Hubei tombus-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGT5|A0A1L3KGT5_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 15 OX=1923261 PE=4 SV=1
MM1 pKa = 7.27FLKK4 pKa = 10.37ADD6 pKa = 3.78KK7 pKa = 11.04AHH9 pKa = 6.35VFTDD13 pKa = 3.99GNPDD17 pKa = 3.73FGAPRR22 pKa = 11.84CIQYY26 pKa = 10.54RR27 pKa = 11.84NKK29 pKa = 10.43RR30 pKa = 11.84YY31 pKa = 9.56CLRR34 pKa = 11.84LATYY38 pKa = 7.19LHH40 pKa = 5.99PVEE43 pKa = 5.09RR44 pKa = 11.84EE45 pKa = 3.94LYY47 pKa = 9.84KK48 pKa = 11.0KK49 pKa = 10.6LDD51 pKa = 3.41ISGTPIFAKK60 pKa = 10.25GRR62 pKa = 11.84NLVQRR67 pKa = 11.84GQDD70 pKa = 2.87LWAKK74 pKa = 8.71FQHH77 pKa = 5.75FRR79 pKa = 11.84NPTIICMDD87 pKa = 3.57HH88 pKa = 6.72SKK90 pKa = 10.77FDD92 pKa = 3.24AHH94 pKa = 6.88FGVEE98 pKa = 4.12LLRR101 pKa = 11.84LEE103 pKa = 4.29HH104 pKa = 6.82KK105 pKa = 10.07FYY107 pKa = 11.4KK108 pKa = 10.83SMFPKK113 pKa = 10.17CDD115 pKa = 3.4RR116 pKa = 11.84EE117 pKa = 4.06EE118 pKa = 4.09LSRR121 pKa = 11.84LLEE124 pKa = 3.82MQINNKK130 pKa = 9.73GSTKK134 pKa = 10.4HH135 pKa = 4.03GTKK138 pKa = 10.12YY139 pKa = 8.77RR140 pKa = 11.84TRR142 pKa = 11.84GTRR145 pKa = 11.84CSGDD149 pKa = 3.23QNTGMGNSLGNYY161 pKa = 9.7CMLKK165 pKa = 10.67DD166 pKa = 4.03FADD169 pKa = 3.76HH170 pKa = 6.8NGWEE174 pKa = 3.95ACFYY178 pKa = 11.37VDD180 pKa = 5.04GDD182 pKa = 4.01DD183 pKa = 3.62SVMIVEE189 pKa = 5.0GDD191 pKa = 3.81VEE193 pKa = 4.21VDD195 pKa = 3.23PKK197 pKa = 10.89FFKK200 pKa = 10.77QFGMATKK207 pKa = 10.26VDD209 pKa = 3.9VVTKK213 pKa = 9.52EE214 pKa = 3.86FQDD217 pKa = 3.53MEE219 pKa = 4.55FCQTRR224 pKa = 11.84AVFDD228 pKa = 3.95GVSWRR233 pKa = 11.84MVRR236 pKa = 11.84NPARR240 pKa = 11.84LLARR244 pKa = 11.84LPWAVQQVTPQCRR257 pKa = 11.84GKK259 pKa = 9.74YY260 pKa = 9.03LRR262 pKa = 11.84SVGLCEE268 pKa = 3.61IALGVGLPIAQHH280 pKa = 6.18IGEE283 pKa = 4.76KK284 pKa = 10.26LSKK287 pKa = 10.25LGKK290 pKa = 10.28GYY292 pKa = 9.32MVTGNHH298 pKa = 5.06YY299 pKa = 8.12MASKK303 pKa = 10.45EE304 pKa = 4.12YY305 pKa = 10.22IRR307 pKa = 11.84PTRR310 pKa = 11.84ARR312 pKa = 11.84LIEE315 pKa = 4.05PTMEE319 pKa = 3.55ARR321 pKa = 11.84MSYY324 pKa = 9.94ARR326 pKa = 11.84AWGISVEE333 pKa = 4.08EE334 pKa = 4.03QLRR337 pKa = 11.84IEE339 pKa = 4.25TVEE342 pKa = 3.84IEE344 pKa = 4.17LPEE347 pKa = 5.66LGDD350 pKa = 3.76LRR352 pKa = 11.84TFDD355 pKa = 3.8EE356 pKa = 4.59VPYY359 pKa = 9.56RR360 pKa = 11.84TLSS363 pKa = 3.22

Molecular weight: 41.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGX0|A0A1L3KGX0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 15 OX=1923261 PE=4 SV=1
MM1 pKa = 7.35NKK3 pKa = 9.98SSTGVSTSSLGHH15 pKa = 6.0GKK17 pKa = 10.7GPGQGSHH24 pKa = 5.84RR25 pKa = 11.84VRR27 pKa = 11.84EE28 pKa = 4.15AGANRR33 pKa = 11.84NKK35 pKa = 10.31RR36 pKa = 11.84CSLGVKK42 pKa = 10.0SGGKK46 pKa = 4.82TTNTTGHH53 pKa = 7.08PPAAGWIHH61 pKa = 6.27PQPAANAAAKK71 pKa = 10.01AAATDD76 pKa = 3.97PNKK79 pKa = 10.15IRR81 pKa = 11.84VKK83 pKa = 10.65EE84 pKa = 3.93RR85 pKa = 11.84TTGNGAKK92 pKa = 9.78QGSQQSLLSKK102 pKa = 10.44HH103 pKa = 5.67RR104 pKa = 11.84WSKK107 pKa = 10.48EE108 pKa = 3.63DD109 pKa = 3.38LRR111 pKa = 11.84AAAARR116 pKa = 11.84TLRR119 pKa = 11.84ARR121 pKa = 11.84VKK123 pKa = 9.99QLEE126 pKa = 4.33GEE128 pKa = 4.23LEE130 pKa = 3.95ALRR133 pKa = 11.84KK134 pKa = 9.23QFKK137 pKa = 10.13QSSTGKK143 pKa = 10.31SGGSTPKK150 pKa = 10.57RR151 pKa = 11.84NVDD154 pKa = 3.31KK155 pKa = 10.8PGSKK159 pKa = 10.35VGGKK163 pKa = 8.98QSNQVPKK170 pKa = 10.67SSPPNKK176 pKa = 9.81KK177 pKa = 10.07KK178 pKa = 10.59GDD180 pKa = 3.45RR181 pKa = 11.84RR182 pKa = 11.84TSGRR186 pKa = 11.84PEE188 pKa = 4.15DD189 pKa = 4.07RR190 pKa = 11.84APPAPQEE197 pKa = 3.91QSRR200 pKa = 11.84GDD202 pKa = 3.56RR203 pKa = 11.84TRR205 pKa = 11.84KK206 pKa = 9.39RR207 pKa = 11.84HH208 pKa = 5.11TAPAPPVPPGPSSAMPAKK226 pKa = 10.15VSRR229 pKa = 11.84GTGTAPPAAEE239 pKa = 4.21VTPDD243 pKa = 3.48PAKK246 pKa = 10.58DD247 pKa = 3.43KK248 pKa = 11.26KK249 pKa = 10.54RR250 pKa = 11.84DD251 pKa = 3.34LPQYY255 pKa = 10.35SGPSYY260 pKa = 10.88AATVKK265 pKa = 10.63SGLCTEE271 pKa = 4.28SNKK274 pKa = 10.53PIFSKK279 pKa = 10.67EE280 pKa = 3.57CAIIDD285 pKa = 3.53NSIIARR291 pKa = 11.84SAKK294 pKa = 9.89RR295 pKa = 11.84SMRR298 pKa = 11.84KK299 pKa = 9.05LDD301 pKa = 3.72HH302 pKa = 5.84QWADD306 pKa = 3.0KK307 pKa = 10.81EE308 pKa = 4.36LTHH311 pKa = 6.6FLLMEE316 pKa = 4.38FAFTPRR322 pKa = 11.84TTEE325 pKa = 3.7VLRR328 pKa = 11.84LMHH331 pKa = 6.93GRR333 pKa = 11.84LQKK336 pKa = 10.42HH337 pKa = 5.96LRR339 pKa = 11.84TFDD342 pKa = 3.09TSAYY346 pKa = 7.26TQEE349 pKa = 4.0EE350 pKa = 5.2MYY352 pKa = 10.07KK353 pKa = 10.43LSVRR357 pKa = 11.84TVEE360 pKa = 3.78AAIRR364 pKa = 11.84VPQAEE369 pKa = 4.09QAVRR373 pKa = 11.84AGLKK377 pKa = 9.91NDD379 pKa = 3.43SALEE383 pKa = 3.9EE384 pKa = 3.95VRR386 pKa = 11.84KK387 pKa = 10.21NNKK390 pKa = 9.12FLEE393 pKa = 4.66SGLVGNVRR401 pKa = 11.84GLLGNKK407 pKa = 8.79KK408 pKa = 9.84LKK410 pKa = 10.28EE411 pKa = 4.1MPGKK415 pKa = 10.51SRR417 pKa = 3.7

Molecular weight: 45.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1129

349

417

376.3

41.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.149 ± 1.039

1.594 ± 0.45

3.809 ± 0.7

5.049 ± 0.725

2.923 ± 0.797

8.415 ± 0.255

1.86 ± 0.597

3.012 ± 0.518

6.289 ± 2.352

7.44 ± 0.469

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.482

4.34 ± 0.525

6.377 ± 0.785

4.074 ± 0.113

7.972 ± 0.378

7.352 ± 1.113

8.06 ± 1.716

6.997 ± 1.417

1.417 ± 0.404

2.391 ± 0.679
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski