Flexilinea flocculi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; Flexilinea

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K8PBE2|A0A0K8PBE2_9CHLR Cysteine synthase OS=Flexilinea flocculi OX=1678840 GN=ATC1_12517 PE=4 SV=1
MM1 pKa = 7.54LWLFSDD7 pKa = 4.15IVQDD11 pKa = 4.54NIDD14 pKa = 4.27DD15 pKa = 3.92KK16 pKa = 11.22HH17 pKa = 7.15VKK19 pKa = 9.13YY20 pKa = 10.51YY21 pKa = 10.88YY22 pKa = 10.34YY23 pKa = 11.27GSTEE27 pKa = 3.77PDD29 pKa = 3.13NPEE32 pKa = 4.2NLDD35 pKa = 3.61PDD37 pKa = 3.56SWIEE41 pKa = 3.78TTYY44 pKa = 10.69YY45 pKa = 10.86GPISITITNDD55 pKa = 2.76VTNSQITFTNGASYY69 pKa = 10.28TPVSPTAGTTNQPIGGFSLQADD91 pKa = 4.55FPGASLTSVTISTTGTRR108 pKa = 11.84SGITNLKK115 pKa = 9.53LWSSTDD121 pKa = 3.01ATFNASSDD129 pKa = 3.63TLLNSQSDD137 pKa = 3.9NASVTFSGFSSTISTAGSYY156 pKa = 10.58YY157 pKa = 10.48FVTADD162 pKa = 4.18LSTSASGSIALTIGSVSNLTFSGGAIASGFTNAPLSSGTITIILIRR208 pKa = 11.84EE209 pKa = 3.9MNITGNGVTILNGDD223 pKa = 4.12TSPAASDD230 pKa = 3.37HH231 pKa = 6.37TDD233 pKa = 3.54FGSVDD238 pKa = 3.23IASGSVVHH246 pKa = 6.71TFTIQNNGAEE256 pKa = 4.42ALSLTGSSPFVAISGATADD275 pKa = 5.18FSLTQNPSSSIAGNSSTTFEE295 pKa = 4.03ITFNPASTGLKK306 pKa = 9.8SAVISIANDD315 pKa = 3.7DD316 pKa = 3.97GDD318 pKa = 4.18EE319 pKa = 4.1NPYY322 pKa = 9.89TFTIQGTGTAVPNMSVSGNAMQITNGDD349 pKa = 3.81IEE351 pKa = 4.5PSEE354 pKa = 5.06SDD356 pKa = 3.28DD357 pKa = 3.64TDD359 pKa = 3.78FGSVNIAGDD368 pKa = 4.27PIEE371 pKa = 4.35HH372 pKa = 6.78TFTIANNGSASLTLTGSSPYY392 pKa = 10.67VSLTGDD398 pKa = 3.56TEE400 pKa = 5.47DD401 pKa = 3.76FTLTQLPASNIAAGGGTTTFKK422 pKa = 9.92ITFKK426 pKa = 9.92PTATGNRR433 pKa = 11.84SATVSIANDD442 pKa = 3.89DD443 pKa = 3.92DD444 pKa = 4.34SKK446 pKa = 11.83NPFNFSIQGSGISVPTVTTAAASSVTKK473 pKa = 9.93TVATLGGNVTSDD485 pKa = 3.02GGSPITEE492 pKa = 4.01TGVVYY497 pKa = 10.61SSSNATPTIGGMDD510 pKa = 4.19VIQNANIDD518 pKa = 3.69LSGDD522 pKa = 3.44FSEE525 pKa = 5.52SISPLSSATHH535 pKa = 6.38YY536 pKa = 10.43YY537 pKa = 8.74FQAYY541 pKa = 6.59ATNAAGTSYY550 pKa = 11.18GGVLEE555 pKa = 4.38FTTLTSVVSILRR567 pKa = 11.84ADD569 pKa = 4.25PDD571 pKa = 3.71DD572 pKa = 3.95TNAASVRR579 pKa = 11.84WNVVFEE585 pKa = 4.42NNVTGLSASNFTLVSFGISGAAITGVSGSGTNWIVTASTGSGTGSLRR632 pKa = 11.84LGLVNDD638 pKa = 4.04NGLTPKK644 pKa = 10.56LSNPTLGGDD653 pKa = 3.91AYY655 pKa = 9.86TLDD658 pKa = 3.94LVSPSVTDD666 pKa = 3.32VDD668 pKa = 3.63TGKK671 pKa = 9.85PDD673 pKa = 3.05GTYY676 pKa = 10.99GVGSSIGIVIMFDD689 pKa = 3.09EE690 pKa = 4.4SHH692 pKa = 6.55NVDD695 pKa = 3.44GTPQLTLKK703 pKa = 8.88MDD705 pKa = 3.41SMNRR709 pKa = 11.84VIDD712 pKa = 4.74CISNEE717 pKa = 4.47HH718 pKa = 6.38IRR720 pKa = 11.84LICTYY725 pKa = 8.99TVQAGDD731 pKa = 3.56NTSDD735 pKa = 3.56LDD737 pKa = 4.12YY738 pKa = 11.62AGMTALSTNGGTIRR752 pKa = 11.84DD753 pKa = 3.79YY754 pKa = 11.22HH755 pKa = 7.8GNDD758 pKa = 4.57AILNLPWNAGTGSLAGNNAIVIDD781 pKa = 4.09TTPPEE786 pKa = 4.15ITIHH790 pKa = 6.26HH791 pKa = 7.19PGSSAAVSKK800 pKa = 9.77TITATTSDD808 pKa = 3.7GTIKK812 pKa = 10.23MSNTRR817 pKa = 11.84GTTCDD822 pKa = 2.97GSLIFTAYY830 pKa = 10.49ASQTFNAEE838 pKa = 3.55TDD840 pKa = 3.02NGIRR844 pKa = 11.84VCYY847 pKa = 9.75QAVDD851 pKa = 3.68TVNNISYY858 pKa = 10.4KK859 pKa = 10.63LSDD862 pKa = 5.22PISGIDD868 pKa = 3.3TTKK871 pKa = 9.28PTTSIIAKK879 pKa = 9.42PGNPDD884 pKa = 2.97NSASPSFTFSGEE896 pKa = 4.22DD897 pKa = 3.4PDD899 pKa = 4.5GTDD902 pKa = 3.65GSDD905 pKa = 3.15GSGIAGFEE913 pKa = 4.16CSLDD917 pKa = 3.6GEE919 pKa = 4.76PFSSCSSPKK928 pKa = 9.76TYY930 pKa = 10.92SSLGDD935 pKa = 3.6GNHH938 pKa = 5.92TFQVRR943 pKa = 11.84SVDD946 pKa = 3.45AAGNTDD952 pKa = 4.84DD953 pKa = 5.56SPEE956 pKa = 4.23SYY958 pKa = 8.6TWQIDD963 pKa = 3.37TSLPEE968 pKa = 4.05TTILSSPANPTASTDD983 pKa = 3.24AVFEE987 pKa = 4.27FEE989 pKa = 4.36GADD992 pKa = 3.43PGGSGVSGFEE1002 pKa = 4.05CSLDD1006 pKa = 3.55GEE1008 pKa = 4.6PFSDD1012 pKa = 3.81CSSPKK1017 pKa = 10.22SYY1019 pKa = 11.33SSLSDD1024 pKa = 3.39GNHH1027 pKa = 5.58TFLVRR1032 pKa = 11.84SVDD1035 pKa = 3.49AVGNADD1041 pKa = 3.55SSPAEE1046 pKa = 4.22FNWTVDD1052 pKa = 3.32TTPPEE1057 pKa = 4.07IAIHH1061 pKa = 6.17HH1062 pKa = 6.83PGSSAAVSKK1071 pKa = 9.77TITATTSDD1079 pKa = 3.7GTIKK1083 pKa = 10.23MSNTRR1088 pKa = 11.84GTTCDD1093 pKa = 3.06GSLTFTVYY1101 pKa = 10.91ASQTFNAEE1109 pKa = 3.55TDD1111 pKa = 3.02NGIRR1115 pKa = 11.84VCYY1118 pKa = 9.6QAVDD1122 pKa = 3.4AVNNISYY1129 pKa = 10.53KK1130 pKa = 10.7LSDD1133 pKa = 5.22PISGIDD1139 pKa = 3.3TTKK1142 pKa = 9.28PTTSIIAKK1150 pKa = 9.42PGNPDD1155 pKa = 2.97NSASPSFTFSGEE1167 pKa = 4.2DD1168 pKa = 3.38PDD1170 pKa = 5.4GADD1173 pKa = 3.81GSDD1176 pKa = 3.18GSGIAGFEE1184 pKa = 4.16CSLDD1188 pKa = 3.6GEE1190 pKa = 4.76PFSSCSSPKK1199 pKa = 9.76TYY1201 pKa = 10.92SSLGDD1206 pKa = 3.6GNHH1209 pKa = 5.92TFQVRR1214 pKa = 11.84SVDD1217 pKa = 3.45AAGNTDD1223 pKa = 4.84DD1224 pKa = 5.56SPEE1227 pKa = 4.23SYY1229 pKa = 8.6TWQIDD1234 pKa = 3.37TSLPEE1239 pKa = 4.05TTILSSPANLTTSTDD1254 pKa = 3.13AVFEE1258 pKa = 4.26FEE1260 pKa = 4.77GDD1262 pKa = 3.68DD1263 pKa = 3.68PGGSGIEE1270 pKa = 4.09NLEE1273 pKa = 4.11CMLDD1277 pKa = 3.26SSTFSVCTSPKK1288 pKa = 9.96EE1289 pKa = 4.09YY1290 pKa = 10.57NALPDD1295 pKa = 3.93GSHH1298 pKa = 6.17NFQVRR1303 pKa = 11.84AVDD1306 pKa = 3.32TAGNVDD1312 pKa = 4.29PSPAEE1317 pKa = 3.99INWTIDD1323 pKa = 3.03TTAPTVLISSTEE1335 pKa = 3.89SDD1337 pKa = 3.6PTNVNPIPITVQFSEE1352 pKa = 4.42PVTGFEE1358 pKa = 4.6EE1359 pKa = 4.48SDD1361 pKa = 2.95IMYY1364 pKa = 9.16HH1365 pKa = 5.71QRR1367 pKa = 11.84LPRR1370 pKa = 11.84SGQFHH1375 pKa = 6.71VFLQFDD1381 pKa = 3.88PEE1383 pKa = 4.06QRR1385 pKa = 11.84RR1386 pKa = 11.84DD1387 pKa = 3.17SYY1389 pKa = 11.32PRR1391 pKa = 11.84YY1392 pKa = 9.81SSSCRR1397 pKa = 11.84RR1398 pKa = 3.43

Molecular weight: 144.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K8PAL5|A0A0K8PAL5_9CHLR Ribokinase OS=Flexilinea flocculi OX=1678840 GN=rbsK PE=3 SV=1
MM1 pKa = 7.55NSNTNHH7 pKa = 6.16IQTCYY12 pKa = 10.27KK13 pKa = 9.91HH14 pKa = 6.66PNRR17 pKa = 11.84QTVLRR22 pKa = 11.84CNQCGRR28 pKa = 11.84PICLDD33 pKa = 3.66CAVSTPTGYY42 pKa = 10.06RR43 pKa = 11.84CKK45 pKa = 10.67EE46 pKa = 4.23CIKK49 pKa = 9.5EE50 pKa = 3.84QQRR53 pKa = 11.84RR54 pKa = 11.84FNNSIPRR61 pKa = 11.84DD62 pKa = 3.68YY63 pKa = 11.12IFAGLIAFILGLAGSYY79 pKa = 10.15FLIVLRR85 pKa = 11.84ILSGIFALLLGPSIGMLVVKK105 pKa = 9.44LVRR108 pKa = 11.84AVTQKK113 pKa = 10.36RR114 pKa = 11.84RR115 pKa = 11.84SSTLNHH121 pKa = 5.19VTVIAAGIGAGLPLLSNVFRR141 pKa = 11.84ILQSIVVGNFRR152 pKa = 11.84LLFSNTLEE160 pKa = 4.39IGWGVAFLVLLCSVILSNMKK180 pKa = 10.04GFSIRR185 pKa = 11.84RR186 pKa = 3.66

Molecular weight: 20.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2774

0

2774

989891

39

18250

356.8

39.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.227 ± 0.05

1.219 ± 0.023

5.351 ± 0.047

6.213 ± 0.06

4.96 ± 0.049

6.811 ± 0.086

1.823 ± 0.024

8.589 ± 0.048

5.537 ± 0.064

9.699 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.035

4.614 ± 0.051

4.179 ± 0.043

3.796 ± 0.034

4.404 ± 0.055

6.787 ± 0.068

5.336 ± 0.091

6.142 ± 0.033

1.269 ± 0.021

3.461 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski