Colwellia sp. RSH04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Colwellia; unclassified Colwellia

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396TYR6|A0A396TYR6_9GAMM Biopolymer transporter ExbD OS=Colwellia sp. RSH04 OX=2305464 GN=D1094_08795 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.54NKK4 pKa = 9.72AQQGFTLIEE13 pKa = 4.08LVVVIVILGILAVTAAPKK31 pKa = 10.27FINLQADD38 pKa = 3.16ARR40 pKa = 11.84TATLEE45 pKa = 3.92AVKK48 pKa = 10.83ASMQSAYY55 pKa = 9.8TLVHH59 pKa = 6.29SKK61 pKa = 10.98SLIQGNEE68 pKa = 3.77SAVAADD74 pKa = 3.9GEE76 pKa = 5.04TVVVNSQGDD85 pKa = 4.12VVNLDD90 pKa = 3.36LGYY93 pKa = 9.85PISTAGAATVPADD106 pKa = 3.56DD107 pKa = 3.68WALLLEE113 pKa = 4.48VNADD117 pKa = 3.51EE118 pKa = 6.48FIISDD123 pKa = 4.01DD124 pKa = 4.04SIAGYY129 pKa = 9.68VVVYY133 pKa = 9.36PEE135 pKa = 5.15GGTEE139 pKa = 4.0PTALPADD146 pKa = 3.86NDD148 pKa = 3.74APTAAEE154 pKa = 4.31SSCFAYY160 pKa = 10.52YY161 pKa = 10.08QEE163 pKa = 4.33SQALGTSPVTDD174 pKa = 3.36VVICLL179 pKa = 3.67

Molecular weight: 18.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396TWV7|A0A396TWV7_9GAMM PEP-CTERM system TPR-repeat protein PrsT OS=Colwellia sp. RSH04 OX=2305464 GN=prsT PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3551

0

3551

1191842

31

2560

335.6

37.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.029 ± 0.037

1.001 ± 0.012

5.472 ± 0.039

6.223 ± 0.033

4.362 ± 0.026

6.21 ± 0.043

2.194 ± 0.017

7.104 ± 0.032

6.25 ± 0.044

10.2 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.019

5.183 ± 0.033

3.472 ± 0.02

4.685 ± 0.037

3.724 ± 0.026

7.115 ± 0.035

5.531 ± 0.036

6.57 ± 0.034

1.123 ± 0.015

3.217 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski