Clostridium sp. CAG:571

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5XJW7|R5XJW7_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:571 OX=1262822 GN=BN716_00572 PE=4 SV=1
MM1 pKa = 7.73EE2 pKa = 5.41EE3 pKa = 3.88DD4 pKa = 3.16CGYY7 pKa = 10.4VQDD10 pKa = 3.85VCADD14 pKa = 4.01LEE16 pKa = 4.53SGRR19 pKa = 11.84ITSIIVPGNNKK30 pKa = 8.83ILNMFTGSNDD40 pKa = 4.69IVIQWQNIKK49 pKa = 10.74CIGDD53 pKa = 3.65DD54 pKa = 3.94VILVEE59 pKa = 4.38II60 pKa = 4.72

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5XNW7|R5XNW7_9CLOT 30S ribosomal protein S9 OS=Clostridium sp. CAG:571 OX=1262822 GN=rpsI PE=3 SV=1
MM1 pKa = 6.67QSEE4 pKa = 4.57YY5 pKa = 10.69FKK7 pKa = 10.62PIEE10 pKa = 4.08YY11 pKa = 10.83KK12 pKa = 10.29MLDD15 pKa = 3.38KK16 pKa = 10.91KK17 pKa = 10.53IAYY20 pKa = 7.76QGKK23 pKa = 8.22RR24 pKa = 11.84ISVEE28 pKa = 3.6EE29 pKa = 3.88VNYY32 pKa = 10.97LNGNQKK38 pKa = 9.55IYY40 pKa = 10.14RR41 pKa = 11.84EE42 pKa = 4.03HH43 pKa = 7.86VIAGDD48 pKa = 3.52AAVILAIKK56 pKa = 10.54DD57 pKa = 3.42NGNVIMIQEE66 pKa = 4.31PRR68 pKa = 11.84TPINQVILGLPARR81 pKa = 11.84TNRR84 pKa = 11.84EE85 pKa = 3.26GRR87 pKa = 11.84KK88 pKa = 8.87RR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84WCKK94 pKa = 9.43KK95 pKa = 7.39RR96 pKa = 11.84ARR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84NRR102 pKa = 11.84LLCKK106 pKa = 10.04KK107 pKa = 9.18NRR109 pKa = 11.84KK110 pKa = 8.11IKK112 pKa = 10.38RR113 pKa = 11.84VLPFSGIYY121 pKa = 9.34KK122 pKa = 10.35

Molecular weight: 14.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1620

0

1620

436713

29

3489

269.6

30.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.772 ± 0.055

1.164 ± 0.026

5.532 ± 0.049

8.201 ± 0.078

4.04 ± 0.051

5.47 ± 0.058

1.149 ± 0.022

10.573 ± 0.084

10.655 ± 0.071

8.33 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.03

7.348 ± 0.075

2.407 ± 0.032

2.528 ± 0.034

3.218 ± 0.047

5.904 ± 0.056

5.17 ± 0.064

6.026 ± 0.062

0.621 ± 0.017

4.47 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski