Rhodosalinus sp. E84

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodosalinus; unclassified Rhodosalinus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A365U609|A0A365U609_9RHOB Acyl-coenzyme A dehydrogenase OS=Rhodosalinus sp. E84 OX=2259333 GN=fadE PE=3 SV=1
DDD2 pKa = 3.79YY3 pKa = 11.44EEE5 pKa = 5.39GAGSDDD11 pKa = 4.01EE12 pKa = 4.41YYY14 pKa = 11.17MHHH17 pKa = 7.21DDD19 pKa = 4.24DD20 pKa = 3.83LLGEEE25 pKa = 4.91GRR27 pKa = 11.84DDD29 pKa = 3.4LYYY32 pKa = 10.68GYYY35 pKa = 10.82NDDD38 pKa = 3.14LLGGKKK44 pKa = 10.01DDD46 pKa = 4.51DD47 pKa = 3.92YY48 pKa = 11.94HHH50 pKa = 6.84GKKK53 pKa = 9.99DD54 pKa = 4.08DD55 pKa = 4.4DD56 pKa = 5.48VIADDD61 pKa = 4.11EE62 pKa = 5.13ADDD65 pKa = 3.86MWGGLDDD72 pKa = 3.19DDD74 pKa = 4.81DD75 pKa = 4.65VFGHHH80 pKa = 6.12VIDDD84 pKa = 4.03EEE86 pKa = 4.52HHH88 pKa = 5.2KK89 pKa = 9.68DDD91 pKa = 4.97EE92 pKa = 4.16GFDDD96 pKa = 3.2KK97 pKa = 11.46NVVKKK102 pKa = 10.8DD103 pKa = 4.34SVVGNDDD110 pKa = 2.81LVFHHH115 pKa = 6.7AYYY118 pKa = 10.03EE119 pKa = 4.36DD120 pKa = 3.71GEEE123 pKa = 4.27KKK125 pKa = 10.97EEE127 pKa = 4.09VDDD130 pKa = 3.81DDD132 pKa = 4.4DDD134 pKa = 3.95TANDDD139 pKa = 3.72KKK141 pKa = 10.36TATDDD146 pKa = 4.0QTHHH150 pKa = 6.27EE151 pKa = 4.5GSVIVEEE158 pKa = 4.5DD159 pKa = 3.84WSTLGLDDD167 pKa = 3.41VGYYY171 pKa = 9.25LRR173 pKa = 11.84PGDDD177 pKa = 5.07GFEEE181 pKa = 4.55DD182 pKa = 4.49LMEEE186 pKa = 4.85YY187 pKa = 10.47LNYYY191 pKa = 10.04DDD193 pKa = 3.87EE194 pKa = 4.37EEE196 pKa = 4.23GKKK199 pKa = 10.02VIRR202 pKa = 11.84FEEE205 pKa = 4.55DD206 pKa = 3.29KK207 pKa = 10.84VEEE210 pKa = 4.1KKK212 pKa = 10.74EE213 pKa = 4.26DDD215 pKa = 4.11PPVDDD220 pKa = 4.28DDD222 pKa = 4.22EEE224 pKa = 4.09

Molecular weight: 24.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A365U791|A0A365U791_9RHOB Purine nucleoside phosphorylase DeoD-type OS=Rhodosalinus sp. E84 OX=2259333 GN=deoD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3680

0

3680

1155616

28

2157

314.0

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.21 ± 0.08

0.808 ± 0.011

5.711 ± 0.036

6.791 ± 0.043

3.458 ± 0.024

9.079 ± 0.04

2.022 ± 0.022

4.347 ± 0.032

2.167 ± 0.035

10.314 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.022

1.921 ± 0.019

5.595 ± 0.034

2.741 ± 0.022

8.126 ± 0.045

4.299 ± 0.028

5.001 ± 0.025

7.314 ± 0.033

1.475 ± 0.018

2.051 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski