10 pKa = 11.84PKK12 pKa = 10.23KK13 pKa = 8.36VTVRR17 pKa = 11.84TKK19 pKa = 8.73QVRR22 pKa = 11.84SGASVKK28 pKa = 10.24VRR30 pKa = 11.84VKK32 pKa = 10.25VAKK35 pKa = 10.36LNNGRR40 pKa = 11.84HH41 pKa = 5.14ANGRR45 pKa = 11.84VQIRR49 pKa = 11.84IGKK52 pKa = 8.94RR53 pKa = 11.84VIGSVRR59 pKa = 11.84IKK61 pKa = 10.57PKK63 pKa = 10.44AKK65 pKa = 9.89GNRR68 pKa = 11.84TVTVKK73 pKa = 10.67LSSAQRR79 pKa = 11.84SGSSLKK85 pKa = 10.27VRR87 pKa = 11.84AKK89 pKa = 10.32FVAKK93 pKa = 10.38NNKK96 pKa = 8.4SFKK99 pKa = 9.8NRR101 pKa = 11.84QSKK104 pKa = 7.91VTTIRR109 pKa = 11.84LVRR112 pKa = 3.88

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2348

0

2348

839289

37

3203

357.4

38.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.13 ± 0.06

0.565 ± 0.012

6.288 ± 0.041

5.515 ± 0.052

2.831 ± 0.025

8.373 ± 0.043

2.033 ± 0.022

4.832 ± 0.034

2.827 ± 0.053

9.246 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.809 ± 0.018

2.691 ± 0.041

5.15 ± 0.038

3.551 ± 0.026

6.736 ± 0.053

6.205 ± 0.042

6.882 ± 0.064

8.711 ± 0.042

1.47 ± 0.022

2.156 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski