Microbacterium agarici

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A9DVY7|A0A2A9DVY7_9MICO Carbohydrate ABC transporter substrate-binding protein (CUT1 family) OS=Microbacterium agarici OX=630514 GN=ATJ78_1459 PE=4 SV=1
MM1 pKa = 7.11PAARR5 pKa = 11.84RR6 pKa = 11.84VALVSTLAVLGTALIAPTAANAATPPGGVEE36 pKa = 3.99LTLLATTDD44 pKa = 3.63THH46 pKa = 6.24GHH48 pKa = 4.99VADD51 pKa = 3.51WDD53 pKa = 4.11YY54 pKa = 11.19FQNAPYY60 pKa = 8.67PTDD63 pKa = 3.98DD64 pKa = 3.43SLGLSRR70 pKa = 11.84VASVVDD76 pKa = 3.73DD77 pKa = 3.75VRR79 pKa = 11.84AEE81 pKa = 4.02KK82 pKa = 10.67GDD84 pKa = 3.62EE85 pKa = 4.25SVVVLDD91 pKa = 5.03NGDD94 pKa = 4.63AIQGTPLTYY103 pKa = 10.32LYY105 pKa = 10.78GYY107 pKa = 9.96GDD109 pKa = 3.65SRR111 pKa = 11.84DD112 pKa = 3.58SVLNDD117 pKa = 3.9TIEE120 pKa = 4.61HH121 pKa = 6.48PMAQVFDD128 pKa = 4.5TIGYY132 pKa = 7.71DD133 pKa = 3.31AQVVGNHH140 pKa = 5.97EE141 pKa = 4.32YY142 pKa = 11.02NYY144 pKa = 11.25GLDD147 pKa = 3.66LLQAYY152 pKa = 10.09DD153 pKa = 4.3EE154 pKa = 4.84DD155 pKa = 5.43LEE157 pKa = 5.7APLLGANVIDD167 pKa = 4.33AATGEE172 pKa = 4.8PYY174 pKa = 10.24HH175 pKa = 7.3DD176 pKa = 5.51PYY178 pKa = 11.41TLIDD182 pKa = 3.68RR183 pKa = 11.84EE184 pKa = 4.01IDD186 pKa = 3.49GKK188 pKa = 10.46QVTIGVIGLVTPGVRR203 pKa = 11.84IWDD206 pKa = 3.76KK207 pKa = 10.65QHH209 pKa = 6.82VEE211 pKa = 4.72GILEE215 pKa = 4.09FQDD218 pKa = 3.18MVEE221 pKa = 4.6AAKK224 pKa = 10.34QWVPEE229 pKa = 3.83VDD231 pKa = 3.24AQADD235 pKa = 4.03VVVVLAHH242 pKa = 6.55TGQGTVPDD250 pKa = 4.57DD251 pKa = 4.6AYY253 pKa = 11.22DD254 pKa = 3.98PADD257 pKa = 4.02LNEE260 pKa = 4.56DD261 pKa = 3.55VANNIAFQVPGIDD274 pKa = 3.46VLVAGHH280 pKa = 5.82SHH282 pKa = 5.7QDD284 pKa = 3.25EE285 pKa = 4.6PQTLITNTAGEE296 pKa = 4.37QTLITQPNYY305 pKa = 7.76WARR308 pKa = 11.84SVSEE312 pKa = 4.34VTLSLAPTADD322 pKa = 3.21GFEE325 pKa = 4.43VDD327 pKa = 3.88WSDD330 pKa = 3.47GYY332 pKa = 11.81APVVTPHH339 pKa = 6.59YY340 pKa = 9.11GTDD343 pKa = 3.68GTPEE347 pKa = 4.29DD348 pKa = 4.05PQVLEE353 pKa = 4.77VISEE357 pKa = 4.03EE358 pKa = 4.1HH359 pKa = 6.45EE360 pKa = 3.99ATIEE364 pKa = 4.08YY365 pKa = 10.73VNTPVADD372 pKa = 3.85SVEE375 pKa = 4.24EE376 pKa = 4.12LPAATSRR383 pKa = 11.84YY384 pKa = 9.07EE385 pKa = 3.75DD386 pKa = 3.32TAIIDD391 pKa = 4.57FINSIQLEE399 pKa = 4.56TVDD402 pKa = 3.89AALDD406 pKa = 3.61GTEE409 pKa = 4.13YY410 pKa = 11.41ADD412 pKa = 4.01VPVISQASPFSRR424 pKa = 11.84TALFPEE430 pKa = 4.85GEE432 pKa = 4.2VTIRR436 pKa = 11.84DD437 pKa = 3.33IAGLYY442 pKa = 9.73IYY444 pKa = 10.15EE445 pKa = 4.12NTLVGVQMTGAEE457 pKa = 3.62IAEE460 pKa = 4.09YY461 pKa = 10.73LEE463 pKa = 3.87YY464 pKa = 10.19SARR467 pKa = 11.84YY468 pKa = 8.18FVQTEE473 pKa = 4.13PGAEE477 pKa = 3.83FDD479 pKa = 4.32PATGTNAVYY488 pKa = 10.01PDD490 pKa = 4.36RR491 pKa = 11.84PDD493 pKa = 3.43GVPDD497 pKa = 3.47YY498 pKa = 10.99NYY500 pKa = 11.18DD501 pKa = 3.51VLTGLSYY508 pKa = 10.7TIDD511 pKa = 3.01ISQPVGEE518 pKa = 5.25RR519 pKa = 11.84ILDD522 pKa = 3.82LAHH525 pKa = 7.63PDD527 pKa = 3.82GTPVAADD534 pKa = 3.52DD535 pKa = 4.21TFVLAVNNYY544 pKa = 7.8RR545 pKa = 11.84QSGGGAFPNVADD557 pKa = 4.12APVVYY562 pKa = 10.08DD563 pKa = 3.46EE564 pKa = 4.66RR565 pKa = 11.84LEE567 pKa = 3.89IRR569 pKa = 11.84QLLIDD574 pKa = 3.72WASEE578 pKa = 3.76RR579 pKa = 11.84GTIDD583 pKa = 3.18PADD586 pKa = 3.57FFVEE590 pKa = 3.95NWEE593 pKa = 4.28LTTDD597 pKa = 3.75AATEE601 pKa = 4.45TPTPTPEE608 pKa = 3.92PTDD611 pKa = 4.03PGEE614 pKa = 4.49GDD616 pKa = 4.1DD617 pKa = 5.4PGAGDD622 pKa = 5.43DD623 pKa = 4.72GSTDD627 pKa = 3.49APGTGSDD634 pKa = 3.71GAANDD639 pKa = 4.81DD640 pKa = 4.01SSTDD644 pKa = 3.46AASDD648 pKa = 4.0LPVTGADD655 pKa = 3.38IAWPLGLAAALLLLGAGLVATRR677 pKa = 11.84RR678 pKa = 11.84FARR681 pKa = 11.84KK682 pKa = 9.38RR683 pKa = 11.84SS684 pKa = 3.44

Molecular weight: 72.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A9DTK4|A0A2A9DTK4_9MICO Uncharacterized protein OS=Microbacterium agarici OX=630514 GN=ATJ78_0381 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3074

0

3074

989917

29

2145

322.0

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.632 ± 0.059

0.548 ± 0.011

6.48 ± 0.043

5.84 ± 0.045

3.182 ± 0.026

8.484 ± 0.042

2.059 ± 0.021

4.969 ± 0.034

2.237 ± 0.032

9.75 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.019

2.256 ± 0.021

4.996 ± 0.027

2.868 ± 0.021

6.998 ± 0.05

6.288 ± 0.032

6.145 ± 0.033

8.832 ± 0.039

1.422 ± 0.018

2.024 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski