Shewanella sp. phage 1/44

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088C3X5|A0A088C3X5_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/44 OX=1458862 GN=S144_26 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.43LTDD5 pKa = 5.31DD6 pKa = 3.78EE7 pKa = 5.24LSIIEE12 pKa = 4.06QGLYY16 pKa = 10.51AIEE19 pKa = 3.95QTPEE23 pKa = 3.9HH24 pKa = 6.47LGEE27 pKa = 4.38LATHH31 pKa = 6.62EE32 pKa = 4.76LLLGLDD38 pKa = 3.48VDD40 pKa = 3.79EE41 pKa = 5.93LYY43 pKa = 11.36YY44 pKa = 10.98HH45 pKa = 6.58LTNFEE50 pKa = 4.09LMSRR54 pKa = 11.84FEE56 pKa = 3.99QAQEE60 pKa = 3.96MVHH63 pKa = 7.06NDD65 pKa = 2.92INDD68 pKa = 3.37GMKK71 pKa = 9.6WGSIYY76 pKa = 10.51EE77 pKa = 4.08

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088C4A5|A0A088C4A5_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/44 OX=1458862 GN=S144_1 PE=4 SV=1
MM1 pKa = 7.81ALTFACVARR10 pKa = 11.84MAGAPGLRR18 pKa = 11.84YY19 pKa = 9.78SRR21 pKa = 11.84AYY23 pKa = 9.67QCGEE27 pKa = 3.92GTQTRR32 pKa = 11.84AKK34 pKa = 10.04RR35 pKa = 11.84KK36 pKa = 9.01LRR38 pKa = 11.84KK39 pKa = 9.44KK40 pKa = 9.83IKK42 pKa = 10.04KK43 pKa = 9.45

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15814

31

1401

210.9

23.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.145 ± 0.611

1.353 ± 0.175

6.456 ± 0.215

5.141 ± 0.42

3.573 ± 0.218

6.772 ± 0.255

1.859 ± 0.19

6.564 ± 0.234

6.09 ± 0.376

8.252 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.169

5.641 ± 0.261

3.971 ± 0.49

3.529 ± 0.242

4.243 ± 0.249

6.665 ± 0.309

6.924 ± 0.326

7.272 ± 0.307

1.277 ± 0.176

3.541 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski