uncultured bacterium

Taxonomy: cellular organisms; Bacteria; environmental samples

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090KNA6|A0A090KNA6_9BACT Uncultured bacterium genome assembly Metasoil_fosmids_resub OS=uncultured bacterium OX=77133 PE=4 SV=1
MM1 pKa = 7.13IRR3 pKa = 11.84APRR6 pKa = 11.84TRR8 pKa = 11.84RR9 pKa = 11.84SFNRR13 pKa = 11.84SRR15 pKa = 11.84LWLEE19 pKa = 3.74SLEE22 pKa = 4.27TRR24 pKa = 11.84VTPTTIPVSTLNDD37 pKa = 3.34MGQGSLRR44 pKa = 11.84QAIIDD49 pKa = 3.75ANFNPGPDD57 pKa = 3.47TIEE60 pKa = 3.83FTVAGTIQLTTGALPTITDD79 pKa = 3.61TVKK82 pKa = 10.21IDD84 pKa = 3.23GRR86 pKa = 11.84SAPGFLAAPVVEE98 pKa = 4.49IDD100 pKa = 3.09AHH102 pKa = 5.87GFAGLFFEE110 pKa = 5.09IGSNGSSLLSLAVGNANGAGVTLDD134 pKa = 3.84DD135 pKa = 4.91SNNNVAGNYY144 pKa = 9.32IGLALDD150 pKa = 4.55GSTTASNVGDD160 pKa = 3.42GLVINSSSINNIIGGTSPLDD180 pKa = 3.43RR181 pKa = 11.84NVISGNTGDD190 pKa = 4.86GITVSGSTRR199 pKa = 11.84NQIVGNFIGPDD210 pKa = 3.2ASGLGGRR217 pKa = 11.84GNGLDD222 pKa = 4.91GILLTAGAAVNNVGGTIDD240 pKa = 3.39AQRR243 pKa = 11.84NIISCNAADD252 pKa = 4.56GVSLEE257 pKa = 4.45QGAHH261 pKa = 6.43DD262 pKa = 5.68NIILGNFIGTDD273 pKa = 3.44VTGLAGLGNGLDD285 pKa = 4.0GVHH288 pKa = 7.23ADD290 pKa = 3.75DD291 pKa = 5.29AANNVIGNTVPVKK304 pKa = 10.46NVSYY308 pKa = 10.61FNANLVSPTPNPGWTGIRR326 pKa = 11.84ASDD329 pKa = 3.26TSGNYY334 pKa = 9.86LISGISGTQGLLYY347 pKa = 10.48EE348 pKa = 4.58GTISGAGVTIPVNFPNASASAVYY371 pKa = 10.47GPDD374 pKa = 3.22NLGGNHH380 pKa = 5.27VALVGSYY387 pKa = 10.36NVTGTGLADD396 pKa = 3.13QKK398 pKa = 11.65GFIFEE403 pKa = 4.2GTTADD408 pKa = 3.48LTNPANYY415 pKa = 7.33TTIDD419 pKa = 3.04AGGDD423 pKa = 3.49FNIVHH428 pKa = 6.08STMQGLAVGNSDD440 pKa = 3.63SSPSQGQASLLSHH453 pKa = 6.95AFLYY457 pKa = 10.7DD458 pKa = 3.42VASGQFLPDD467 pKa = 2.86IVFPGSVSNTAYY479 pKa = 10.49GIWFNGGTSYY489 pKa = 9.53TICGGWSSDD498 pKa = 3.41PVNNFDD504 pKa = 4.94DD505 pKa = 3.76QNRR508 pKa = 11.84PIGQGYY514 pKa = 8.82LVDD517 pKa = 4.35YY518 pKa = 11.02DD519 pKa = 4.8SLTQTFTNWTSFQYY533 pKa = 10.73PFGKK537 pKa = 10.28SFFTHH542 pKa = 6.54FEE544 pKa = 4.55GISSVEE550 pKa = 3.87NGVYY554 pKa = 9.5TLNADD559 pKa = 3.72SFQVGTTNPIQGSWVSVRR577 pKa = 11.84RR578 pKa = 11.84NSDD581 pKa = 2.78GSFSLGQWTNLAYY594 pKa = 9.88PGTGNITSSNSVYY607 pKa = 10.13GNQVVGVVFHH617 pKa = 6.86GATAFAFQATVNIGFQLSNVIGCNADD643 pKa = 3.7DD644 pKa = 4.7GVLLTGGATANTIASNFIGLDD665 pKa = 3.49LTGKK669 pKa = 10.14AALANGSDD677 pKa = 3.63GVEE680 pKa = 4.21VEE682 pKa = 4.11NSQGNLIGTTSTGPEE697 pKa = 3.39VNYY700 pKa = 10.31FNTSLITSVPVSGWEE715 pKa = 4.56GIRR718 pKa = 11.84QADD721 pKa = 3.98LPGQFLISGTSGSNGLLFEE740 pKa = 4.19GTIDD744 pKa = 4.62GVGTTNIVDD753 pKa = 3.64YY754 pKa = 9.98PGASSTFVYY763 pKa = 10.62GPDD766 pKa = 3.57NEE768 pKa = 4.73GSGNLGLVGTYY779 pKa = 7.92KK780 pKa = 10.07TANSSVVNGFLFQGTVADD798 pKa = 5.16LGTPANFQTINKK810 pKa = 9.71SGATFNYY817 pKa = 9.54LHH819 pKa = 6.28STMGGLIVGNYY830 pKa = 9.93DD831 pKa = 3.15SAPDD835 pKa = 3.72HH836 pKa = 6.18GQGGLPFGPGHH847 pKa = 5.64ATIYY851 pKa = 10.71DD852 pKa = 3.69IATQTYY858 pKa = 7.89LTDD861 pKa = 3.19IVFPGSKK868 pKa = 9.98SNSVYY873 pKa = 10.87GIWFNGGTSYY883 pKa = 9.4TVCGGYY889 pKa = 10.12SPTFVNNFDD898 pKa = 3.81DD899 pKa = 3.72QNRR902 pKa = 11.84PIGQAYY908 pKa = 9.02LANYY912 pKa = 7.69DD913 pKa = 3.65TATGQFSHH921 pKa = 6.68FTSFSYY927 pKa = 10.7PFGTNFLTHH936 pKa = 6.55FEE938 pKa = 4.92GISSVEE944 pKa = 3.78PGVYY948 pKa = 9.24TLAADD953 pKa = 4.9SIQSGSSQTIQASWVVVRR971 pKa = 11.84RR972 pKa = 11.84NPDD975 pKa = 2.68GSFGTGQWVNLDD987 pKa = 3.67YY988 pKa = 11.2PGVATTGTFTGSDD1001 pKa = 3.25SVYY1004 pKa = 9.18GNQVVGIVVSSTSTFCFQATVNVGTQMSNIISGNSGNGVLLNVGAQNNQVAGNFIGTDD1062 pKa = 3.36ATGSASLGNSLNGVQLEE1079 pKa = 4.37GANNNVIGNNNPITDD1094 pKa = 3.37IPFFNTDD1101 pKa = 3.04SVSVQVSGWQGIRR1114 pKa = 11.84NSDD1117 pKa = 3.3TTGQFLITGTSGTNGLLFDD1136 pKa = 3.87GTIAGVGTSYY1146 pKa = 10.87QVNFPNAATTSVYY1159 pKa = 10.81GPDD1162 pKa = 3.48NQGSGNIGLVGSYY1175 pKa = 10.4KK1176 pKa = 10.86NSDD1179 pKa = 3.22ADD1181 pKa = 3.74TAAVKK1186 pKa = 10.96VNGFAFQGTVADD1198 pKa = 4.98LGNSANYY1205 pKa = 7.14QTIDD1209 pKa = 2.93IAGAEE1214 pKa = 4.18FNYY1217 pKa = 9.98VHH1219 pKa = 6.08STMNGLAVGNYY1230 pKa = 9.93DD1231 pKa = 3.21SAADD1235 pKa = 3.65HH1236 pKa = 6.6GMGGLPLGPGHH1247 pKa = 7.33AFIYY1251 pKa = 10.35DD1252 pKa = 4.22LNQHH1256 pKa = 6.29KK1257 pKa = 10.0FLTDD1261 pKa = 2.7IMFPGSLSNTAYY1273 pKa = 10.51GIWFNGNDD1281 pKa = 3.62SYY1283 pKa = 10.45TICGGWSPDD1292 pKa = 3.82LVNNLLNQDD1301 pKa = 3.47QPIGKK1306 pKa = 9.9GYY1308 pKa = 10.46LVDD1311 pKa = 4.09YY1312 pKa = 10.36NSKK1315 pKa = 9.12TGAFTHH1321 pKa = 6.25FTSFDD1326 pKa = 3.7YY1327 pKa = 10.96PFGTNFVTHH1336 pKa = 6.46FEE1338 pKa = 4.8GISSVEE1344 pKa = 3.84KK1345 pKa = 10.65GVYY1348 pKa = 8.58TLSADD1353 pKa = 3.65SVQAGSNAPVQGSFVSVLRR1372 pKa = 11.84NTDD1375 pKa = 2.75GSFGSGQWINLNYY1388 pKa = 9.92PGLNPTVNITSSNSVYY1404 pKa = 10.7GNAVVGIVIGANPFSYY1420 pKa = 10.24QATVNVGFQLSNVISGNGGNGIEE1443 pKa = 4.6LDD1445 pKa = 3.77ASNGNQIAMNNVGTDD1460 pKa = 3.5LTGSRR1465 pKa = 11.84DD1466 pKa = 3.34LGNFGNGVLLTGASANNMVGGVATGGNAPTNGVFVRR1502 pKa = 11.84PPEE1505 pKa = 4.36GNLISGNDD1513 pKa = 3.46QNGVLITDD1521 pKa = 4.9LATHH1525 pKa = 6.16NQLSGNFIGTTAPGNSAMGNALDD1548 pKa = 5.78GVAIDD1553 pKa = 4.0NAPGNSLIGCTFQQDD1568 pKa = 3.3PFVFYY1573 pKa = 11.19NVIDD1577 pKa = 4.16GNGGNGLRR1585 pKa = 11.84VTNSDD1590 pKa = 2.82NTTFQANFLGLGADD1604 pKa = 3.27NSTPVGNALDD1614 pKa = 3.94GALINGSSANIQFGGVIPLGNVDD1637 pKa = 3.9AANGRR1642 pKa = 11.84NGVEE1646 pKa = 4.03IADD1649 pKa = 4.01TASGGVYY1656 pKa = 10.45FNTFCGLPAFVDD1668 pKa = 3.71TAVGNTLDD1676 pKa = 3.94GFLVTSTGGNNTIRR1690 pKa = 11.84TNVVSGNKK1698 pKa = 10.01GNGIHH1703 pKa = 7.0ISGNATGVQVTDD1715 pKa = 4.3TIVGLNTSGTTAIPNGANGILLDD1738 pKa = 5.36GNAHH1742 pKa = 6.94DD1743 pKa = 4.49NNIGGFQPSVIPEE1756 pKa = 3.64VTIAANGANGIAVVGNAKK1774 pKa = 10.5NNTIFHH1780 pKa = 6.21SLIGLNISGLTAIGNAGAGIFVGGAATGTIIGGTGPGQKK1819 pKa = 9.98VVVSGNLGGGIQLSGQSQGTQIIGSLIGTNKK1850 pKa = 10.45LGTAPVGNHH1859 pKa = 5.84GNGITIVSSGNSVGGTAAGQGNTVAFNSQFGIVVDD1894 pKa = 4.0TGTGNGILSNSIFNNGSGGILLTTGGNLGQPAPVLTFIPQTGSLTATVNGTLTAVANTTYY1954 pKa = 10.17TVQLFASSSAVPGGQGQFVVSSFSATTNGSGVASFSVTVTIPSSSDD2000 pKa = 3.06QFFTATATDD2009 pKa = 4.14PNKK2012 pKa = 8.72NTSMFSAAVQWTTPNAQYY2030 pKa = 9.04VTSAYY2035 pKa = 10.26QLLLGRR2041 pKa = 11.84MPDD2044 pKa = 3.07ASGGVFWVNQLNAGASPASVVLGIQGSSEE2073 pKa = 4.09YY2074 pKa = 10.34LTDD2077 pKa = 4.28QVFALYY2083 pKa = 10.48GRR2085 pKa = 11.84YY2086 pKa = 9.33LDD2088 pKa = 4.54RR2089 pKa = 11.84APEE2092 pKa = 4.1STGAEE2097 pKa = 3.91FWTNFLIAGGTFEE2110 pKa = 5.17QVAEE2114 pKa = 4.02QLISSPEE2121 pKa = 3.69YY2122 pKa = 10.03FQAHH2126 pKa = 6.71GATNAGFVLGLYY2138 pKa = 10.34ADD2140 pKa = 3.73VFGRR2144 pKa = 11.84TPSRR2148 pKa = 11.84FEE2150 pKa = 3.64LASWVIVLDD2159 pKa = 4.22AGASRR2164 pKa = 11.84LTVATDD2170 pKa = 3.93FLTSLEE2176 pKa = 3.97YY2177 pKa = 10.7RR2178 pKa = 11.84QDD2180 pKa = 3.81LVEE2183 pKa = 4.78SDD2185 pKa = 3.17YY2186 pKa = 11.39DD2187 pKa = 3.44IYY2189 pKa = 11.16LGRR2192 pKa = 11.84TADD2195 pKa = 3.67QNGLNFWVNALNTGSTDD2212 pKa = 3.47QEE2214 pKa = 4.06VLAGIFGSPEE2224 pKa = 3.8GFAKK2228 pKa = 10.46WSS2230 pKa = 3.39

Molecular weight: 228.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090KMN6|A0A090KMN6_9BACT Uncultured bacterium genome assembly Metasoil_fosmids_resub OS=uncultured bacterium OX=77133 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LGPGLSFSWRR12 pKa = 11.84RR13 pKa = 11.84AFGVSAFNARR23 pKa = 11.84IARR26 pKa = 11.84RR27 pKa = 11.84TGIPTTRR34 pKa = 11.84SGLDD38 pKa = 3.03RR39 pKa = 11.84KK40 pKa = 10.06IGRR43 pKa = 11.84FFTAGLLSLLFGGRR57 pKa = 11.84RR58 pKa = 3.39

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1289

1

1290

357591

21

2758

277.2

30.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.039 ± 0.109

1.015 ± 0.022

5.427 ± 0.041

5.61 ± 0.076

3.84 ± 0.053

8.015 ± 0.103

2.232 ± 0.033

5.051 ± 0.058

3.823 ± 0.062

9.965 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.033

2.986 ± 0.077

5.481 ± 0.07

3.461 ± 0.048

7.521 ± 0.099

5.791 ± 0.063

5.427 ± 0.068

7.235 ± 0.059

1.565 ± 0.032

2.435 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski