Bacillus phage vB_BcM_Sam112

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F749|A0A5Q2F749_9CAUD Portal protein OS=Bacillus phage vB_BcM_Sam112 OX=2663324 GN=Sam112_gp3 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 9.08LTPEE6 pKa = 3.98QIKK9 pKa = 10.5AIQEE13 pKa = 3.86LCEE16 pKa = 4.1RR17 pKa = 11.84CDD19 pKa = 3.28WSQEE23 pKa = 3.91KK24 pKa = 10.56AVDD27 pKa = 3.63DD28 pKa = 3.82TLEE31 pKa = 3.86ILGVYY36 pKa = 9.46NQVFGEE42 pKa = 4.32AEE44 pKa = 3.75

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2FA70|A0A5Q2FA70_9CAUD Uncharacterized protein OS=Bacillus phage vB_BcM_Sam112 OX=2663324 GN=Sam112_gp53 PE=4 SV=1
MM1 pKa = 7.09SKK3 pKa = 10.13KK4 pKa = 9.85RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.87DD9 pKa = 2.91GFIPVVRR16 pKa = 11.84MFLSIVRR23 pKa = 11.84IIKK26 pKa = 6.47TTQKK30 pKa = 10.19RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84NDD35 pKa = 2.9RR36 pKa = 11.84KK37 pKa = 9.41ATEE40 pKa = 3.72KK41 pKa = 9.87HH42 pKa = 6.03TINNN46 pKa = 3.63

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14018

35

1132

186.9

20.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.732 ± 0.576

0.592 ± 0.096

5.664 ± 0.238

7.555 ± 0.575

3.852 ± 0.164

6.891 ± 0.32

1.726 ± 0.163

6.834 ± 0.254

8.503 ± 0.583

7.212 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.025 ± 0.25

5.172 ± 0.344

3.174 ± 0.189

3.874 ± 0.161

4.166 ± 0.339

5.072 ± 0.3

6.335 ± 0.425

6.592 ± 0.253

1.434 ± 0.191

3.595 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski