Microviridae Bog9017_22

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UK20|A0A0G2UK20_9VIRU DNA pilot protein VP2 OS=Microviridae Bog9017_22 OX=1655651 PE=4 SV=1
MM1 pKa = 7.08STQKK5 pKa = 10.36IIQTGKK11 pKa = 9.05VGLVSRR17 pKa = 11.84PNNSVPSNTFDD28 pKa = 3.87LSYY31 pKa = 11.11DD32 pKa = 3.33VKK34 pKa = 10.05TSFNIGEE41 pKa = 5.28LIPIGCWEE49 pKa = 4.74ALPGDD54 pKa = 4.05RR55 pKa = 11.84FNCQSEE61 pKa = 4.2ALVRR65 pKa = 11.84FAPMLAPIMQRR76 pKa = 11.84LNLRR80 pKa = 11.84LEE82 pKa = 4.22YY83 pKa = 10.6FFVPNRR89 pKa = 11.84ILWSDD94 pKa = 3.14WEE96 pKa = 4.55DD97 pKa = 4.2FISPSTEE104 pKa = 3.9GEE106 pKa = 4.07ASPSMAAFSDD116 pKa = 3.91DD117 pKa = 3.95FQVTPYY123 pKa = 10.5SVSGYY128 pKa = 10.57LGLPLTGPDD137 pKa = 3.73LVISKK142 pKa = 10.87DD143 pKa = 3.54DD144 pKa = 3.92VNCLRR149 pKa = 11.84HH150 pKa = 5.4AGYY153 pKa = 10.35RR154 pKa = 11.84RR155 pKa = 11.84IYY157 pKa = 9.05EE158 pKa = 3.55DD159 pKa = 3.07WYY161 pKa = 10.94RR162 pKa = 11.84DD163 pKa = 3.31EE164 pKa = 5.26NYY166 pKa = 11.13DD167 pKa = 3.4MSGGNTSLSSGFTNQFDD184 pKa = 3.84IYY186 pKa = 11.32DD187 pKa = 4.12HH188 pKa = 6.8IFTRR192 pKa = 11.84NLDD195 pKa = 3.22RR196 pKa = 11.84DD197 pKa = 4.38YY198 pKa = 10.66FTSSYY203 pKa = 10.46PWPQKK208 pKa = 10.64GADD211 pKa = 3.38VLIPVTIDD219 pKa = 2.9QTPQVVPVSRR229 pKa = 11.84TSQGVYY235 pKa = 8.59TVFEE239 pKa = 4.25PVDD242 pKa = 3.67NADD245 pKa = 3.52HH246 pKa = 7.01SGGAVDD252 pKa = 4.34WVPDD256 pKa = 4.32PEE258 pKa = 4.81PSSNTGYY265 pKa = 8.77LTAGSTPLSIDD276 pKa = 3.41PGSSGFQVSIPADD289 pKa = 3.43SSTVGTINDD298 pKa = 3.96LRR300 pKa = 11.84TAAAVQRR307 pKa = 11.84WLEE310 pKa = 3.57ADD312 pKa = 3.11IYY314 pKa = 7.5GTRR317 pKa = 11.84YY318 pKa = 9.45IEE320 pKa = 5.0LIEE323 pKa = 4.01NHH325 pKa = 6.25FATTVEE331 pKa = 4.02DD332 pKa = 4.25FRR334 pKa = 11.84LQRR337 pKa = 11.84AEE339 pKa = 3.95FLGMTLEE346 pKa = 5.26PIQISEE352 pKa = 4.26VLQTSEE358 pKa = 4.53TATSPQGNMSGHH370 pKa = 6.24GLAVHH375 pKa = 6.77KK376 pKa = 10.68GEE378 pKa = 4.99HH379 pKa = 5.91YY380 pKa = 10.57NYY382 pKa = 8.8HH383 pKa = 6.27CKK385 pKa = 10.05EE386 pKa = 3.92HH387 pKa = 5.43GHH389 pKa = 6.28FYY391 pKa = 10.85VIASVLPTNAYY402 pKa = 7.72YY403 pKa = 10.42QGVARR408 pKa = 11.84GFSRR412 pKa = 11.84FDD414 pKa = 3.36RR415 pKa = 11.84LDD417 pKa = 3.54FAWPEE422 pKa = 3.68LANIGFQEE430 pKa = 4.35LLNKK434 pKa = 9.94EE435 pKa = 4.16LFYY438 pKa = 11.29DD439 pKa = 4.71VSDD442 pKa = 4.61DD443 pKa = 4.27LNGDD447 pKa = 3.39VFGYY451 pKa = 8.88VPRR454 pKa = 11.84YY455 pKa = 8.93NEE457 pKa = 3.65YY458 pKa = 8.67RR459 pKa = 11.84TMYY462 pKa = 10.07SQVTGEE468 pKa = 4.13FATNLQYY475 pKa = 11.06WHH477 pKa = 7.13MGRR480 pKa = 11.84DD481 pKa = 3.61FASRR485 pKa = 11.84PTHH488 pKa = 5.22TEE490 pKa = 3.53PFNTTDD496 pKa = 3.33PSTVDD501 pKa = 2.77RR502 pKa = 11.84VFAVVEE508 pKa = 4.39STVSHH513 pKa = 6.62LYY515 pKa = 9.12VHH517 pKa = 7.31AFHH520 pKa = 6.94KK521 pKa = 10.79CKK523 pKa = 10.59VNRR526 pKa = 11.84KK527 pKa = 8.58LPRR530 pKa = 11.84FSVPYY535 pKa = 9.92LHH537 pKa = 7.4

Molecular weight: 60.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UMD9|A0A0G2UMD9_9VIRU Uncharacterized protein OS=Microviridae Bog9017_22 OX=1655651 PE=4 SV=1
MM1 pKa = 7.54NFTFQNVANWRR12 pKa = 11.84LLLNRR17 pKa = 11.84RR18 pKa = 11.84QTWPPSPVQPNLALSIKK35 pKa = 10.16DD36 pKa = 2.98IYY38 pKa = 11.39SRR40 pKa = 11.84FLRR43 pKa = 11.84RR44 pKa = 11.84QPLPDD49 pKa = 3.42VYY51 pKa = 11.17DD52 pKa = 4.22GFSSPDD58 pKa = 3.57DD59 pKa = 4.35LSDD62 pKa = 3.74FDD64 pKa = 4.62NLSKK68 pKa = 10.08IDD70 pKa = 3.27KK71 pKa = 10.14AAAAKK76 pKa = 10.11HH77 pKa = 4.39YY78 pKa = 9.65AKK80 pKa = 10.31RR81 pKa = 11.84VATLDD86 pKa = 3.28SDD88 pKa = 3.94LKK90 pKa = 11.03EE91 pKa = 3.88RR92 pKa = 11.84SKK94 pKa = 11.55KK95 pKa = 10.23NAFEE99 pKa = 3.82QAAARR104 pKa = 11.84HH105 pKa = 5.66RR106 pKa = 11.84KK107 pKa = 8.94SVADD111 pKa = 3.92EE112 pKa = 4.17VAKK115 pKa = 10.65EE116 pKa = 3.75LAKK119 pKa = 10.33TKK121 pKa = 10.08TVVSS125 pKa = 4.11

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1291

125

537

322.8

36.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.985 ± 2.034

1.084 ± 0.5

6.197 ± 0.632

3.796 ± 0.689

4.493 ± 0.887

5.887 ± 0.955

2.479 ± 0.899

4.105 ± 0.319

4.183 ± 1.254

8.753 ± 0.86

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.169 ± 0.586

5.19 ± 0.814

5.422 ± 0.472

5.345 ± 1.207

5.422 ± 0.994

8.366 ± 0.223

5.577 ± 0.729

6.739 ± 0.634

1.162 ± 0.281

4.648 ± 1.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski